WASF2 Antibody - #DF4618

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Product: WASF2 Antibody
Catalog: DF4618
Description: Rabbit polyclonal antibody to WASF2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 54 KD; 54kD(Calculated).
Uniprot: Q9Y6W5
RRID: AB_2836982

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
WASF2 Antibody detects endogenous levels of total WASF2.
RRID:
AB_2836982
Cite Format: Affinity Biosciences Cat# DF4618, RRID:AB_2836982.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DICTYOSTELIUM; dJ393P12.2; IMD2; Protein WAVE-2; Putative Wiskott Aldrich syndrome protein family member 4; SCAR 2; SCAR; SCAR2; Suppressor of cyclic-AMP receptor (WASP family); Verprolin homology domain-containing protein 2; WASF2; WASF2_HUMAN; WASF4; WASP family protein member 2; WASP family protein member 4; WASP family Verprolin homologous protein 2; WASP-family protein member 2; WAVE 2; WAVE2; Wiskott-Aldrich syndrome protein family member 2; Wiskott-Aldrich syndrome protein family verprolin-homologous protein;

Immunogens

Immunogen:
Uniprot:

Q9Y6W5(WASF2_HUMAN) >>Visit HPA database.

Gene(ID):
WASF2(10163)
Expression:
Q9Y6W5 WASF2_HUMAN:

Expressed in all tissues with strongest expression in placenta, lung, and peripheral blood leukocytes, but not in skeletal muscle.

Sequence:
MPLVTRNIEPRHLCRQTLPSVRSELECVTNITLANVIRQLGSLSKYAEDIFGELFTQANTFASRVSSLAERVDRLQVKVTQLDPKEEEVSLQGINTRKAFRSSTIQDQKLFDRNSLPVPVLETYNTCDTPPPLNNLTPYRDDGKEALKFYTDPSYFFDLWKEKMLQDTKDIMKEKRKHRKEKKDNPNRGNVNPRKIKTRKEEWEKMKMGQEFVESKEKLGTSGYPPTLVYQNGSIGCVENVDASSYPPPPQSDSASSPSPSFSEDNLPPPPAEFSYPVDNQRGSGLAGPKRSSVVSPSHPPPAPPLGSPPGPKPGFAPPPAPPPPPPPMIGIPPPPPPVGFGSPGTPPPPSPPSFPPHPDFAAPPPPPPPPAADYPTLPPPPLSQPTGGAPPPPPPPPPPGPPPPPFTGADGQPAIPPPLSDTTKPKSSLPAVSDARSDLLSAIRQGFQLRRVEEQREQEKRDVVGNDVATILSRRIAVEYSDSEDDSSEFDEDDWSD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
91
Zebrafish
91
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6W5 As Substrate

Site PTM Type Enzyme
T17 Phosphorylation
T80 Phosphorylation
K85 Ubiquitination
S90 Phosphorylation
S103 Phosphorylation
K109 Ubiquitination
Y124 Phosphorylation
T129 Phosphorylation
T137 Phosphorylation
Y139 Phosphorylation
Y150 Phosphorylation A0A173G4P4 (Abl fusion) , P00519 (ABL1)
Y155 Phosphorylation
K161 Ubiquitination
T168 Phosphorylation
K169 Ubiquitination
S215 Phosphorylation
K216 Ubiquitination
Y230 Phosphorylation
S257 Phosphorylation
S259 Phosphorylation
S284 Phosphorylation
S292 Phosphorylation
S293 Phosphorylation
S296 Phosphorylation P28482 (MAPK1)
S298 Phosphorylation
S308 Phosphorylation P28482 (MAPK1)
S343 Phosphorylation P27361 (MAPK3)
T346 Phosphorylation P27361 (MAPK3)
S351 Phosphorylation P27361 (MAPK3)
S429 Phosphorylation
S442 Phosphorylation
T471 Phosphorylation
S474 Phosphorylation
S482 Phosphorylation P68400 (CSNK2A1)
S484 Phosphorylation P68400 (CSNK2A1)
S488 Phosphorylation P68400 (CSNK2A1)
S489 Phosphorylation P68400 (CSNK2A1)
S497 Phosphorylation P68400 (CSNK2A1)

Research Backgrounds

Function:

Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell projection>Lamellipodium.
Note: At the interface between the lamellipodial actin meshwork and the membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues with strongest expression in placenta, lung, and peripheral blood leukocytes, but not in skeletal muscle.

Subunit Structure:

Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 (NCKAP1l/HEM1 in hematopoietic cells) and WASF2/WAVE2. Directly interacts with BRK1. Interacts with FNBP1L (via the SH3 domain).

(Microbial infection) Interacts with human cytomegalovirus protein UL135.

Family&Domains:

Binds and activates the Arp2/3 complex via the C-terminal domain. Interacts with actin via the WH2 domain.

Belongs to the SCAR/WAVE family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

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