WEE2 Antibody - #DF4617
| Product: | WEE2 Antibody |
| Catalog: | DF4617 |
| Description: | Rabbit polyclonal antibody to WEE2 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Horse, Rabbit, Dog |
| Mol.Wt.: | 60 KD; 63kD(Calculated). |
| Uniprot: | P0C1S8 |
| RRID: | AB_2836981 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4617, RRID:AB_2836981.
Fold/Unfold
hCG_39453; WEE1 homolog 2 (S. pombe); Wee1-like protein kinase 1B; Wee1-like protein kinase 2; WEE1B; Wee1B kinase; Wee2; WEE2_HUMAN;
Immunogens
Expressed in oocytes (at protein level) (PubMed:29606300). May also be expressed in testis (PubMed:11029659).
- P0C1S8 WEE2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDDKDIDKELRQKLNFSYCEETEIEGQKKVEESREASSQTPEKGEVQDSEAKGTPPWTPLSNVHELDTSSEKDKESPDQILRTPVSHPLKCPETPAQPDSRSKLLPSDSPSTPKTMLSRLVISPTGKLPSRGPKHLKLTPAPLKDEMTSLALVNINPFTPESYKKLFLQSGGKRKIRGDLEEAGPEEGKGGLPAKRCVLRETNMASRYEKEFLEVEKIGVGEFGTVYKCIKRLDGCVYAIKRSMKTFTELSNENSALHEVYAHAVLGHHPHVVRYYSSWAEDDHMIIQNEYCNGGSLQAAISENTKSGNHFEEPKLKDILLQISLGLNYIHNSSMVHLDIKPSNIFICHKMQSESSGVIEEVENEADWFLSANVMYKIGDLGHATSINKPKVEEGDSRFLANEILQEDYRHLPKADIFALGLTIAVAAGAESLPTNGAAWHHIRKGNFPDVPQELSESFSSLLKNMIQPDAEQRPSAAALARNTVLRPSLGKTEELQQQLNLEKFKTATLERELREAQQAQSPQGYTHHGDTGVSGTHTGSRSTKRLVGGKSARSSSFTSGEREPLH
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P0C1S8 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T83 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| T225 | Phosphorylation | Uniprot | |
| K389 | Acetylation | Uniprot | |
| T509 | Phosphorylation | Uniprot |
Research Backgrounds
Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1/CDC2 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation (By similarity).
Phosphorylated on serine residues. Phosphorylation leads to increase its activity (By similarity).
Nucleus.
Expressed in oocytes (at protein level). May also be expressed in testis.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
Research Fields
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.