NSF Antibody - #DF4611
| Product: | NSF Antibody |
| Catalog: | DF4611 |
| Description: | Rabbit polyclonal antibody to NSF |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 75 KD; 83kD(Calculated). |
| Uniprot: | P46459 |
| RRID: | AB_2836975 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4611, RRID:AB_2836975.
Fold/Unfold
N ethylmaleimide sensitive factor; N ethylmaleimide sensitive factor like protein; N ethylmaleimide sensitive fusion protein; N-ethylmaleimide-sensitive fusion protein; NEM sensitive fusion protein; NEM-sensitive fusion protein; NSF; NSF_HUMAN; SKD 2; SKD2; Vesicle fusing ATPase; Vesicle-fusing ATPase; Vesicular fusion protein NSF; Vesicular-fusion protein NSF;
Immunogens
- P46459 NSF_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGRSMQAARCPTDELSLTNCAVVNEKDFQSGQHVIVRTSPNHRYTFTLKTHPSVVPGSIAFSLPQRKWAGLSIGQEIEVSLYTFDKAKQCIGTMTIEIDFLQKKSIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNEKLFGLLVKDIEAMDPSILKGEPATGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFSTTIHVPNIATGEQLLEALELLGNFKDKERTTIAQQVKGKKVWIGIKKLLMLIEMSLQMDPEYRVRKFLALLREEGASPLDFD
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P46459 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| C21 | S-Nitrosylation | Uniprot | |
| Y45 | Phosphorylation | Uniprot | |
| Y83 | Phosphorylation | P16591 (FER) , P07332 (FES) | Uniprot |
| C91 | S-Nitrosylation | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| Y112 | Phosphorylation | Uniprot | |
| T114 | Phosphorylation | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| T166 | Phosphorylation | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| C250 | S-Nitrosylation | Uniprot | |
| K254 | Ubiquitination | Uniprot | |
| C264 | S-Nitrosylation | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| R337 | Methylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| T349 | Phosphorylation | Uniprot | |
| S356 | Phosphorylation | Uniprot | |
| K401 | Ubiquitination | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Q02156 (PRKCE) | Uniprot |
| T461 | Phosphorylation | Q02156 (PRKCE) | Uniprot |
| K462 | Ubiquitination | Uniprot | |
| K469 | Ubiquitination | Uniprot | |
| S472 | Phosphorylation | Uniprot | |
| K529 | Ubiquitination | Uniprot | |
| T534 | Phosphorylation | Uniprot | |
| S547 | Phosphorylation | Uniprot | |
| K555 | Ubiquitination | Uniprot | |
| K566 | Ubiquitination | Uniprot | |
| S569 | Phosphorylation | Uniprot | |
| K572 | Ubiquitination | Uniprot | |
| K581 | Ubiquitination | Uniprot | |
| K587 | Ubiquitination | Uniprot | |
| Y611 | Phosphorylation | Uniprot | |
| T645 | Phosphorylation | Q5S007 (LRRK2) | Uniprot |
| T646 | Phosphorylation | Uniprot | |
| S647 | Phosphorylation | Uniprot | |
| T693 | Phosphorylation | Uniprot | |
| K699 | Ubiquitination | Uniprot | |
| S717 | Phosphorylation | Uniprot | |
| Y724 | Phosphorylation | Uniprot | |
| S739 | Phosphorylation | Uniprot |
Research Backgrounds
Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).
Phosphorylation at Ser-569 interferes with homohexamerization.
Cytoplasm.
Homohexamer. Interacts with GABARAP and GABARAPL2. Interacts with GRIA2. Interacts with PLK2, leading to disrupt the interaction with GRIA2. Interacts with MUSK; may regulate MUSK endocytosis and activity (By similarity). Interacts with CDK16 (By similarity).
Belongs to the AAA ATPase family.
Research Fields
· Organismal Systems > Nervous system > Synaptic vesicle cycle.
· Organismal Systems > Nervous system > GABAergic synapse.
· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.
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