VTI1B Antibody - #DF4610

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Product: VTI1B Antibody
Catalog: DF4610
Description: Rabbit polyclonal antibody to VTI1B
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 27 KD; 27kD(Calculated).
Uniprot: Q9UEU0
RRID: AB_2836974

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(91%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
VTI1B Antibody detects endogenous levels of total VTI1B.
RRID:
AB_2836974
Cite Format: Affinity Biosciences Cat# DF4610, RRID:AB_2836974.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HGNC:17793; V SNARE; v-SNARE; Vesicle associated soluble NSF attachment protein receptor; Vesicle transport through interaction with t SNAREs 1B; Vesicle transport through interaction with t SNAREs homolog 1B (yeast); Vesicle transport through interaction with t-SNAREs homolog 1B; Vesicle transport v-SNARE protein Vti1-like 1; VTI1; VTI1 like; Vti1 rp1; VTI1-LIKE; Vti1-rp1; VTI1B; VTI1B_HUMAN; VTI1L; VTI1L1; VTI2;

Immunogens

Immunogen:
Uniprot:

Q9UEU0(VTI1B_HUMAN) >>Visit HPA database.

Gene(ID):
VTI1B(10490)
Expression:
Q9UEU0 VTI1B_HUMAN:

Expressed in all tissues examined.

Sequence:
MASSAASSEHFEKLHEIFRGLHEDLQGVPERLLGTAGTEEKKKLIRDFDEKQQEANETLAEMEEELRYAPLSFRNPMMSKLRNYRKDLAKLHREVRSTPLTATPGGRGDMKYGIYAVENEHMNRLQSQRAMLLQGTESLNRATQSIERSHRIATETDQIGSEIIEELGEQRDQLERTKSRLVNTSENLSKSRKILRSMSRKVTTNKLLLSIIILLELAILGGLVYYKFFRSH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
91
Xenopus
82
Zebrafish
73
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UEU0 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K13 Ubiquitination
K51 Ubiquitination
S72 Phosphorylation
K90 Ubiquitination
T103 Phosphorylation
R107 Methylation
Y112 Phosphorylation
Y115 Phosphorylation
S127 Phosphorylation
S138 Phosphorylation
T154 Phosphorylation
T156 Phosphorylation
S161 Phosphorylation
S179 Phosphorylation
S185 Phosphorylation
S189 Phosphorylation
K190 Ubiquitination
T203 Phosphorylation
S210 Phosphorylation
Y225 Phosphorylation

Research Backgrounds

Function:

V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence.

Subcellular Location:

Early endosome membrane>Single-pass type IV membrane protein. Late endosome membrane>Single-pass type IV membrane protein. Lysosome membrane. Cytoplasmic granule. Recycling endosome membrane>Single-pass type IV membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues examined.

Subunit Structure:

Forms a SNARE complex with STX7, STX8 and VAMP8 which functions in the homotypic fusion of late endosomes. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VIT1B that is required for heterotypic fusion of late endosomes with lysosomes (By similarity). May interact with STX17. Interacts with CLINT1.

Family&Domains:

Belongs to the VTI1 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.

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