UBE2T Antibody | Affinity Biosciences

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Western blot analysis of extracts from A2780 cells, using UBE2T Antibody.

Product:	UBE2T Antibody
Catalog:	DF4601
Description:	Rabbit polyclonal antibody to UBE2T
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	22 KD; 23kD(Calculated).
Uniprot:	Q9NPD8
RRID:	AB_2836965

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(90%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(90%)

Clonality:

Polyclonal

Specificity:

UBE2T Antibody detects endogenous levels of total UBE2T.

RRID:

AB_2836965
Cite Format: Affinity Biosciences Cat# DF4601, RRID:AB_2836965.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Cell proliferation inducing gene 50 protein; Cell proliferation-inducing gene 50 protein; HSPC150; HSPC150 protein similar to ubiquitin conjugating enzyme; PIG50; Ube2t; UBE2T_HUMAN; Ubiquitin carrier protein T; Ubiquitin conjugating enzyme; ubiquitin conjugating enzyme E2 T; Ubiquitin conjugating enzyme E2T; Ubiquitin protein ligase T; Ubiquitin-conjugating enzyme E2 T; Ubiquitin-protein ligase T;

Immunogens

Immunogen:

Uniprot:

Q9NPD8(UBE2T_HUMAN) >>Visit HPA database.

Gene(ID):

UBE2T(29089)

Sequence:

Q9NPD8 UBE2T_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MQRASRLKRELHMLATEPPPGITCWQDKDQMDDLRAQILGGANTPYEKGVFKLEVIIPERYPFEPPQIRFLTPIYHPNIDSAGRICLDVLKLPPKGAWRPSLNIATVLTSIQLLMSEPNPDDPLMADISSEFKYNKPAFLKNARQWTEKHARQKQKADEEEMLDNLPEAGDSRVHNSTQKRKASQLVGIEKKFHPDV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Dog

100

Zebrafish

100

Chicken

100

Rabbit

100

Sheep

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NPD8 As Substrate

Q9NPD8

Site	PTM Type	Source
K28	Ubiquitination	Uniprot
K48	Ubiquitination	Uniprot
T72	Phosphorylation	Uniprot
K91	Ubiquitination	Uniprot
K136	Ubiquitination	Uniprot
K141	Ubiquitination	Uniprot
K156	Ubiquitination	Uniprot
K182	Ubiquitination	Uniprot
S184	Phosphorylation	Uniprot
K191	Acetylation	Uniprot
K191	Ubiquitination	Uniprot
K192	Ubiquitination	Uniprot

Research Backgrounds

Q9NPD8_UBE2T_HUMAN

Function:

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination.

PTMs:

Auto-ubiquitinated. Effects of auto-monoubiquitination at Lys-91 and Lys-182 are unclear: according to a report, monoubiquitination inactivates E2 enzyme activity. In contrast, according to another report, autoubiquitination does not affect E2 enzyme activity.

Subcellular Location:

Nucleus.
Note: Accumulates to chromatin.

Subunit Structure:

Directly interacts with FANCL. Interacts with BRCA1.

Family&Domains:

Belongs to the ubiquitin-conjugating enzyme family.

Research Fields

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

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UBE2T Antibody - #DF4601