UBAP2L Antibody - #DF4599

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Product: UBAP2L Antibody
Catalog: DF4599
Description: Rabbit polyclonal antibody to UBAP2L
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 115 KD; 115kD(Calculated).
Uniprot: Q14157
RRID: AB_2836963

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
UBAP2L Antibody detects endogenous levels of total UBAP2L.
RRID:
AB_2836963
Cite Format: Affinity Biosciences Cat# DF4599, RRID:AB_2836963.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ42300; KIAA0144; NICE 4; NICE4; Protein NICE-4; Protein NICE4; Ubap2l; Ubiquitin associated protein 2 like; Ubiquitin-associated protein 2-like; UBP2L_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q14157(UBP2L_HUMAN) >>Visit HPA database.

Gene(ID):
UBAP2L(9898)
Expression:
Q14157 UBP2L_HUMAN:

Ubiquitous.

Sequence:
MMTSVGTNRARGNWEQPQNQNQTQHKQRPQATAEQIRLAQMISDHNDADFEEKVKQLIDITGKNQDECVIALHDCNGDVNRAINVLLEGNPDTHSWEMVGKKKGVSGQKDGGQTESNEEGKENRDRDRDYSRRRGGPPRRGRGASRGREFRGQENGLDGTKSGGPSGRGTERGRRGRGRGRGGSGRRGGRFSAQGMGTFNPADYAEPANTDDNYGNSSGNTWNNTGHFEPDDGTSAWRTATEEWGTEDWNEDLSETKIFTASNVSSVPLPAENVTITAGQRIDLAVLLGKTPSTMENDSSNLDPSQAPSLAQPLVFSNSKQTAISQPASGNTFSHHSMVSMLGKGFGDVGEAKGGSTTGSQFLEQFKTAQALAQLAAQHSQSGSTTTSSWDMGSTTQSPSLVQYDLKNPSDSAVHSPFTKRQAFTPSSTMMEVFLQEKSPAVATSTAAPPPPSSPLPSKSTSAPQMSPGSSDNQSSSPQPAQQKLKQQKKKASLTSKIPALAVEMPGSADISGLNLQFGALQFGSEPVLSDYESTPTTSASSSQAPSSLYTSTASESSSTISSNQSQESGYQSGPIQSTTYTSQNNAQGPLYEQRSTQTRRYPSSISSSPQKDLTQAKNGFSSVQATQLQTTQSVEGATGSAVKSDSPSTSSIPPLNETVSAASLLTTTNQHSSSLGGLSHSEEIPNTTTTQHSSTLSTQQNTLSSSTSSGRTSTSTLLHTSVESEANLHSSSSTFSTTSSTVSAPPPVVSVSSSLNSGSSLGLSLGSNSTVTASTRSSVATTSGKAPPNLPPGVPPLLPNPYIMAPGLLHAYPPQVYGYDDLQMLQTRFPLDYYSIPFPTPTTPLTGRDGSLASNPYSGDLTKFGRGDASSPAPATTLAQPQQNQTQTHHTTQQTFLNPALPPGYSYTSLPYYTGVPGLPSTFQYGPAVFPVAPTSSKQHGVNVSVNASATPFQQPSGYGSHGYNTGVSVTSSNTGVPDISGSVYSKTQQSFEKQGFHSGTPAASFNLPSALGSGGPINPATAAAYPPAPFMHILTPHQQPHSQILHHHLQQDGQTGSGQRSQTSSIPQKPQTNKSAYNSYSWGAN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
73
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14157 As Substrate

Site PTM Type Enzyme
M1 Acetylation
T7 Phosphorylation
R11 Methylation
K26 Ubiquitination
S43 Phosphorylation
S95 Phosphorylation
K101 Ubiquitination
K109 Ubiquitination
S116 Phosphorylation
K121 Ubiquitination
Y130 Phosphorylation
S131 Phosphorylation
R151 Methylation
R168 Methylation
R172 Methylation
R174 Methylation
S184 Phosphorylation
R187 Methylation
R190 Methylation
Y204 Phosphorylation
Y214 Phosphorylation
T225 Phosphorylation
T239 Phosphorylation
T241 Phosphorylation
T246 Phosphorylation
S254 Phosphorylation
S262 Phosphorylation
S266 Phosphorylation
K320 Ubiquitination
S334 Phosphorylation
K344 Ubiquitination
K353 Ubiquitination
S356 Phosphorylation
S360 Phosphorylation
K367 Ubiquitination
S380 Phosphorylation
S382 Phosphorylation
S389 Phosphorylation
S398 Phosphorylation
S400 Phosphorylation
Y404 Phosphorylation
S410 Phosphorylation
S412 Phosphorylation
S416 Phosphorylation
T419 Phosphorylation
K420 Methylation
K420 Ubiquitination
T425 Phosphorylation
S427 Phosphorylation
S428 Phosphorylation
T429 Phosphorylation
S439 Phosphorylation
S445 O-Glycosylation
T446 Phosphorylation
S453 Phosphorylation
S454 Phosphorylation P06493 (CDK1)
S458 Phosphorylation
S460 Phosphorylation
T461 Phosphorylation
S462 Phosphorylation
S467 Phosphorylation
S470 Phosphorylation
S471 Phosphorylation
S475 Phosphorylation
S476 Phosphorylation
S477 Phosphorylation
S493 Phosphorylation
T495 Phosphorylation
S496 Phosphorylation
Y571 Phosphorylation
S573 Phosphorylation
Y581 Phosphorylation
Y592 Phosphorylation
Y602 Phosphorylation
S604 Phosphorylation
S605 Phosphorylation
S607 Phosphorylation
S608 Phosphorylation
S609 Phosphorylation
K612 Acetylation
K612 Ubiquitination
T615 Phosphorylation
S634 Phosphorylation
T713 Phosphorylation
S714 Phosphorylation
S732 Phosphorylation
S740 Phosphorylation
T782 O-Glycosylation
T783 O-Glycosylation
Y803 Phosphorylation
Y813 Phosphorylation
Y820 Phosphorylation
R829 Methylation
Y834 Phosphorylation
Y835 Phosphorylation
S836 Phosphorylation
T841 Phosphorylation
T843 Phosphorylation
T844 Phosphorylation
T847 Phosphorylation
S852 Phosphorylation
S855 Phosphorylation
Y858 Phosphorylation
S859 Phosphorylation
T863 Phosphorylation
K864 Ubiquitination
S992 Phosphorylation
K1071 Ubiquitination
S1077 Phosphorylation
Y1079 Phosphorylation
S1081 Phosphorylation
Y1082 Phosphorylation

Research Backgrounds

Function:

Plays an important role in the activity of long-term repopulating hematopoietic stem cells (LT-HSCs).

PTMs:

Acetylated.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with BMI1. Part of a complex consisting of UBAP2L, BMI1 and RNF2.

References

1). Ubiquitin-associated protein 2 like (UBAP2L) enhances growth and metastasis of gastric cancer cells. Bioengineered, 2021 (PubMed: 34823423) [IF=4.2]

Application: WB    Species: Human    Sample: gastric cancer cells

Figure 3. Silencing of UBAP2L repressed proliferation and promoted apoptosis of gastric cancer cells a and b. The mRNA and protein levels of UBAP2L in normal human gastric mucosa cells and gastric cancer cells were determined by real-time PCR and western blot, respectively. c and d. The mRNA and protein levels of UBAP2L in AGS and NCI-N87 cells were detected after transfection of siRNA targeting UBAP2L. e and f. CCK-8 assay was performed for measurement of cell viability in UBAP2L-silenced AGS and NCI-N87 cells. g-i. The cell cycle distribution of AGS and NCI-N87 cells was examined by flow cytometry. j. The proliferation index (PI) of AGS and NCI-N87 cells were calculated based on the cell numbers in each phase of the cell cycle (PI = (cell numbers in G2/M and S phases)/cell numbers in G1/G0 phase). k and l. The apoptosis of AGS and NCI-N87 cells were detected by flow cytometry. (*p < 0.05, **p < 0.01, ***p < 0.001, compared with siRNA NC)

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