UBAC1 Antibody - #DF4598

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Product: UBAC1 Antibody
Catalog: DF4598
Description: Rabbit polyclonal antibody to UBAC1
Application: WB IF/ICC
Reactivity: Human
Prediction: Zebrafish, Horse, Dog, Chicken, Xenopus
Mol.Wt.: 45 KD; 45kD(Calculated).
Uniprot: Q9BSL1
RRID: AB_2836962

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Zebrafish(80%), Horse(80%), Dog(80%), Chicken(80%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
UBAC1 Antibody detects endogenous levels of total UBAC1.
RRID:
AB_2836962
Cite Format: Affinity Biosciences Cat# DF4598, RRID:AB_2836962.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1110033G07Rik; AA407978; E3 ubiquitin-protein ligase subunit KPC2; GBDR1; Glialblastoma cell differentiation-related protein 1; Glialblastoma differentiation-related protein 1; Kip1 ubiquitination-promoting complex protein 2; kpc 2; Kpc2; OTTHUMP00000022563; Putative glialblastoma cell differentiation-related protein; RP11-432J22.3; UBA domain containing 1; UBA domain-containing protein 1; UBAC 1; UBAC1; Ubadc1; Ubiquitin associated domain containing 1; Ubiquitin-associated domain-containing protein 1;

Immunogens

Immunogen:
Uniprot:

Q9BSL1(UBAC1_HUMAN) >>Visit HPA database.

Gene(ID):
UBAC1(10422)
Expression:
Q9BSL1 UBAC1_HUMAN:

Ubiquitous.

Sequence:
MFVQEEKIFAGKVLRLHICASDGAEWLEEATEDTSVEKLKERCLKHCAHGSLEDPKSITHHKLIHAASERVLSDARTILEENIQDQDVLLLIKKRAPSPLPKMADVSAEEKKKQDQKAPDKEAILRATANLPSYNMDRAAVQTNMRDFQTELRKILVSLIEVAQKLLALNPDAVELFKKANAMLDEDEDERVDEAALRQLTEMGFPENRATKALQLNHMSVPQAMEWLIEHAEDPTIDTPLPGQAPPEAEGATAAASEAAAGASATDEEARDELTEIFKKIRRKREFRADARAVISLMEMGFDEKEVIDALRVNNNQQNAACEWLLGDRKPSPEELDKGIDPDSPLFQAILDNPVVQLGLTNPKTLLAFEDMLENPLNSTQWMNDPETGPVMLQISRIFQTLNRT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Xenopus
90
Horse
80
Dog
80
Zebrafish
80
Chicken
80
Pig
70
Bovine
70
Sheep
60
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BSL1 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K7 Ubiquitination
K12 Ubiquitination
K45 Ubiquitination
K56 Ubiquitination
K62 Ubiquitination
S68 Phosphorylation
K93 Ubiquitination
S98 Phosphorylation
K111 Ubiquitination
K117 Ubiquitination
K121 Ubiquitination
K154 Ubiquitination
K178 Acetylation
K178 Ubiquitination
K179 Ubiquitination
K279 Ubiquitination
S296 Phosphorylation
K305 Ubiquitination
K330 Ubiquitination
S332 Phosphorylation
K338 Ubiquitination
S344 Phosphorylation

Research Backgrounds

Function:

Non-catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Component of the KPC complex composed of RNF123/KPC1 and UBAC1/KPC2. Interacts with RNF123 via its N-terminal domain. Interacts with the proteasome via its N-terminal domain.

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