TMBIM1 Antibody | Affinity Biosciences

IF/ICC
Cites

1/3

FIGURE 6 Theexpressionofneuronalsignaling-transduction proteinsinhippocampusofDREratsat9weeksafterSE.

Product:	TMBIM1 Antibody
Catalog:	DF4580
Description:	Rabbit polyclonal antibody to TMBIM1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.:	35 KD; 35kD(Calculated).
Uniprot:	Q969X1
RRID:	AB_2836944

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(88%), Rabbit(100%), Dog(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

TMBIM1 Antibody detects endogenous levels of total TMBIM1.

RRID:

AB_2836944
Cite Format: Affinity Biosciences Cat# DF4580, RRID:AB_2836944.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

LFG3; MST100; MSTP100; PP1201; Protein lifeguard 3; Protein RECS1 homolog; RECS1; TMBI1_HUMAN; Tmbim1; Transmembrane BAX inhibitor motif containing 1; Transmembrane BAX inhibitor motif-containing protein 1;

Immunogens

Immunogen:

Uniprot:

Q969X1(LFG3_HUMAN) >>Visit HPA database.

Gene(ID):

TMBIM1(64114)

Sequence:

Q969X1 LFG3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSNPSAPPPYEDRNPLYPGPPPPGGYGQPSVLPGGYPAYPGYPQPGYGHPAGYPQPMPPTHPMPMNYGPGHGYDGEERAVSDSFGPGEWDDRKVRHTFIRKVYSIISVQLLITVAIIAIFTFVEPVSAFVRRNVAVYYVSYAVFVVTYLILACCQGPRRRFPWNIILLTLFTFAMGFMTGTISSMYQTKAVIIAMIITAVVSISVTIFCFQTKVDFTSCTGLFCVLGIVLLVTGIVTSIVLYFQYVYWLHMLYAALGAICFTLFLAYDTQLVLGNRKHTISPEDYITGALQIYTDIIYIFTFVLQLMGDRN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Dog

100

Xenopus

100

Rabbit

100

Horse

Sheep

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q969X1 As Substrate

Q969X1

Site	PTM Type	Source
S2	Phosphorylation	Uniprot
Y67	Phosphorylation	Uniprot
Y73	Phosphorylation	Uniprot
S81	Phosphorylation	Uniprot
S83	Phosphorylation	Uniprot
S127	Phosphorylation	Uniprot

Research Backgrounds

Q969X1_LFG3_HUMAN

Function:

Negatively regulates aortic matrix metalloproteinase-9 (MMP9) production and may play a protective role in vascular remodeling.

Subcellular Location:

Membrane>Multi-pass membrane protein. Lysosome membrane. Endosome membrane.

Family&Domains:

Belongs to the BI1 family. LFG subfamily.

References

1). miR‐485's anti‐drug resistant epilepsy effects by regulating SV2A/PSD‐95 and targeting ABCC1 and neuronal signaling‐transduction proteins in hippocampus of rats. Brain and Behavior, 2021 (PubMed: 34291586) [IF=3.1]

Application: WB Species: Rat Sample: hippocampus tissue

FIGURE 6 Theexpressionofneuronalsignaling-transduction proteinsinhippocampusofDREratsat9weeksafterSE.(a)Western blottingbands;(b)barographsofexpressionlevels.*p<.05versusthe

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For Research Use Only.
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TMBIM1 Antibody - #DF4580