EIF2B3 Antibody - #DF4578

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Product: EIF2B3 Antibody
Catalog: DF4578
Description: Rabbit polyclonal antibody to EIF2B3
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 50 KD; 50kD(Calculated).
Uniprot: Q9NR50
RRID: AB_2836942

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
EIF2B3 Antibody detects endogenous levels of total EIF2B3.
RRID:
AB_2836942
Cite Format: Affinity Biosciences Cat# DF4578, RRID:AB_2836942.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EI2BG_HUMAN; EIF 2B; eIF 2B GDP GTP exchange factor subunit gamma; eIF-2B GDP-GTP exchange factor subunit gamma; eIF2B-gamma; Eif2b3; EIF2Bgamma; Eukaryotic translation initiation factor 2B subunit 3 gamma; Eukaryotic translation initiation factor 2B, gamma; eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa; OTTHUMP00000010262; OTTHUMP00000010263; Translation initiation factor eIF-2B subunit gamma;

Immunogens

Immunogen:
Uniprot:

Q9NR50(EI2BG_HUMAN) >>Visit HPA database.

Gene(ID):
EIF2B3(8891)
Sequence:
MEFQAVVMAVGGGSRMTDLTSSIPKPLLPVGNKPLIWYPLNLLERVGFEEVIVVTTRDVQKALCAEFKMKMKPDIVCIPDDADMGTADSLRYIYPKLKTDVLVLSCDLITDVALHEVVDLFRAYDASLAMLMRKGQDSIEPVPGQKGKKKAVEQRDFIGVDSTGKRLLFMANEADLDEELVIKGSILQKHPRIRFHTGLVDAHLYCLKKYIVDFLMENGSITSIRSELIPYLVRKQFSSASSQQGQEEKEEDLKKKELKSLDIYSFIKEANTLNLAPYDACWNACRGDRWEDLSRSQVRCYVHIMKEGLCSRVSTLGLYMEANRQVPKLLSALCPEEPPVHSSAQIVSKHLVGVDSLIGPETQIGEKSSIKRSVIGSSCLIKDRVTITNCLLMNSVTVEEGSNIQGSVICNNAVIEKGADIKDCLIGSGQRIEAKAKRVNEVIVGNDQLMEI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
88
Sheep
88
Dog
88
Rabbit
88
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NR50 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K61 Ubiquitination
K68 Ubiquitination
K72 Ubiquitination
Y92 Phosphorylation
K96 Ubiquitination
K134 Methylation
K134 Ubiquitination
K146 Ubiquitination
K148 Methylation
K165 Ubiquitination
K189 Ubiquitination
S220 Phosphorylation
K235 Ubiquitination
K259 Ubiquitination
S260 Phosphorylation
Y264 Phosphorylation
S265 Phosphorylation
K268 Ubiquitination
S314 Phosphorylation
K328 Ubiquitination
K349 Ubiquitination
K367 Ubiquitination
K382 Ubiquitination
K422 Ubiquitination
S428 Phosphorylation

Research Backgrounds

Function:

Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.

Subunit Structure:

Complex of five different subunits; alpha, beta, gamma, delta and epsilon.

Family&Domains:

Belongs to the eIF-2B gamma/epsilon subunits family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

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