EIF2B4 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from COLO205 cells(heat-shock treatment), using EIF2B4 Antibody at 1/1000 dilution.

Product:	EIF2B4 Antibody
Catalog:	DF4577
Description:	Rabbit polyclonal antibody to EIF2B4
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	57 KD; 58kD(Calculated).
Uniprot:	Q9UI10
RRID:	AB_2836941

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(89%)

Clonality:

Polyclonal

Specificity:

EIF2B4 Antibody detects endogenous levels of total EIF2B4.

RRID:

AB_2836941
Cite Format: Affinity Biosciences Cat# DF4577, RRID:AB_2836941.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

EI2BD_HUMAN; EIF 2B; eIF 2B GDP GTP exchange factor subunit delta; eIF-2B GDP-GTP exchange factor subunit delta; Eif2b4; EIF2Bdelta; eukaryotic translation initiation factor 2B subunit 4; Translation initiation factor eIF-2B subunit delta;

Immunogens

Immunogen:

Uniprot:

Q9UI10(EI2BD_HUMAN) >>Visit HPA database.

Gene(ID):

EIF2B4(8890)

Sequence:

Q9UI10 EI2BD_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAVAVAVREDSGSGMKAELPPGPGAVGREMTKEEKLQLRKEKKQQKKKRKEEKGAEPETGSAVSAAQCQVGPTRELPESGIQLGTPREKVPAGRSKAELRAERRAKQEAERALKQARKGEQGGPPPKASPSTAGETPSGVKRLPEYPQVDDLLLRRLVKKPERQQVPTRKDYGSKVSLFSHLPQYSRQNSLTQFMSIPSSVIHPAMVRLGLQYSQGLVSGSNARCIALLRALQQVIQDYTTPPNEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFLNKEITSVGSSKREEEAKSELRAAIDRYVQEKIVLAAQAISRFAYQKISNGDVILVYGCSSLVSRILQEAWTEGRRFRVVVVDSRPWLEGRHTLRSLVHAGVPASYLLIPAASYVLPEVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCKRGEHVALANWQNHASLRLLNLVYDVTPPELVDLVITELGMIPCSSVPVVLRVKSSDQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UI10 As Substrate

Q9UI10

Site	PTM Type	Source
A2	Acetylation	Uniprot
S12	Phosphorylation	Uniprot
C69	S-Nitrosylation	Uniprot
T86	Phosphorylation	Uniprot
S130	Phosphorylation	Uniprot
T137	Phosphorylation	Uniprot
K142	Ubiquitination	Uniprot
Y147	Phosphorylation	Uniprot
K176	Ubiquitination	Uniprot
Y186	Phosphorylation	Uniprot
S187	Phosphorylation	Uniprot
S191	Phosphorylation	Uniprot
Y214	Phosphorylation	Uniprot
K282	Ubiquitination	Uniprot
K291	Ubiquitination	Uniprot
K297	Ubiquitination	Uniprot
S328	Phosphorylation	Uniprot
S340	Phosphorylation	Uniprot
R374	Methylation	Uniprot
S384	Phosphorylation	Uniprot
Y385	Phosphorylation	Uniprot
S399	Phosphorylation	Uniprot
K442	Ubiquitination	Uniprot
C465	S-Nitrosylation	Uniprot
K466	Acetylation	Uniprot
K466	Ubiquitination	Uniprot

Research Backgrounds

Q9UI10_EI2BD_HUMAN

Function:

Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.

Subunit Structure:

Complex of five different subunits; alpha, beta, gamma, delta and epsilon.

Family&Domains:

Belongs to the eIF-2B alpha/beta/delta subunits family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

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EIF2B4 Antibody - #DF4577