TAF3 Antibody - #DF4575

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Product: TAF3 Antibody
Catalog: DF4575
Description: Rabbit polyclonal antibody to TAF3
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 100 KD; 104kD(Calculated).
Uniprot: Q5VWG9
RRID: AB_2836939

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(88%), Bovine(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
TAF3 Antibody detects endogenous levels of total TAF3.
RRID:
AB_2836939
Cite Format: Affinity Biosciences Cat# DF4575, RRID:AB_2836939.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

140 kDa TATA box-binding protein-associated factor; Bip2 protein; RNA polymerase II transcription factor TAFII140; TAF(II)140; TAF140; TAF3; TAF3 RNA polymerase II TATA box binding protein (TBP)-associated factor; TAF3_HUMAN; TAFII-140; TAFII140; TAFII155 protein; TATA box binding protein associated factor 3; TBP-associated factor 3; Transcription initiation factor TFIID 140 kDa subunit; Transcription initiation factor TFIID subunit 3;

Immunogens

Immunogen:
Uniprot:

Q5VWG9(TAF3_HUMAN) >>Visit HPA database.

Gene(ID):
TAF3(83860)
Sequence:
MCESYSRSLLRVSVAQICQALGWDSVQLSACHLLTDVLQRYLQQLGRGCHRYSELYGRTDPILDDVGEAFQLMGVSLHELEDYIHNIEPVTFPHQIPSFPVSKNNVLQFPQPGSKDAEERKEYIPDYLPPIVSSQEEEEEEQVPTDGGTSAEAMQVPLEEDDELEEEEIINDENFLGKRPLDSPEAEELPAMKRPRLLSTKGDTLDVVLLEAREPLSSINTQKIPPMLSPVHVQDSTDLAPPSPEPPMLAPVAKSQMPTAKPLETKSFTPKTKTKTSSPGQKTKSPKTAQSPAMVGSPIRSPKTVSKEKKSPGRSKSPKSPKSPKVTTHIPQTPVRPETPNRTPSATLSEKISKETIQVKQIQTPPDAGKLNSENQPKKAVVADKTIEASIDAVIARACAEREPDPFEFSSGSESEGDIFTSPKRISGPECTTPKASTSANNFTKSGSTPLPLSGGTSSSDNSWTMDASIDEVVRKAKLGTPSNMPPNFPYISSPSVSPPTPEPLHKVYEEKTKLPSSVEVKKKLKKELKTKMKKKEKQRDREREKDKNKDKSKEKDKVKEKEKDKETGRETKYPWKEFLKEEEADPYKFKIKEFEDVDPKVKLKDGLVRKEKEKHKDKKKDREKGKKDKDKREKEKVKDKGREDKMKAPAPPLVLPPKELALPLFSPATASRVPAMLPSLLPVLPEKLFEEKEKVKEKEKKKDKKEKKKKKEKEKEKKEKEREKEKREREKREKEKEKHKHEKIKVEPVALAPSPVIPRLTLRVGAGQDKIVISKVVPAPEAKPAPSQNRPKTPPPAPAPAPGPMLVSPAPVPLPLLAQAAAGPALLPSPGPAASGASAKAPVRSVVTETVSTYVIRDEWGNQIWICPGCNKPDDGSPMIGCDDCDDWYHWPCVGIMTAPPEEMQWFCPKCANKKKDKKHKKRKHRAH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
88
Pig
0
Horse
0
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5VWG9 As Substrate

Site PTM Type Enzyme
Y52 Phosphorylation
Y56 Phosphorylation
S114 Phosphorylation
S183 Phosphorylation
S199 Phosphorylation
T200 Phosphorylation
T204 Phosphorylation
S229 Phosphorylation
S236 Phosphorylation
T237 Phosphorylation
S243 Phosphorylation
K261 Acetylation
T265 Phosphorylation
K266 Acetylation
S267 Phosphorylation
T269 Phosphorylation
T288 Phosphorylation
S291 Phosphorylation
S297 Phosphorylation
S301 Phosphorylation
S315 Phosphorylation
S317 Phosphorylation
S320 Phosphorylation
S323 Phosphorylation
T333 Phosphorylation
T339 Phosphorylation
T343 Phosphorylation
T347 Phosphorylation
S349 Phosphorylation
T364 Phosphorylation
K370 Ubiquitination
S373 Phosphorylation
S410 Phosphorylation
S411 Phosphorylation
S413 Phosphorylation
S415 Phosphorylation
S427 Phosphorylation
T432 Phosphorylation
T433 Phosphorylation
S483 Phosphorylation
Y491 Phosphorylation
S493 Phosphorylation
S494 Phosphorylation
S496 Phosphorylation
S498 Phosphorylation
T501 Phosphorylation
S517 Phosphorylation
S518 Phosphorylation
T568 Phosphorylation
T572 Phosphorylation
K601 Acetylation
K621 Acetylation
K625 Acetylation
K628 Acetylation
S667 Phosphorylation
K746 Sumoylation
S755 Phosphorylation
T762 Phosphorylation
K776 Acetylation
T794 Phosphorylation
S809 Phosphorylation

Research Backgrounds

Function:

Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Required in complex with TBPL2 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Belongs to the TFIID complex which is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Interacts with TAF10 via the histone fold. Interacts with TAF13, TBP, SAP130 and GCN5L2. Interacts with TBPL2.

Family&Domains:

The PHD-type zinc finger mediates binding to histone H3 methyllysine at position 4 (H3K4me3).

Belongs to the TAF3 family.

Research Fields

· Genetic Information Processing > Transcription > Basal transcription factors.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

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