Product: GTF3A Antibody
Catalog: DF4574
Description: Rabbit polyclonal antibody to GTF3A
Application: WB IF/ICC
Reactivity: Human
Mol.Wt.: 41 KD; 42kD(Calculated).
Uniprot: Q92664
RRID: AB_2836938

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
GTF3A Antibody detects endogenous levels of total GTF3A.
RRID:
AB_2836938
Cite Format: Affinity Biosciences Cat# DF4574, RRID:AB_2836938.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AP 2; AP2; Factor A; General transcription factor 3A; General transcription factor IIIA; GTF 3A; GTF3A; TF3A_HUMAN; TFIIIA; Transcription factor 3A; Transcription factor IIIA;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q92664 TF3A_HUMAN:

Ubiquitous.

Sequence:
MDPPAVVAESVSSLTIADAFIAAGESSAPTPPRPALPRRFICSFPDCSANYSKAWKLDAHLCKHTGERPFVCDYEGCGKAFIRDYHLSRHILTHTGEKPFVCAANGCDQKFNTKSNLKKHFERKHENQQKQYICSFEDCKKTFKKHQQLKIHQCQHTNEPLFKCTQEGCGKHFASPSKLKRHAKAHEGYVCQKGCSFVAKTWTELLKHVRETHKEEILCEVCRKTFKRKDYLKQHMKTHAPERDVCRCPREGCGRTYTTVFNLQSHILSFHEESRPFVCEHAGCGKTFAMKQSLTRHAVVHDPDKKKMKLKVKKSREKRSLASHLSGYIPPKRKQGQGLSLCQNGESPNCVEDKMLSTVAVLTLG

PTMs - Q92664 As Substrate

Site PTM Type Enzyme
Y51 Phosphorylation
S52 Phosphorylation
T65 Phosphorylation
Y74 Phosphorylation
Y85 Phosphorylation
T95 Phosphorylation
K114 Ubiquitination
K150 Ubiquitination
S175 Phosphorylation
K193 Ubiquitination
K227 Acetylation
K233 Acetylation
S347 Phosphorylation

Research Backgrounds

Function:

Involved in ribosomal large subunit biogenesis. Binds the approximately 50 base pairs internal control region (ICR) of 5S ribosomal RNA genes. It is required for their RNA polymerase III-dependent transcription and may also maintain the transcription of other genes. Also binds the transcribed 5S RNA's (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Restrictive clause

 

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