TACC3 Antibody - #DF4646

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Product: TACC3 Antibody
Catalog: DF4646
Description: Rabbit polyclonal antibody to TACC3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Rabbit, Dog, Chicken
Mol.Wt.: 80 KD; 90kD(Calculated).
Uniprot: Q9Y6A5
RRID: AB_2836937

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(91%), Horse(91%), Rabbit(91%), Dog(100%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
TACC3 Antibody detects endogenous levels of total TACC3.
RRID:
AB_2836937
Cite Format: Affinity Biosciences Cat# DF4646, RRID:AB_2836937.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ERIC 1; ERIC-1; ERIC1; MGC117382; MGC133242; OTTHUMP00000113796; TACC3; TACC3_HUMAN; Transforming acidic coiled coil containing protein 3; Transforming acidic coiled-coil-containing protein 3;

Immunogens

Immunogen:
Uniprot:

Q9Y6A5(TACC3_HUMAN) >>Visit HPA database.

Gene(ID):
TACC3(10460)
Sequence:
MSLQVLNDKNVSNEKNTENCDFLFSPPEVTGRSSVLRVSQKENVPPKNLAKAMKVTFQTPLRDPQTHRILSPSMASKLEAPFTQDDTLGLENSHPVWTQKENQQLIKEVDAKTTHGILQKPVEADTDLLGDASPAFGSGSSSESGPGALADLDCSSSSQSPGSSENQMVSPGKVSGSPEQAVEENLSSYSLDRRVTPASETLEDPCRTESQHKAETPHGAEEECKAETPHGAEEECRHGGVCAPAAVATSPPGAIPKEACGGAPLQGLPGEALGCPAGVGTPVPADGTQTLTCAHTSAPESTAPTNHLVAGRAMTLSPQEEVAAGQMASSSRSGPVKLEFDVSDGATSKRAPPPRRLGERSGLKPPLRKAAVRQQKAPQEVEEDDGRSGAGEDPPMPASRGSYHLDWDKMDDPNFIPFGGDTKSGCSEAQPPESPETRLGQPAAEQLHAGPATEEPGPCLSQQLHSASAEDTPVVQLAAETPTAESKERALNSASTSLPTSCPGSEPVPTHQQGQPALELKEESFRDPAEVLGTGAEVDYLEQFGTSSFKESALRKQSLYLKFDPLLRDSPGRPVPVATETSSMHGANETPSGRPREAKLVEFDFLGALDIPVPGPPPGVPAPGGPPLSTGPIVDLLQYSQKDLDAVVKATQEENRELRSRCEELHGKNLELGKIMDRFEEVVYQAMEEVQKQKELSKAEIQKVLKEKDQLTTDLNSMEKSFSDLFKRFEKQKEVIEGYRKNEESLKKCVEDYLARITQEGQRYQALKAHAEEKLQLANEEIAQVRSKAQAEALALQASLRKEQMRIQSLEKTVEQKTKENEELTRICDDLISKMEKI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Horse
91
Bovine
91
Rabbit
91
Chicken
82
Pig
75
Xenopus
73
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6A5 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K9 Ubiquitination
S12 Phosphorylation
S25 Phosphorylation
T30 Phosphorylation
R32 Methylation
S34 Phosphorylation
S39 Phosphorylation
K51 Methylation
K54 Methylation
T56 Phosphorylation
T59 Phosphorylation
T66 Phosphorylation
S71 Phosphorylation
S73 Phosphorylation
K100 Ubiquitination
S170 Phosphorylation
S175 Phosphorylation
S177 Phosphorylation
S187 Phosphorylation
T196 Phosphorylation
C206 S-Nitrosylation
T216 Phosphorylation
T228 Phosphorylation
T249 Phosphorylation
S250 Phosphorylation
T315 Phosphorylation
S317 Phosphorylation
S402 Phosphorylation
Y403 Phosphorylation
K423 Acetylation
C426 S-Nitrosylation
S434 Phosphorylation
T437 Phosphorylation
T481 Phosphorylation
T496 Phosphorylation
S505 Phosphorylation
S524 Phosphorylation
T534 Phosphorylation
Y540 Phosphorylation
K550 Ubiquitination
S552 Phosphorylation
S558 Phosphorylation O14965 (AURKA)
Y560 Phosphorylation
K562 Ubiquitination
S570 Phosphorylation
T590 Phosphorylation
S592 Phosphorylation
K649 Ubiquitination
K668 Ubiquitination
K674 Ubiquitination
Y684 Phosphorylation
K703 Ubiquitination
K720 Ubiquitination
S723 Phosphorylation
Y739 Phosphorylation
K748 Ubiquitination
K768 Ubiquitination
K788 Ubiquitination
S799 Phosphorylation
K819 Ubiquitination
K834 Ubiquitination

Research Backgrounds

Function:

Plays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors (By similarity). Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. May be involved in the control of cell growth and differentiation. May contribute to cancer.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle. Cytoplasm>Cytoskeleton>Spindle pole.
Note: In complex with CKAP5 localized to microtubule plus-ends in mitosis and interphase. In complex with CKAP5 and clathrin localized to inter-microtubule bridges in mitotic spindles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with microtubules. Interacts with CKAP5 independently of clathrin. Interacts with CKAP5 and clathrin forming the TACC3/ch-TOG/clathrin complex located at spindle inter-microtubules bridges; TACC3 (phosphorylated at Ser-558 by AURKA) and CLTC are proposed to form a composite microtubule interaction surface. Interacts with CCDC100/CEP120. The coiled coil C-terminal region interacts with AH receptor nuclear translocator protein (ARNT) and ARNT2 (By similarity). Interacts with GCN5L2 and PCAF.

Family&Domains:

Belongs to the TACC family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

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