ZP1 Antibody | Affinity Biosciences

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ZP1 Antibody - Western blot analysis of extracts from mouse kidney, using ZP1 Antibody.

ZP1 Antibody - DF4641 at 1/100 staining Rat kidney tissue by IHC-P.

Product:	ZP1 Antibody
Catalog:	DF4641
Description:	Rabbit polyclonal antibody to ZP1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	70 KD; 70kD(Calculated).
Uniprot:	P60852
RRID:	AB_2836932

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

ZP1 Antibody detects endogenous levels of total ZP1.

RRID:

AB_2836932
Cite Format: Affinity Biosciences Cat# DF4641, RRID:AB_2836932.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

MGC87693; Processed zona pellucida sperm-binding protein 1; zona pellucida glycoprotein 1 (sperm receptor); Zona pellucida glycoprotein 1; zona pellucida protein 1; Zp 1; Zp-1; Zp1; ZP1_HUMAN;

Immunogens

Immunogen:

Uniprot:

P60852(ZP1_HUMAN) >>Visit HPA database.

Gene(ID):

ZP1(22917)

Expression:

P60852 ZP1_HUMAN:

Expressed in oocytes (at protein level).

Sequence:

P60852 ZP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGGSATTWGYPVALLLLVATLGLGRWLQPDPGLPGLRHSYDCGIKGMQLLVFPRPGQTLRFKVVDEFGNRFDVNNCSICYHWVTSRPQEPAVFSADYRGCHVLEKDGRFHLRVFMEAVLPNGRVDVAQDATLICPKPDPSRTLDSQLAPPAMFSVSTPQTLSFLPTSGHTSQGSGHAFPSPLDPGHSSVHPTPALPSPGPGPTLATLAQPHWGTLEHWDVNKRDYIGTHLSQEQCQVASGHLPCIVRRTSKEACQQAGCCYDNTREVPCYYGNTATVQCFRDGYFVLVVSQEMALTHRITLANIHLAYAPTSCSPTQHTEAFVVFYFPLTHCGTTMQVAGDQLIYENWLVSGIHIQKGPQGSITRDSTFQLHVRCVFNASDFLPIQASIFPPPSPAPMTQPGPLRLELRIAKDETFSSYYGEDDYPIVRLLREPVHVEVRLLQRTDPNLVLLLHQCWGAPSANPFQQPQWPILSDGCPFKGDSYRTQMVALDGATPFQSHYQRFTVATFALLDSGSQRALRGLVYLFCSTSACHTSGLETCSTACSTGTTRQRRSSGHRNDTARPQDIVSSPGPVGFEDSYGQEPTLGPTDSNGNSSLRPLLWAVLLLPAVALVLGFGVFVGLSQTWAQKLWESNRQ

PTMs - P60852 As Substrate

P60852

Site	PTM Type	Source
T132	Phosphorylation	Uniprot
Y420	Phosphorylation	Uniprot
Y426	Phosphorylation	Uniprot

Research Backgrounds

P60852_ZP1_HUMAN

Function:

Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP1 ensures the structural integrity of the zona pellucida.

PTMs:

Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.

O-glycosylated.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix.
Note: Located in oocyte zona pellucida.

Cell membrane>Single-pass type I membrane protein.

Tissue Specificity:

Expressed in oocytes (at protein level).

Subunit Structure:

Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers (By similarity). Interacts with ZP3.

Family&Domains:

The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.

Belongs to the ZP domain family. ZPB subfamily.

Restrictive clause

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For Research Use Only.
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ZP1 Antibody - #DF4641