TCF4/12 Antibody - #DF4572

(1)  
Product: TCF4/12 Antibody
Catalog: DF4572
Description: Rabbit polyclonal antibody to TCF4/12
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 60 KD; 71kD,73kD(Calculated).
Uniprot: P15884 | Q99081
RRID: AB_2836923

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
TCF4/12 Antibody detects endogenous levels of total TCF4/12.
RRID:
AB_2836923
Cite Format: Affinity Biosciences Cat# DF4572, RRID:AB_2836923.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bHLHb19; Class B basic helix-loop-helix protein 19; E2 2; FECD3; Immunoglobulin transcription factor 2; ITF 2; ITF-2; ITF2; ITF2_HUMAN; MGC149723; MGC149724; PTHS; SEF 2; SEF-2; SEF2 1; SEF2 1A; SEF2 1B; SEF2 1D; SEF2; SL3 3 enhancer factor 2; SL3-3 enhancer factor 2; TCF 4; TCF-4; TCF4; Transcription factor 4; Transcription factor 4, isoform C; Transcription factor 4, isoform D; Transcription factor 4, isoform E; Transcription factor 4, isoform L; Transcription factor 4, isoform M; Transcription factor 4, isoform R; bHLHb20; Class B basic helix-loop-helix protein 20; DNA binding protein HTF4; DNA-binding protein HTF4; E box binding protein; E-box-binding protein; HEB; Helix loop helix transcription factor 4; HsT17266; HTF4; HTF4_HUMAN; TCF-12; Tcf12; Transcription factor 12; Transcription factor HTF-4; Transcription factor HTF4;

Immunogens

Immunogen:
Uniprot:

P15884(ITF2_HUMAN) >>Visit HPA database.

Q99081(HTF4_HUMAN) >>Visit HPA database.

Gene(ID):
TCF4(6925) | TCF12(6938)
Expression:
P15884 ITF2_HUMAN:

Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain.

Q99081 HTF4_HUMAN:

Expressed in several tissues and cell types including skeletal muscle, thymus, and a B-cell line.

Sequence:
MHHQQRMAALGTDKELSDLLDFSAMFSPPVSSGKNGPTSLASGHFTGSNVEDRSSSGSWGNGGHPSPSRNYGDGTPYDHMTSRDLGSHDNLSPPFVNSRIQSKTERGSYSSYGRESNLQGCHQQSLLGGDMDMGNPGTLSPTKPGSQYYQYSSNNPRRRPLHSSAMEVQTKKVRKVPPGLPSSVYAPSASTADYNRDSPGYPSSKPATSTFPSSFFMQDGHHSSDPWSSSSGMNQPGYAGMLGNSSHIPQSSSYCSLHPHERLSYPSHSSADINSSLPPMSTFHRSGTNHYSTSSCTPPANGTDSIMANRGSGAAGSSQTGDALGKALASIYSPDHTNNSFSSNPSTPVGSPPSLSAGTAVWSRNGGQASSSPNYEGPLHSLQSRIEDRLERLDDAIHVLRNHAVGPSTAMPGGHGDMHGIIGPSHNGAMGGLGSGYGTGLLSANRHSLMVGTHREDGVALRGSHSLLPNQVPVPQLPVQSATSPDLNPPQDPYRGMPPGLQGQSVSSGSSEIKSDDEGDENLQDTKSSEDKKLDDDKKDIKSITSNNDDEDLTPEQKAEREKERRMANNARERLRVRDINEAFKELGRMVQLHLKSDKPQTKLLILHQAVAVILSLEQQVRERNLNPKAACLKRREEEKVSSEPPPLSLAGPHPGMGDASNHMGQM

MNPQQQRMAAIGTDKELSDLLDFSAMFSPPVNSGKTRPTTLGSSQFSGSGIDERGGTTSWGTSGQPSPSYDSSRGFTDSPHYSDHLNDSRLGAHEGLSPTPFMNSNLMGKTSERGSFSLYSRDTGLPGCQSSLLRQDLGLGSPAQLSSSGKPGTAYYSFSATSSRRRPLHDSAALDPLQAKKVRKVPPGLPSSVYAPSPNSDDFNRESPSYPSPKPPTSMFASTFFMQDGTHNSSDLWSSSNGMSQPGFGGILGTSTSHMSQSSSYGNLHSHDRLSYPPHSVSPTDINTSLPPMSSFHRGSTSSSPYVAASHTPPINGSDSILGTRGNAAGSSQTGDALGKALASIYSPDHTSSSFPSNPSTPVGSPSPLTGTSQWPRPGGQAPSSPSYENSLHSLQSRMEDRLDRLDDAIHVLRNHAVGPSTSLPAGHSDIHSLLGPSHNAPIGSLNSNYGGSSLVASSRSASMVGTHREDSVSLNGNHSVLSSTVTTSSTDLNHKTQENYRGGLQSQSGTVVTTEIKTENKEKDENLHEPPSSDDMKSDDESSQKDIKVSSRGRTSSTNEDEDLNPEQKIEREKERRMANNARERLRVRDINEAFKELGRMCQLHLKSEKPQTKLLILHQAVAVILSLEQQVRERNLNPKAACLKRREEEKVSAVSAEPPTTLPGTHPGLSETTNPMGHM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P15884/Q99081 As Substrate

Site PTM Type Enzyme
S54 Phosphorylation
S55 Phosphorylation
S56 Phosphorylation
S66 Phosphorylation
S68 Phosphorylation
S87 Phosphorylation
S92 Phosphorylation
R106 Methylation
Y149 Phosphorylation
T297 Phosphorylation
S318 Phosphorylation
S372 Phosphorylation
Y375 Phosphorylation
S484 Phosphorylation
S515 Phosphorylation
T554 Phosphorylation
K585 Ubiquitination
K629 Ubiquitination
Site PTM Type Enzyme
T13 Phosphorylation
K35 Ubiquitination
T39 Phosphorylation
S43 Phosphorylation
S44 Phosphorylation
S47 Phosphorylation
S49 Phosphorylation
S59 Phosphorylation
T62 Phosphorylation
S67 Phosphorylation
S72 Phosphorylation
T77 Phosphorylation
S79 Phosphorylation
Y82 Phosphorylation
S83 Phosphorylation
S89 Phosphorylation
S98 Phosphorylation
T100 Phosphorylation
K110 Ubiquitination
T111 Phosphorylation
R114 Methylation
S116 Phosphorylation
Y120 Phosphorylation
S132 Phosphorylation
S142 Phosphorylation
K151 Ubiquitination
Y156 Phosphorylation
Y157 Phosphorylation
K181 Ubiquitination
K185 Ubiquitination
S198 Phosphorylation
S201 Phosphorylation
S263 Phosphorylation
S283 Phosphorylation
T285 Phosphorylation
S301 Phosphorylation
T302 Phosphorylation
S303 Phosphorylation
S304 Phosphorylation
S305 Phosphorylation
Y307 Phosphorylation
S311 Phosphorylation
T313 Phosphorylation
S321 Phosphorylation
T325 Phosphorylation
S333 Phosphorylation
T335 Phosphorylation
S385 Phosphorylation
S386 Phosphorylation
S388 Phosphorylation
S392 Phosphorylation
S395 Phosphorylation
S462 Phosphorylation
S464 Phosphorylation
S473 Phosphorylation
T488 Phosphorylation
S490 Phosphorylation
R503 Methylation
S508 Phosphorylation
K519 Sumoylation
S540 Phosphorylation
S545 Phosphorylation
K550 Sumoylation
K550 Ubiquitination
S552 Phosphorylation
S553 Phosphorylation
T557 Phosphorylation
S558 Phosphorylation
S559 Phosphorylation
T560 Phosphorylation
K571 Ubiquitination
K598 Ubiquitination
K609 Ubiquitination
S610 Phosphorylation
K612 Ubiquitination
K642 Ubiquitination
K653 Ubiquitination

Research Backgrounds

Function:

Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain.

Subunit Structure:

Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or heterooligomers with myogenin. Interacts with HIVEP2. Interacts with NEUROD2 (By similarity). Interacts with AGBL1. Interacts with BHLHA9.

Family&Domains:

the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Function:

Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3').

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in several tissues and cell types including skeletal muscle, thymus, and a B-cell line.

Subunit Structure:

Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or heterooligomers with myogenin, E12 and ITF2 proteins. Interacts with PTF1A. Interacts with NEUROD2 (By similarity). Interacts with RUNX1T1. Interacts with AML1-MTG8/ETO (via nervy homology region 2 in oligomerized form). Interacts with BHLHA9.

Family&Domains:

the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.