CCT8 Antibody - #DF4561

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Product: CCT8 Antibody
Catalog: DF4561
Description: Rabbit polyclonal antibody to CCT8
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 59 KD; 60kD(Calculated).
Uniprot: P50990
RRID: AB_2836912

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(91%), Chicken(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
CCT8 Antibody detects endogenous levels of total CCT8.
RRID:
AB_2836912
Cite Format: Affinity Biosciences Cat# DF4561, RRID:AB_2836912.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C21orf112; CCT 8; CCT theta; CCT8; CCTQ; Chaperonin containing TCP1 subunit 8 (theta); Chaperonin subunit 8 (Theta) (Predicted), isoform CRA_a; Chaperonin subunit 8 (theta); Chromosome 21 open reading frame 112; D21S246; KIAA0002; PRED71; Renal carcinoma antigen NY REN 15; T complex protein 1 subunit theta; T complex protein 1 theta subunit; TCP 1 theta; Tcpq;

Immunogens

Immunogen:
Uniprot:

P50990(TCPQ_HUMAN) >>Visit HPA database.

Gene(ID):
CCT8(10694)
Sequence:
MALHVPKAPGFAQMLKEGAKHFSGLEEAVYRNIQACKELAQTTRTAYGPNGMNKMVINHLEKLFVTNDAATILRELEVQHPAAKMIVMASHMQEQEVGDGTNFVLVFAGALLELAEELLRIGLSVSEVIEGYEIACRKAHEILPNLVCCSAKNLRDIDEVSSLLRTSIMSKQYGNEVFLAKLIAQACVSIFPDSGHFNVDNIRVCKILGSGISSSSVLHGMVFKKETEGDVTSVKDAKIAVYSCPFDGMITETKGTVLIKTAEELMNFSKGEENLMDAQVKAIADTGANVVVTGGKVADMALHYANKYNIMLVRLNSKWDLRRLCKTVGATALPRLTPPVLEEMGHCDSVYLSEVGDTQVVVFKHEKEDGAISTIVLRGSTDNLMDDIERAVDDGVNTFKVLTRDKRLVPGGGATEIELAKQITSYGETCPGLEQYAIKKFAEAFEAIPRALAENSGVKANEVISKLYAVHQEGNKNVGLDIEAEVPAVKDMLEAGILDTYLGKYWAIKLATNAAVTVLRVDQIIMAKPAGGPKPPSGKKDWDDDQND

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Chicken
100
Rabbit
100
Dog
91
Xenopus
91
Zebrafish
91
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P50990 As Substrate

Site PTM Type Enzyme
K7 Acetylation
K7 Ubiquitination
K16 Acetylation
K16 Ubiquitination
K20 Acetylation
K20 Ubiquitination
S23 Phosphorylation
Y30 Phosphorylation
K37 Ubiquitination
K54 Ubiquitination
K62 Ubiquitination
K138 Ubiquitination
C148 S-Nitrosylation
C149 S-Nitrosylation
S150 Phosphorylation
K152 Acetylation
K152 Ubiquitination
S162 Phosphorylation
K171 Ubiquitination
Y173 Phosphorylation
S210 Phosphorylation
S213 Phosphorylation
S216 Phosphorylation
K224 Ubiquitination
K225 Acetylation
K225 Ubiquitination
T227 Phosphorylation
T232 Phosphorylation
S233 Phosphorylation
K235 Ubiquitination
T251 Phosphorylation
K254 Ubiquitination
T256 Phosphorylation
K260 Ubiquitination
T261 Phosphorylation
S269 Phosphorylation
K270 Ubiquitination
T286 Phosphorylation
T293 Phosphorylation
K296 Acetylation
K296 Ubiquitination
Y304 Phosphorylation
K307 Ubiquitination
S317 Phosphorylation
K318 Acetylation
K318 Ubiquitination
K326 Acetylation
K326 Ubiquitination
T327 Phosphorylation
T331 Phosphorylation
K367 Acetylation
K367 Ubiquitination
R378 Methylation
S380 Phosphorylation
T381 Phosphorylation
K400 Acetylation
K400 Methylation
K400 Ubiquitination
T415 Phosphorylation
K421 Ubiquitination
Y426 Phosphorylation
T429 Phosphorylation
C430 S-Nitrosylation
Y436 Phosphorylation
K439 Acetylation
K439 Ubiquitination
K440 Ubiquitination
K459 Sumoylation
K459 Ubiquitination
K466 Acetylation
K466 Ubiquitination
Y501 Phosphorylation
Y505 Phosphorylation
T512 Phosphorylation
T517 Phosphorylation
K528 Acetylation
K528 Methylation
K528 Ubiquitination
K534 Acetylation
K534 Sumoylation
K534 Ubiquitination
S537 Phosphorylation
K539 Acetylation
K539 Ubiquitination
K540 Acetylation
K540 Ubiquitination

Research Backgrounds

Function:

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin (Probable).

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Cilium basal body.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the chaperonin-containing T-complex (TRiC), a heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG. Interacts with DNAAF4 (By similarity).

Family&Domains:

Belongs to the TCP-1 chaperonin family.

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