CCT7 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from HepG2 cells(heat-shock treatment), using CCT7 Antibody.

Product:	CCT7 Antibody
Catalog:	DF4560
Description:	Rabbit polyclonal antibody to CCT7
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.:	59 KD; 59kD(Calculated).
Uniprot:	Q99832
RRID:	AB_2836911

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(91%), Xenopus(91%)

Clonality:

Polyclonal

Specificity:

CCT7 Antibody detects endogenous levels of total CCT7.

RRID:

AB_2836911
Cite Format: Affinity Biosciences Cat# DF4560, RRID:AB_2836911.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CCT ETA; CCT-eta; CCT7; CCTETA; Ccth; Chaperonin containing t complex polypeptide 1 eta subunit; Chaperonin containing TCP1 subunit 7 (eta); Chaperonin containing TCP1 subunit 7; HIV 1 Nef interacting protein; HIV-1 Nef-interacting protein; Nip7 1; T complex protein 1 eta subunit; T-complex protein 1 subunit eta, N-terminally processed; TCP-1-eta; TCP1eta; TCPH_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q99832(TCPH_HUMAN) >>Visit HPA database.

Gene(ID):

CCT7(10574)

Sequence:

Q99832 TCPH_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MMPTPVILLKEGTDSSQGIPQLVSNISACQVIAEAVRTTLGPRGMDKLIVDGRGKATISNDGATILKLLDVVHPAAKTLVDIAKSQDAEVGDGTTSVTLLAAEFLKQVKPYVEEGLHPQIIIRAFRTATQLAVNKIKEIAVTVKKADKVEQRKLLEKCAMTALSSKLISQQKAFFAKMVVDAVMMLDDLLQLKMIGIKKVQGGALEDSQLVAGVAFKKTFSYAGFEMQPKKYHNPKIALLNVELELKAEKDNAEIRVHTVEDYQAIVDAEWNILYDKLEKIHHSGAKVVLSKLPIGDVATQYFADRDMFCAGRVPEEDLKRTMMACGGSIQTSVNALSADVLGRCQVFEETQIGGERYNFFTGCPKAKTCTFILRGGAEQFMEETERSLHDAIMIVRRAIKNDSVVAGGGAIEMELSKYLRDYSRTIPGKQQLLIGAYAKALEIIPRQLCDNAGFDATNILNKLRARHAQGGTWYGVDINNEDIADNFEAFVWEPAMVRINALTAASEAACLIVSVDETIKNPRSTVDAPTAAGRGRGRGRPH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99832 As Substrate

Q99832

Site	PTM Type	Source
M1	Acetylation	Uniprot
T4	Phosphorylation	Uniprot
T13	Phosphorylation	Uniprot
S16	Phosphorylation	Uniprot
S24	Phosphorylation	Uniprot
S27	Phosphorylation	Uniprot
R43	Methylation	Uniprot
K47	Ubiquitination	Uniprot
R53	Methylation	Uniprot
K55	Ubiquitination	Uniprot
T57	Phosphorylation	Uniprot
S59	Phosphorylation	Uniprot
T64	Phosphorylation	Uniprot
K67	Acetylation	Uniprot
K67	Ubiquitination	Uniprot
K77	Ubiquitination	Uniprot
K84	Ubiquitination	Uniprot
K106	Ubiquitination	Uniprot
K109	Ubiquitination	Uniprot
T129	Phosphorylation	Uniprot
K135	Ubiquitination	Uniprot
K137	Ubiquitination	Uniprot
K144	Ubiquitination	Uniprot
K157	Ubiquitination	Uniprot
K166	Ubiquitination	Uniprot
K172	Acetylation	Uniprot
K172	Ubiquitination	Uniprot
K199	Ubiquitination	Uniprot
K217	Acetylation	Uniprot
K217	Ubiquitination	Uniprot
K218	Acetylation	Uniprot
K218	Ubiquitination	Uniprot
S221	Phosphorylation	Uniprot
Y222	Phosphorylation	Uniprot
K230	Ubiquitination	Uniprot
K231	Acetylation	Uniprot
K247	Ubiquitination	Uniprot
K250	Acetylation	Uniprot
K250	Ubiquitination	Uniprot
T259	Phosphorylation	Uniprot
Y263	Phosphorylation	Uniprot
Y275	Phosphorylation	Uniprot
K277	Ubiquitination	Uniprot
K280	Ubiquitination	Uniprot
K287	Acetylation	Uniprot
K287	Ubiquitination	Uniprot
K292	Acetylation	Uniprot
K292	Ubiquitination	Uniprot
T300	Phosphorylation	Uniprot
Y302	Phosphorylation	Uniprot
R306	Methylation	Uniprot
K320	Acetylation	Uniprot
K320	Ubiquitination	Uniprot
T322	Phosphorylation	Uniprot
T332	Phosphorylation	Uniprot
S338	Phosphorylation	Uniprot
C364	S-Nitrosylation	Uniprot
K366	Acetylation	Uniprot
K366	Ubiquitination	Uniprot
K368	Ubiquitination	Uniprot
C370	S-Nitrosylation	Uniprot
T371	Phosphorylation	Uniprot
R375	Methylation	Uniprot
K401	Ubiquitination	Uniprot
K418	Ubiquitination	Uniprot
K430	Methylation	Uniprot
K430	Ubiquitination	Uniprot
K440	Ubiquitination	Uniprot
C450	S-Nitrosylation	Uniprot
K463	Acetylation	Uniprot
K463	Ubiquitination	Uniprot
T504	Phosphorylation	Uniprot
S507	Phosphorylation	Uniprot
K521	Ubiquitination	Uniprot
R524	Methylation	Uniprot
T526	Phosphorylation	Uniprot
T531	Phosphorylation	Uniprot
R535	Methylation	Uniprot
R537	Methylation	Uniprot
R539	Methylation	Uniprot
R541	Methylation	Uniprot

Research Backgrounds

Q99832_TCPH_HUMAN

Function:

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. The TRiC complex plays a role in the folding of actin and tubulin (Probable).

Subcellular Location:

Cytoplasm.

Subunit Structure:

Component of the chaperonin-containing T-complex (TRiC), a heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG.

Family&Domains:

Belongs to the TCP-1 chaperonin family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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CCT7 Antibody - #DF4560