CCT5 Antibody - #DF4559

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Product: CCT5 Antibody
Catalog: DF4559
Description: Rabbit polyclonal antibody to CCT5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 67 KD; 60kD(Calculated).
Uniprot: P48643
RRID: AB_2836910

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(92%), Dog(100%), Chicken(92%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
CCT5 Antibody detects endogenous levels of total CCT5.
RRID:
AB_2836910
Cite Format: Affinity Biosciences Cat# DF4559, RRID:AB_2836910.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CCT 5; CCT epsilon; CCT5; CCTE; CCTepsilon; Chaperonin containing T-complex polypeptide 1, subunit 5; Chaperonin containing TCP 1 epsilon; Chaperonin containing TCP 1 subunit 5; Chaperonin containing TCP 1 subunit 5 epsilon; Chaperonin containing TCP1 epsilon; Chaperonin containing TCP1 subunit 5; Chaperonin containing TCP1 subunit 5 epsilon; KIAA0098; T complex protein 1 epsilon subunit; T complex protein 1 subunit epsilon; TCP 1 epsilon; TCP1epsilon; TCPE;

Immunogens

Immunogen:
Uniprot:

P48643(TCPE_HUMAN) >>Visit HPA database.

Gene(ID):
CCT5(22948)
Sequence:
MASMGTLAFDEYGRPFLIIKDQDRKSRLMGLEALKSHIMAAKAVANTMRTSLGPNGLDKMMVDKDGDVTVTNDGATILSMMDVDHQIAKLMVELSKSQDDEIGDGTTGVVVLAGALLEEAEQLLDRGIHPIRIADGYEQAARVAIEHLDKISDSVLVDIKDTEPLIQTAKTTLGSKVVNSCHRQMAEIAVNAVLTVADMERRDVDFELIKVEGKVGGRLEDTKLIKGVIVDKDFSHPQMPKKVEDAKIAILTCPFEPPKPKTKHKLDVTSVEDYKALQKYEKEKFEEMIQQIKETGANLAICQWGFDDEANHLLLQNNLPAVRWVGGPEIELIAIATGGRIVPRFSELTAEKLGFAGLVQEISFGTTKDKMLVIEQCKNSRAVTIFIRGGNKMIIEEAKRSLHDALCVIRNLIRDNRVVYGGGAAEISCALAVSQEADKCPTLEQYAMRAFADALEVIPMALSENSGMNPIQTMTEVRARQVKEMNPALGIDCLHKGTNDMKQQHVIETLIGKKQQISLATQMVRMILKIDDIRKPGESEE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Bovine
92
Sheep
92
Xenopus
92
Zebrafish
92
Chicken
92
Rabbit
92
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P48643 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S3 Phosphorylation
Y12 Phosphorylation
K20 Ubiquitination
K25 Ubiquitination
S26 Phosphorylation
R27 Methylation
K35 Ubiquitination
K42 Sumoylation
K42 Ubiquitination
R49 Methylation
S51 Phosphorylation
K59 Acetylation
K59 Ubiquitination
K64 Acetylation
K64 Ubiquitination
R132 Methylation
K150 Ubiquitination
S154 Phosphorylation
K160 Acetylation
K160 Sumoylation
K160 Ubiquitination
T168 Phosphorylation
K170 Ubiquitination
K176 Sumoylation
K176 Ubiquitination
C181 S-Nitrosylation
K210 Sumoylation
K210 Ubiquitination
K214 Ubiquitination
K223 Acetylation
K223 Ubiquitination
K226 Sumoylation
K226 Ubiquitination
K232 Acetylation
K232 Ubiquitination
S235 Phosphorylation
K242 Acetylation
K247 Ubiquitination
K259 Ubiquitination
K261 Acetylation
K261 Ubiquitination
K263 Acetylation
K263 Ubiquitination
K265 Acetylation
K265 Sumoylation
K265 Ubiquitination
S270 Phosphorylation
Y274 Phosphorylation
K275 Acetylation
K275 Ubiquitination
K279 Acetylation
K279 Sumoylation
K279 Ubiquitination
K282 Acetylation
K282 Ubiquitination
K284 Ubiquitination
S346 Phosphorylation
K368 Ubiquitination
K370 Ubiquitination
C377 S-Nitrosylation
K378 Ubiquitination
K392 Ubiquitination
K399 Acetylation
K399 Ubiquitination
C407 S-Nitrosylation
K439 Ubiquitination
S463 Phosphorylation
S466 Phosphorylation
T473 Phosphorylation
K483 Ubiquitination
K496 Acetylation
K496 Ubiquitination
K502 Sumoylation
K502 Ubiquitination
T509 Phosphorylation
K513 Ubiquitination
K514 Ubiquitination
S518 Phosphorylation
T521 Phosphorylation
K529 Ubiquitination
R534 Methylation
K535 Ubiquitination
S539 Phosphorylation

Research Backgrounds

Function:

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin (Probable).

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the chaperonin-containing T-complex (TRiC), a heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG. Interacts with DNAAF4 (By similarity).

Family&Domains:

Belongs to the TCP-1 chaperonin family.

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