CD1E Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/3

Western blot analysis of extracts from Mouse spleen, using CD1E Antibody.

Fig 3 Ambient PM exposure leads to hepatic steatosis by impairing hepatic lipid metabolism.

Product:	CD1E Antibody
Catalog:	DF4558
Description:	Rabbit polyclonal antibody to CD1E
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Dog
Mol.Wt.:	36 KD, 54 kDa; 44kD(Calculated).
Uniprot:	P15812
RRID:	AB_2836909

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(80%), Dog(90%)

Clonality:

Polyclonal

Specificity:

CD1E Antibody detects endogenous levels of total CD1E.

RRID:

AB_2836909
Cite Format: Affinity Biosciences Cat# DF4558, RRID:AB_2836909.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD_antigen=CD1e; CD1A; CD1e; CD1e antigen; CD1E antigen, e polypeptide; CD1e molecule; CD1E_HUMAN; Differentiation antigen CD1-alpha-3; hCD1e; Leukocyte differentiation antigen; R2; R2G1; sCD1e; soluble; T-cell surface glycoprotein CD1e; T-cell surface glycoprotein CD1e, membrane-associated; T-cell surface glycoprotein CD1e, soluble;

Immunogens

Immunogen:

Uniprot:

P15812(CD1E_HUMAN) >>Visit HPA database.

Gene(ID):

CD1E(913)

Expression:

P15812 CD1E_HUMAN:

Expressed on cortical thymocytes, dendritic cells, Langerhans cells, on certain T-cell leukemias, and in various other tissues.

Sequence:

P15812 CD1E_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLLLFLLFEGLCCPGENTAAPQALQSYHLAAEEQLSFRMLQTSSFANHSWAHSEGSGWLGDLQTHGWDTVLGTIRFLKPWSHGNFSKQELKNLQSLFQLYFHSFIQIVQASAGQFQLEYPFEIQILAGCRMNAPQIFLNMAYQGSDFLSFQGISWEPSPGAGIRAQNICKVLNRYLDIKEILQSLLGHTCPRFLAGLMEAGESELKRKVKPEAWLSCGPSPGPGRLQLVCHVSGFYPKPVWVMWMRGEQEQRGTQRGDVLPNADETWYLRATLDVAAGEAAGLSCRVKHSSLGGHDLIIHWGGYSIFLILICLTVIVTLVILVVVDSRLKKQSSNKNILSPHTPSPVFLMGANTQDTKNSRHQFCLAQVSWIKNRVLKKWKTRLNQLW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Pig

Bovine

Sheep

Horse

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P15812 As Substrate

P15812

Site	PTM Type	Source
K78	Ubiquitination	Uniprot
K87	Ubiquitination	Uniprot
K170	Ubiquitination	Uniprot
K179	Ubiquitination	Uniprot
K206	Ubiquitination	Uniprot
K208	Ubiquitination	Uniprot
K210	Ubiquitination	Uniprot
K336	Ubiquitination	Uniprot
T357	Phosphorylation	Uniprot
K358	Ubiquitination	Uniprot
S370	Phosphorylation	Uniprot
K373	Ubiquitination	Uniprot

Research Backgrounds

P15812_CD1E_HUMAN

Function:

T-cell surface glycoprotein CD1e, soluble binds diacetylated lipids, including phosphatidyl inositides and diacylated sulfoglycolipids, and is required for the presentation of glycolipid antigens on the cell surface. The membrane-associated form is not active.

PTMs:

Mono-ubiquitinated.

Proteolytically cleaved in late endosomes to yield a soluble form.

Subcellular Location:

Golgi apparatus membrane>Single-pass type I membrane protein. Early endosome. Late endosome.
Note: Predominantly localized in the trans-Golgi network in immature dendritic cells, and as a cleaved, soluble protein in the lysosome lumen of mature dendritic cells.

Lysosome lumen.

Tissue Specificity:

Expressed on cortical thymocytes, dendritic cells, Langerhans cells, on certain T-cell leukemias, and in various other tissues.

Subunit Structure:

Heterodimer with B2M (beta-2-microglobulin). The association with B2M appears to be facilitated by the presence of the propeptide.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)

References

1). Combined effects of ambient particulate matter exposure and a high-fat diet on oxidative stress and steatohepatitis in mice. PLoS One, 2019 (PubMed: 30921449) [IF=3.7]

Application: WB Species: Mice Sample: liver tissues

Fig 3 Ambient PM exposure leads to hepatic steatosis by impairing hepatic lipid metabolism. (A) Oil Red O staining observation of liver (×200, scale bars = 100 μm). (B) H&E staining observation of liver (×200, scale bars = 50 μm). (C) The volume density of quantitation of hepatic steatosis (n = 5). (D) The genes expression involved in fatty acid β-oxidation in liver (n = 5). (E) The mRNA expression of genes involved in lipogenesis and FXR in liver (n = 5). (F) Bands of PPARα, PPARγ, ACOX1, FAS, SREBP-1c, SCD1. (G) Protein expression of SREBP-1c (n = 3). (H) Protein expression of FAS (n = 3). (I) Protein expression of SCD1 (n = 3). (J) Protein expression of PPARγ (n = 3). (K) Protein expression of PPARα (n = 3). (L) Protein expression of ACOX1 (n = 3). (M) Bands of LC3-I and LC3-II. (N) The ratio of LC3-II and LC3-I (n = 3). Data are expressed as the mean ± SD. *, P<0.05 and **, P<0.01 compared with the STD-FA group. #, P<0.05 and ##, P<0.01 compared with the STD-PM group. Δ, P<0.05 and ΔΔ, P<0.01 compared with the STD-PM group. Diet** indicates the HFD. PM** indicates PM exposure. *, P<0.05 and **, P<0.01. Diet×PM indicates the interaction effect between HFD and PM exposure.

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CD1E Antibody - #DF4558