CD1E Antibody - #DF4558

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Product: CD1E Antibody
Catalog: DF4558
Description: Rabbit polyclonal antibody to CD1E
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Dog
Mol.Wt.: 36 KD, 54 kDa; 44kD(Calculated).
Uniprot: P15812
RRID: AB_2836909

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(80%), Dog(90%)
Clonality:
Polyclonal
Specificity:
CD1E Antibody detects endogenous levels of total CD1E.
RRID:
AB_2836909
Cite Format: Affinity Biosciences Cat# DF4558, RRID:AB_2836909.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD_antigen=CD1e; CD1A; CD1e; CD1e antigen; CD1E antigen, e polypeptide; CD1e molecule; CD1E_HUMAN; Differentiation antigen CD1-alpha-3; hCD1e; Leukocyte differentiation antigen; R2; R2G1; sCD1e; soluble; T-cell surface glycoprotein CD1e; T-cell surface glycoprotein CD1e, membrane-associated; T-cell surface glycoprotein CD1e, soluble;

Immunogens

Immunogen:
Uniprot:

P15812(CD1E_HUMAN) >>Visit HPA database.

Gene(ID):
CD1E(913)
Expression:
P15812 CD1E_HUMAN:

Expressed on cortical thymocytes, dendritic cells, Langerhans cells, on certain T-cell leukemias, and in various other tissues.

Sequence:
MLLLFLLFEGLCCPGENTAAPQALQSYHLAAEEQLSFRMLQTSSFANHSWAHSEGSGWLGDLQTHGWDTVLGTIRFLKPWSHGNFSKQELKNLQSLFQLYFHSFIQIVQASAGQFQLEYPFEIQILAGCRMNAPQIFLNMAYQGSDFLSFQGISWEPSPGAGIRAQNICKVLNRYLDIKEILQSLLGHTCPRFLAGLMEAGESELKRKVKPEAWLSCGPSPGPGRLQLVCHVSGFYPKPVWVMWMRGEQEQRGTQRGDVLPNADETWYLRATLDVAAGEAAGLSCRVKHSSLGGHDLIIHWGGYSIFLILICLTVIVTLVILVVVDSRLKKQSSNKNILSPHTPSPVFLMGANTQDTKNSRHQFCLAQVSWIKNRVLKKWKTRLNQLW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
90
Pig
80
Bovine
50
Sheep
50
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P15812 As Substrate

Site PTM Type Enzyme
K78 Ubiquitination
K87 Ubiquitination
K170 Ubiquitination
K179 Ubiquitination
K206 Ubiquitination
K208 Ubiquitination
K210 Ubiquitination
K336 Ubiquitination
T357 Phosphorylation
K358 Ubiquitination
S370 Phosphorylation
K373 Ubiquitination

Research Backgrounds

Function:

T-cell surface glycoprotein CD1e, soluble binds diacetylated lipids, including phosphatidyl inositides and diacylated sulfoglycolipids, and is required for the presentation of glycolipid antigens on the cell surface. The membrane-associated form is not active.

PTMs:

Mono-ubiquitinated.

Proteolytically cleaved in late endosomes to yield a soluble form.

Subcellular Location:

Golgi apparatus membrane>Single-pass type I membrane protein. Early endosome. Late endosome.
Note: Predominantly localized in the trans-Golgi network in immature dendritic cells, and as a cleaved, soluble protein in the lysosome lumen of mature dendritic cells.

Lysosome lumen.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed on cortical thymocytes, dendritic cells, Langerhans cells, on certain T-cell leukemias, and in various other tissues.

Subunit Structure:

Heterodimer with B2M (beta-2-microglobulin). The association with B2M appears to be facilitated by the presence of the propeptide.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

References

1). Combined effects of ambient particulate matter exposure and a high-fat diet on oxidative stress and steatohepatitis in mice. PLoS One (PubMed: 30921449) [IF=3.7]

Application: WB    Species: Mice    Sample: liver tissues

Fig 3 Ambient PM exposure leads to hepatic steatosis by impairing hepatic lipid metabolism. (A) Oil Red O staining observation of liver (×200, scale bars = 100 μm). (B) H&E staining observation of liver (×200, scale bars = 50 μm). (C) The volume density of quantitation of hepatic steatosis (n = 5). (D) The genes expression involved in fatty acid β-oxidation in liver (n = 5). (E) The mRNA expression of genes involved in lipogenesis and FXR in liver (n = 5). (F) Bands of PPARα, PPARγ, ACOX1, FAS, SREBP-1c, SCD1. (G) Protein expression of SREBP-1c (n = 3). (H) Protein expression of FAS (n = 3). (I) Protein expression of SCD1 (n = 3). (J) Protein expression of PPARγ (n = 3). (K) Protein expression of PPARα (n = 3). (L) Protein expression of ACOX1 (n = 3). (M) Bands of LC3-I and LC3-II. (N) The ratio of LC3-II and LC3-I (n = 3). Data are expressed as the mean ± SD. *, P<0.05 and **, P<0.01 compared with the STD-FA group. #, P<0.05 and ##, P<0.01 compared with the STD-PM group. Δ, P<0.05 and ΔΔ, P<0.01 compared with the STD-PM group. Diet** indicates the HFD. PM** indicates PM exposure. *, P<0.05 and **, P<0.01. Diet×PM indicates the interaction effect between HFD and PM exposure.

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