Product: SYT11 Antibody
Catalog: DF4552
Description: Rabbit polyclonal antibody to SYT11
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 48 KD; 48kD(Calculated).
Uniprot: Q9BT88
RRID: AB_2836903

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
SYT11 Antibody detects endogenous levels of total SYT11.
RRID:
AB_2836903
Cite Format: Affinity Biosciences Cat# DF4552, RRID:AB_2836903.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp781D015; KIAA0080; MGC10881; MGC17226; Synaptotagmin 11; Synaptotagmin 12; Synaptotagmin XI; Synaptotagmin-11; Synaptotagmin11; Synaptotagmin12; SynaptotagminXI; SYT 11; SYT 12; Syt XI; Syt11; SYT11_HUMAN; SYT12; SytXI;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAEITNIRPSFDVSPVVAGLIGASVLVVCVSVTVFVWSCCHQQAEKKQKNPPYKFIHMLKGISIYPETLSNKKKIIKVRRDKDGPGREGGRRNLLVDAAEAGLLSRDKDPRGPSSGSCIDQLPIKMDYGEELRSPITSLTPGESKTTSPSSPEEDVMLGSLTFSVDYNFPKKALVVTIQEAHGLPVMDDQTQGSDPYIKMTILPDKRHRVKTRVLRKTLDPVFDETFTFYGIPYSQLQDLVLHFLVLSFDRFSRDDVIGEVMVPLAGVDPSTGKVQLTRDIIKRNIQKCISRGELQVSLSYQPVAQRMTVVVLKARHLPKMDITGLSGNPYVKVNVYYGRKRIAKKKTHVKKCTLNPIFNESFIYDIPTDLLPDISIEFLVIDFDRTTKNEVVGRLILGAHSVTASGAEHWREVCESPRKPVAKWHSLSEY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
91
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BT88 As Substrate

Site PTM Type Enzyme
K54 Ubiquitination
S63 Phosphorylation
Y65 Phosphorylation
Y128 Phosphorylation
T137 Phosphorylation
Y197 Phosphorylation
Y301 Phosphorylation
T309 Phosphorylation
Y331 Phosphorylation
Y337 Phosphorylation
Y338 Phosphorylation
T387 Phosphorylation

Research Backgrounds

Function:

Synaptotagmin family member involved in vesicular and membrane trafficking which does not bind Ca(2+). Inhibits clathrin-mediated and bulk endocytosis, functions to ensure precision in vesicle retrieval. Plays an important role in dopamine transmission by regulating endocytosis and the vesicle-recycling process. Essential component of a neuronal vesicular trafficking pathway that differs from the synaptic vesicle trafficking pathway but is crucial for development and synaptic plasticity. In macrophages and microglia, inhibits the conventional cytokine secretion, of at least IL6 and TNF, and phagocytosis. In astrocytes, regulates lysosome exocytosis, mechanism required for the repair of injured astrocyte cell membrane (By similarity). Required for the ATP13A2-mediated regulation of the autophagy-lysosome pathway.

PTMs:

Ubiquitinated, at least by PRKN, and targeted to the proteasome complex for degradation. Ubiquitination is inhibited by ATP13A2.

Subcellular Location:

Cytoplasmic vesicle membrane>Single-pass membrane protein. Perikaryon. Golgi apparatus>trans-Golgi network membrane>Single-pass membrane protein. Recycling endosome membrane>Single-pass membrane protein. Lysosome membrane>Single-pass membrane protein. Cytoplasmic vesicle>Phagosome. Cell projection>Axon. Cell projection>Dendrite. Cell junction>Synapse>Postsynaptic density. Recycling endosome membrane>Single-pass membrane protein. Cytoplasmic vesicle>Clathrin-coated vesicle membrane>Single-pass membrane protein. Perikaryon.
Note: Localized in vesicles that travels in axonal and dendritic shafts in both anterograde and retrograde directions. In macrophages and microglia, recruited in phagosomes at early stages of phagocytosis (By similarity). Found in the core of the Lewy bodies in the brain of sporadic Parkinson disease patients (PubMed:12925569).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Can also form heterodimers. Interacts with PRKN. Interacts (via C2 2 domain) with AGO2 and SND1; the interaction with SND1 is direct. Interacts with KIF1A; the interaction increases in presence of calcium (By similarity).

Family&Domains:

The second C2 domain/C2B is required for the inhibitory role in both clathrin-mediated and bulk endocytosis. The transmembrane domain and the first C2 domain/C2A are critical for the inhibitory role in clathrin-mediated endocytosis or bulk endocytosis, respectively.

Unlike in other synaptotagmin family members, the first C2 domain/C2A does not bind Ca(2+) neither mediates Ca(2+)-dependent phospholipid binding. An aspartate-to-serine substitution in this domain inactivates Ca(2+)/phospho-lipid binding.

Belongs to the synaptotagmin family.

Restrictive clause

 

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