SF3B3 Antibody - #DF4539

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Product: SF3B3 Antibody
Catalog: DF4539
Description: Rabbit polyclonal antibody to SF3B3
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 136 KD; 136kD(Calculated).
Uniprot: Q15393
RRID: AB_2836890

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
SF3B3 Antibody detects endogenous levels of total SF3B3.
RRID:
AB_2836890
Cite Format: Affinity Biosciences Cat# DF4539, RRID:AB_2836890.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

130 kDa Sin3-associated polypeptide; FLJ12761; Histone deacetylase complex subunit SAP130; KIAA0017; mSin3A associated protein 130; Pre mRNA splicing factor SF3b 130 kDa subunit; RSE1; SAP 130; Sap130; SF3b 130; SF3b130; SF3B3; Sin3-associated polypeptide p130; Sin3A associated protein 130kDa; Sin3A associated protein; SP130_HUMAN; Spliceosome associated protein 130; Splicing factor 3b subunit 3 130kDa; Splicing factor 3b subunit 3; STAF 130; STAF130;

Immunogens

Immunogen:
Uniprot:

Q15393(SF3B3_HUMAN) >>Visit HPA database.

Gene(ID):
SF3B3(23450)
Sequence:
MFLYNLTLQRATGISFAIHGNFSGTKQQEIVVSRGKILELLRPDPNTGKVHTLLTVEVFGVIRSLMAFRLTGGTKDYIVVGSDSGRIVILEYQPSKNMFEKIHQETFGKSGCRRIVPGQFLAVDPKGRAVMISAIEKQKLVYILNRDAAARLTISSPLEAHKANTLVYHVVGVDVGFENPMFACLEMDYEEADNDPTGEAAANTQQTLTFYELDLGLNHVVRKYSEPLEEHGNFLITVPGGSDGPSGVLICSENYITYKNFGDQPDIRCPIPRRRNDLDDPERGMIFVCSATHKTKSMFFFLAQTEQGDIFKITLETDEDMVTEIRLKYFDTVPVAAAMCVLKTGFLFVASEFGNHYLYQIAHLGDDDEEPEFSSAMPLEEGDTFFFQPRPLKNLVLVDELDSLSPILFCQIADLANEDTPQLYVACGRGPRSSLRVLRHGLEVSEMAVSELPGNPNAVWTVRRHIEDEFDAYIIVSFVNATLVLSIGETVEEVTDSGFLGTTPTLSCSLLGDDALVQVYPDGIRHIRADKRVNEWKTPGKKTIVKCAVNQRQVVIALTGGELVYFEMDPSGQLNEYTERKEMSADVVCMSLANVPPGEQRSRFLAVGLVDNTVRIISLDPSDCLQPLSMQALPAQPESLCIVEMGGTEKQDELGERGSIGFLYLNIGLQNGVLLRTVLDPVTGDLSDTRTRYLGSRPVKLFRVRMQGQEAVLAMSSRSWLSYSYQSRFHLTPLSYETLEFASGFASEQCPEGIVAISTNTLRILALEKLGAVFNQVAFPLQYTPRKFVIHPESNNLIIIETDHNAYTEATKAQRKQQMAEEMVEAAGEDERELAAEMAAAFLNENLPESIFGAPKAGNGQWASVIRVMNPIQGNTLDLVQLEQNEAAFSVAVCRFSNTGEDWYVLVGVAKDLILNPRSVAGGFVYTYKLVNNGEKLEFLHKTPVEEVPAAIAPFQGRVLIGVGKLLRVYDLGKKKLLRKCENKHIANYISGIQTIGHRVIVSDVQESFIWVRYKRNENQLIIFADDTYPRWVTTASLLDYDTVAGADKFGNICVVRLPPNTNDEVDEDPTGNKALWDRGLLNGASQKAEVIMNYHVGETVLSLQKTTLIPGGSESLVYTTLSGGIGILVPFTSHEDHDFFQHVEMHLRSEHPPLCGRDHLSFRSYYFPVKNVIDGDLCEQFNSMEPNKQKNVSEELDRTPPEVSKKLEDIRTRYAF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Pig
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15393 As Substrate

Site PTM Type Enzyme
K26 Ubiquitination
K36 Ubiquitination
S64 Phosphorylation
T71 Phosphorylation
K75 Ubiquitination
Y77 Phosphorylation
S82 Phosphorylation
S84 Phosphorylation
Y92 Phosphorylation
S95 Phosphorylation
K96 Ubiquitination
K101 Ubiquitination
K109 Acetylation
K109 Ubiquitination
K126 Acetylation
K126 Ubiquitination
S133 Phosphorylation
K137 Ubiquitination
K139 Ubiquitination
T153 Phosphorylation
S155 Phosphorylation
S156 Phosphorylation
R268 Methylation
T314 Phosphorylation
K328 Ubiquitination
S434 Phosphorylation
K537 Ubiquitination
K546 Ubiquitination
S687 Phosphorylation
S696 Phosphorylation
K700 Ubiquitination
K769 Ubiquitination
T784 Phosphorylation
K787 Ubiquitination
Y807 Phosphorylation
K812 Ubiquitination
K816 Ubiquitination
Y904 Phosphorylation
Y926 Phosphorylation
K936 Ubiquitination
K942 Ubiquitination
K965 Ubiquitination
R968 Methylation
K974 Ubiquitination
Y989 Phosphorylation
Y1029 Phosphorylation
Y1041 Phosphorylation
T1043 Phosphorylation
K1049 Ubiquitination
K1074 Ubiquitination
R1079 Methylation
K1088 Ubiquitination
R1164 Methylation
K1171 Ubiquitination
K1189 Ubiquitination
K1191 Ubiquitination
S1194 Phosphorylation
T1200 Phosphorylation
K1206 Acetylation
K1206 Ubiquitination

Research Backgrounds

Function:

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Identified in the spliceosome A complex; remains associated with the spliceosome throughout the splicing process. Component of the spliceosome B complex. Identified in the spliceosome C complex. Identified in the spliceosome E complex. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Component of splicing factor SF3B complex which is composed of at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A and DDX42. SF3B associates with the splicing factor SF3A and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Interaction between SF3B3 and SF3B1 is tighter than the interaction between SF3B3 and SF3B2. Within the SF3B complex interacts directly with SF3B1 (via HEAT domain), SF3B5 and PHF5A. The SF3B complex composed of SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6 and PHF5A interacts with U2AF2. Associates with the STAGA transcription coactivator-HAT complex. Interacts with SUPT3H. Interacts with TAF3.

Family&Domains:

The core of the protein consists of three beta-propeller domains.

Belongs to the RSE1 family.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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