SNX4 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of extracts from EC304 cells(heat-shock treatment), using SNX4 Antibody at 1/1000 dilution.

DF4538 at 1/100 staining rat brain tissue by IHC-P.

Product:	SNX4 Antibody
Catalog:	DF4538
Description:	Rabbit polyclonal antibody to SNX4
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	60 KD; 52kD(Calculated).
Uniprot:	O95219
RRID:	AB_2836889

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Zebrafish(82%), Bovine(82%), Horse(91%), Sheep(82%), Rabbit(91%), Dog(91%), Chicken(91%), Xenopus(82%)

Clonality:

Polyclonal

Specificity:

SNX4 Antibody detects endogenous levels of total SNX4.

RRID:

AB_2836889
Cite Format: Affinity Biosciences Cat# DF4538, RRID:AB_2836889.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ATG24B; SNX 4; snx4; SNX4_HUMAN; Sorting nexin 4; Sorting nexin-4;

Immunogens

Immunogen:

Uniprot:

O95219(SNX4_HUMAN) >>Visit HPA database.

Gene(ID):

SNX4(8723)

Sequence:

O95219 SNX4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEQAPPDPERQLQPAPLEPLGSPDAGLGAAVGKEAEGAGEESSGVDTMTHNNFWLKKIEISVSEAEKRTGRNAMNMQETYTAYLIETRSVEHTDGQSVLTDSLWRRYSEFELLRSYLLVYYPHIVVPPLPEKRAEFVWHKLSADNMDPDFVERRRIGLENFLLRIASHPILCRDKIFYLFLTQEGNWKETVNETGFQLKADSRLKALNATFRVKNPDKRFTDLKHYSDELQSVISHLLRVRARVADRLYGVYKVHGNYGRVFSEWSAIEKEMGDGLQSAGHHMDVYASSIDDILEDEEHYADQLKEYLFYAEALRAVCRKHELMQYDLEMAAQDLASKKQQCEELVTGTVRTFSLKGMTTKLFGQETPEQREARIKVLEEQINEGEQQLKSKNLEGREFVKNAWADIERFKEQKNRDLKEALISYAVMQISMCKKGIQVWTNAKECFSKM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Dog

Chicken

Rabbit

Bovine

Sheep

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95219 As Substrate

O95219

Site	PTM Type	Source
M1	Acetylation	Uniprot
S22	Phosphorylation	Uniprot
K33	Ubiquitination	Uniprot
K56	Ubiquitination	Uniprot
K57	Ubiquitination	Uniprot
K67	Ubiquitination	Uniprot
S89	Phosphorylation	Uniprot
S97	Phosphorylation	Uniprot
S108	Phosphorylation	Uniprot
K132	Ubiquitination	Uniprot
K140	Ubiquitination	Uniprot
C172	S-Nitrosylation	Uniprot
K199	Ubiquitination	Uniprot
K205	Methylation	Uniprot
K205	Ubiquitination	Uniprot
T210	Phosphorylation	Uniprot
K224	Ubiquitination	Uniprot
R247	Methylation	Uniprot
K253	Ubiquitination	Uniprot
K320	Ubiquitination	Uniprot
K338	Ubiquitination	Uniprot
K339	Ubiquitination	Uniprot
T347	Phosphorylation	Uniprot
S354	Phosphorylation	Uniprot
K356	Methylation	Uniprot
K356	Ubiquitination	Uniprot
K361	Ubiquitination	Uniprot
T367	Phosphorylation	Uniprot
K376	Ubiquitination	Uniprot
K390	Ubiquitination	Uniprot
K401	Ubiquitination	Uniprot
K419	Acetylation	Uniprot
K444	Ubiquitination	Uniprot
K449	Ubiquitination	Uniprot

Research Backgrounds

O95219_SNX4_HUMAN

Function:

Involved in the regulation of endocytosis and in several stages of intracellular trafficking. Plays a role in recycling endocytosed transferrin receptor and prevent its degradation.

Subcellular Location:

Early endosome. Early endosome membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Also detected on a juxtanuclear endocytic recycling compartment (ERC).

Subunit Structure:

Interacts with WWC1/KIBRA. Identified in a complex with WWC1/KIBRA and dynein components DYNLL1 and DYNC1I2. Interacts with BIN1.

Family&Domains:

The PX domain binds phosphatidylinositol 3-phosphate which is necessary for peripheral membrane localization.

Belongs to the sorting nexin family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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SNX4 Antibody - #DF4538