SLCO1B1 Antibody | Affinity Biosciences

WB
IF/ICC
Cites

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SLCO1B1 Antibody - Western blot analysis of extracts from mouse liver, using SLCO1B1 Antibody.

SLCO1B1 Antibody - DF4534 staining HepG2 cells by IF/ICC.

SLCO1B1 Antibody - FIGURE 1 | Effects of berberine on OATP1B1 expression in HepG2 cells.

SLCO1B1 Antibody - Figure 10 (A) Expression of the seven genes in the risk signature in the high-risk and low-risk groups.

SLCO1B1 Antibody - Fig.

Product:	SLCO1B1 Antibody
Catalog:	DF4534
Description:	Rabbit polyclonal antibody to SLCO1B1
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	76 KD; 76kD(Calculated).
Uniprot:	Q9Y6L6
RRID:	AB_2819223

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

SLCO1B1 Antibody detects endogenous levels of total SLCO1B1.

RRID:

AB_2819223
Cite Format: Affinity Biosciences Cat# DF4534, RRID:AB_2819223.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Liver-specific organic anion transporter 1; LST-1; LST1; OATP-2; OATP-C; OATP1B1; OATPC; SLC21A6; SLCO1B1; SO1B1_HUMAN; Sodium-independent organic anion-transporting polypeptide 2; Solute carrier family 21 member 6; Solute carrier organic anion transporter family member 1B1;

Immunogens

Immunogen:

Uniprot:

Q9Y6L6(SO1B1_HUMAN) >>Visit HPA database.

Gene(ID):

SLCO1B1(10599)

Expression:

Q9Y6L6 SO1B1_HUMAN:

Highly expressed in liver, at the basolateral membranes of centrilobular hepatocytes. Not detected in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocyte.

Sequence:

Q9Y6L6 SO1B1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDQNQHLNKTAEAQPSENKKTRYCNGLKMFLAALSLSFIAKTLGAIIMKSSIIHIERRFEISSSLVGFIDGSFEIGNLLVIVFVSYFGSKLHRPKLIGIGCFIMGIGGVLTALPHFFMGYYRYSKETNINSSENSTSTLSTCLINQILSLNRASPEIVGKGCLKESGSYMWIYVFMGNMLRGIGETPIVPLGLSYIDDFAKEGHSSLYLGILNAIAMIGPIIGFTLGSLFSKMYVDIGYVDLSTIRITPTDSRWVGAWWLNFLVSGLFSIISSIPFFFLPQTPNKPQKERKASLSLHVLETNDEKDQTANLTNQGKNITKNVTGFFQSFKSILTNPLYVMFVLLTLLQVSSYIGAFTYVFKYVEQQYGQPSSKANILLGVITIPIFASGMFLGGYIIKKFKLNTVGIAKFSCFTAVMSLSFYLLYFFILCENKSVAGLTMTYDGNNPVTSHRDVPLSYCNSDCNCDESQWEPVCGNNGITYISPCLAGCKSSSGNKKPIVFYNCSCLEVTGLQNRNYSAHLGECPRDDACTRKFYFFVAIQVLNLFFSALGGTSHVMLIVKIVQPELKSLALGFHSMVIRALGGILAPIYFGALIDTTCIKWSTNNCGTRGSCRTYNSTSFSRVYLGLSSMLRVSSLVLYIILIYAMKKKYQEKDINASENGSVMDEANLESLNKNKHFVPSAGADSETHC

PTMs - Q9Y6L6 As Substrate

Q9Y6L6

Site	PTM Type	Source
N134	N-Glycosylation	Uniprot
S293	Phosphorylation	Uniprot
S295	Phosphorylation	Uniprot
T301	Phosphorylation	Uniprot
T312	Phosphorylation	Uniprot
T319	Phosphorylation	Uniprot
T323	Phosphorylation	Uniprot
S328	Phosphorylation	Uniprot
Y367	Phosphorylation	Uniprot
S420	Phosphorylation	Uniprot
Y422	Phosphorylation	Uniprot
Y425	Phosphorylation	Uniprot
N503	N-Glycosylation	Uniprot
N516	N-Glycosylation	Uniprot
Y625	Phosphorylation	Uniprot
S636	Phosphorylation	Uniprot
Y645	Phosphorylation	Uniprot
S659	Phosphorylation	Uniprot
S663	Phosphorylation	Uniprot
S672	Phosphorylation	Uniprot
S682	Phosphorylation	Uniprot
S687	Phosphorylation	Uniprot
T689	Phosphorylation	Uniprot

Research Backgrounds

Q9Y6L6_SO1B1_HUMAN

Function:

Mediates the Na(+)-independent uptake of organic anions such as pravastatin, taurocholate, methotrexate, dehydroepiandrosterone sulfate, 17-beta-glucuronosyl estradiol, estrone sulfate, prostaglandin E2, thromboxane B2, leukotriene C3, leukotriene E4, thyroxine and triiodothyronine. Involved in the clearance of bile acids and organic anions from the liver.

Subcellular Location:

Basolateral cell membrane>Multi-pass membrane protein.
Note: Detected in basolateral membranes of hepatocytes.

Tissue Specificity:

Family&Domains:

Belongs to the organo anion transporter (TC 2.A.60) family.

References

1). Mn (II) Complex of Lipophilic Group-Modified Ethylenediaminetetraacetic Acid (EDTA) as a New Hepatobiliary MRI Contrast Agent. JOURNAL OF MEDICINAL CHEMISTRY, 2021 (PubMed: 34152137) [IF=6.8]

2). Based on disulfidptosis-related glycolytic genes to construct a signature for predicting prognosis and immune infiltration analysis of hepatocellular carcinoma. Frontiers in Immunology, 2023 (PubMed: 37680641) [IF=5.7]

Application: IHC Species: Human Sample: liver tissues

Figure 10 (A) Expression of the seven genes in the risk signature in the high-risk and low-risk groups. (B) Correlation of the seven genes in the train group. (C) Correlation of the seven genes in the test group. (D) Correlation between the level of immune cell infiltration and the seven genes in the risk model. (E) Differential expression of SLCO1B1 in HCC tissues and normal tissues. The red part represents HCC patient samples, and the gray part represents normal patient tissue samples. (F) The result of qRT-PCR. (G) Normal and cancer images of SLCO1B1 expression in liver tissues (100× and 400×) detected by IHC staining. (H) IHC score for all samples. **** represents P

3). Berberine Promotes OATP1B1 Expression and Rosuvastatin Uptake by Inducing Nuclear Translocation of FXR and LXRα. Frontiers in Pharmacology, 2020 (PubMed: 32292349) [IF=5.6]

Application: WB Species: human Sample: HepG2 cells

FIGURE 1 | Effects of berberine on OATP1B1 expression in HepG2 cells. (C, D) OATP1B1 protein levels were determined by Western blotting in HepG2 cells after treatment with increasing concentrations of berberine (5, 10, 25, and 50mM) for 24 h or treatment with 25 mM berberine at a series of time points (6, 12, 24, and 48 h). Data are represent as mean ± SD from triplicate independent experiments after being normalized to GAPDH, and 0.1% dimethylsulfoxide (DMSO) were used as the negative control. ***P < 0.001 compared to the control.

4). Pregnane X receptor promotes liver enlargement in mice through the spatial induction of hepatocyte hypertrophy and proliferation. Chemico-Biological Interactions, 2022 (PubMed: 36030841) [IF=4.7]

5). Dexamethasone-Induced Liver Enlargement Is Related to PXR/YAP Activation and Lipid Accumulation but Not Hepatocyte Proliferation. Drug Metabolism and Disposition, 2020 (PubMed: 32561593) [IF=4.4]

Application: WB Species: Mouse Sample:

Fig. 2. Dex activates mPXR and hPXR. (A and B) Western blot and quantification of mPXR downstream proteins from vehicle- or Dex-treated mice livers. Data are expressed as mean ± S.D. (n = 3). *P < 0.05; **P < 0.01 compared with the vehicle group. (C) Dual-luciferase reporter gene assay was used to determine the effect of RIF or Dex on hPXR activation in HEK293T cells. Data are expressed as mean ± S.D. (n = 5). **P < 0.01; ***P < 0.001; ****P < 0.0001 compared with the control group.

6). An integrated strategy combining network toxicology and feature-based molecular networking for exploring hepatotoxic constituents and mechanism of Epimedii Folium-induced hepatotoxicity in vitro. Food and Chemical Toxicology, 2023 (PubMed: 37080529) [IF=3.9]

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SLCO1B1 Antibody - #DF4534