SLC6A3 Antibody - #DF4529

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Product: SLC6A3 Antibody
Catalog: DF4529
Description: Rabbit polyclonal antibody to SLC6A3
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 68 KD; 68kD(Calculated).
Uniprot: Q01959
RRID: AB_2836880

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Dog(91%)
Clonality:
Polyclonal
Specificity:
SLC6A3 Antibody detects endogenous levels of total SLC6A3.
RRID:
AB_2836880
Cite Format: Affinity Biosciences Cat# DF4529, RRID:AB_2836880.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DA transporter; DAT 1; DAT; DAT1; Dopamine transporter 1; Dopamine transporter; PKDYS; SC6A3_HUMAN; SLC6A3; Sodium dependent dopamine transporter; Sodium-dependent dopamine transporter; Solute carrier family 6 (neurotransmitter transporter dopamine), member 3; Solute carrier family 6 (neurotransmitter transporter), member 3; Solute carrier family 6 member 3; Variable number tandem repeat (VNTR);

Immunogens

Immunogen:
Uniprot:

Q01959(SC6A3_HUMAN) >>Visit HPA database.

Gene(ID):
SLC6A3(6531)
Expression:
Q01959 SC6A3_HUMAN:

Highly expressed in substantia nigra.

Sequence:
MSKSKCSVGLMSSVVAPAKEPNAVGPKEVELILVKEQNGVQLTSSTLTNPRQSPVEAQDRETWGKKIDFLLSVIGFAVDLANVWRFPYLCYKNGGGAFLVPYLLFMVIAGMPLFYMELALGQFNREGAAGVWKICPILKGVGFTVILISLYVGFFYNVIIAWALHYLFSSFTTELPWIHCNNSWNSPNCSDAHPGDSSGDSSGLNDTFGTTPAAEYFERGVLHLHQSHGIDDLGPPRWQLTACLVLVIVLLYFSLWKGVKTSGKVVWITATMPYVVLTALLLRGVTLPGAIDGIRAYLSVDFYRLCEASVWIDAATQVCFSLGVGFGVLIAFSSYNKFTNNCYRDAIVTTSINSLTSFSSGFVVFSFLGYMAQKHSVPIGDVAKDGPGLIFIIYPEAIATLPLSSAWAVVFFIMLLTLGIDSAMGGMESVITGLIDEFQLLHRHRELFTLFIVLATFLLSLFCVTNGGIYVFTLLDHFAAGTSILFGVLIEAIGVAWFYGVGQFSDDIQQMTGQRPSLYWRLCWKLVSPCFLLFVVVVSIVTFRPPHYGAYIFPDWANALGWVIATSSMAMVPIYAAYKFCSLPGSFREKLAYAIAPEKDRELVDRGEVRQFTLRHWLKV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Horse
91
Bovine
91
Sheep
91
Dog
91
Xenopus
67
Zebrafish
64
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q01959 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
S4 Phosphorylation
K5 Ubiquitination
S7 Phosphorylation P17612 (PRKACA)
S12 Phosphorylation
S13 Phosphorylation
S53 Phosphorylation P28482 (MAPK1)
T62 Phosphorylation
T278 Phosphorylation
T286 Phosphorylation
Y593 Phosphorylation
T613 Phosphorylation

Research Backgrounds

Function:

Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in substantia nigra.

Subunit Structure:

Homooligomer; disulfide-linked (Ref.14). Interacts with PRKCABP and TGFB1I1. Interacts (via N-terminus) with SYNGR3 (via N-terminus). Interacts with SLC18A2. Interacts with TOR1A (ATP-bound); TOR1A regulates SLC6A3 subcellular location (By similarity). Interacts with alpha-synuclein/SNCA.

Family&Domains:

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.

Research Fields

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Organismal Systems > Nervous system > Dopaminergic synapse.

References

1). Integrative multilevel exploration of the mechanism by which Er-Zhi-Wan alleviates the Parkinson's disease (PD)-like phenotype in the MPTP-induced PD mouse model. Biomedicine & Pharmacotherapy, 2023 (PubMed: 37348406) [IF=7.5]

Application: WB    Species: Mouse    Sample:

Fig. 3. EZW reduces the dopaminergic neuron damage in MPTP-PD mice. (A) The expression of TH, α-syn, and DAT in the striatum of each group was measured by Western blot analysis (n = 3 per group). (B) Relative densities of TH, α-syn, DAT, and β-actin were measured in different groups using densitometric analysis of Western blots. TH, α-syn, and DAT proteins were normalized to β-actin, and were expressed as the fold difference relative to the control group (Data represent the mean ± SD, n = 3. * P 

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