SLC6A1 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from rat brain, using SLC6A1 Antibody.

Product:	SLC6A1 Antibody
Catalog:	DF4510
Description:	Rabbit polyclonal antibody to SLC6A1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	67 KD; 67kD(Calculated).
Uniprot:	P30531
RRID:	AB_2836861

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

SLC6A1 Antibody detects endogenous levels of total SLC6A1.

RRID:

AB_2836861
Cite Format: Affinity Biosciences Cat# DF4510, RRID:AB_2836861.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

GABATHG; GABATR; GABT 1; GABT1; GAT-1; GAT1; SC6A1_HUMAN; Slc6a1; Sodium and chloride dependent GABA transporter 1; Sodium- and chloride-dependent GABA transporter 1; Solute carrier family 6 (neurotransmitter transporter GABA) member 1; Solute carrier family 6 member 1;

Immunogens

Immunogen:

Uniprot:

P30531(SC6A1_HUMAN) >>Visit HPA database.

Gene(ID):

SLC6A1(6529)

Sequence:

P30531 SC6A1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MATNGSKVADGQISTEVSEAPVANDKPKTLVVKVQKKAADLPDRDTWKGRFDFLMSCVGYAIGLGNVWRFPYLCGKNGGGAFLIPYFLTLIFAGVPLFLLECSLGQYTSIGGLGVWKLAPMFKGVGLAAAVLSFWLNIYYIVIISWAIYYLYNSFTTTLPWKQCDNPWNTDRCFSNYSMVNTTNMTSAVVEFWERNMHQMTDGLDKPGQIRWPLAITLAIAWILVYFCIWKGVGWTGKVVYFSATYPYIMLIILFFRGVTLPGAKEGILFYITPNFRKLSDSEVWLDAATQIFFSYGLGLGSLIALGSYNSFHNNVYRDSIIVCCINSCTSMFAGFVIFSIVGFMAHVTKRSIADVAASGPGLAFLAYPEAVTQLPISPLWAILFFSMLLMLGIDSQFCTVEGFITALVDEYPRLLRNRRELFIAAVCIISYLIGLSNITQGGIYVFKLFDYYSASGMSLLFLVFFECVSISWFYGVNRFYDNIQEMVGSRPCIWWKLCWSFFTPIIVAGVFIFSAVQMTPLTMGNYVFPKWGQGVGWLMALSSMVLIPGYMAYMFLTLKGSLKQRIQVMVQPSEDIVRPENGPEQPQAGSSTSKEAYI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30531 As Substrate

P30531

Site	PTM Type	Source
T3	Phosphorylation	Uniprot
S6	Phosphorylation	Uniprot
Y107	Phosphorylation	Uniprot
Y317	Phosphorylation	Uniprot

Research Backgrounds

P30531_SC6A1_HUMAN

Function:

Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Membrane>Multi-pass membrane protein. Cell junction>Synapse>Presynapse.
Note: Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons (By similarity).

Subunit Structure:

Interacts with MPP5.

Family&Domains:

The PDZ domain-binding motif is involved in the interaction with MPP5.

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily.

Research Fields

· Organismal Systems > Nervous system > GABAergic synapse.

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SLC6A1 Antibody - #DF4510