SIRPB1 Antibody | Affinity Biosciences

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Cites

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Western blot analysis of extracts from HuvEc cells using SIRPB1 antibody.

Western blot analysis of extracts from HuvEc, using SIRPB1 Antibody.

Figure 1 The PERK pathway but not the ATF-6 or IRE-1 pathway was activated.

Figure 1: |The PERK pathway but not the ATF-6 or IRE-1 pathway was activated.

Product:	SIRPB1 Antibody
Catalog:	DF4507
Description:	Rabbit polyclonal antibody to SIRPB1
Application:	WB IF/ICC
Reactivity:	Human, Rat
Mol.Wt.:	48 KD; 43kD(Calculated).
Uniprot:	O00241
RRID:	AB_2836858

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Clonality:

Polyclonal

Specificity:

SIRPB1 Antibody detects endogenous levels of total SIRPB1.

RRID:

AB_2836858
Cite Format: Affinity Biosciences Cat# DF4507, RRID:AB_2836858.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD172 antigen like family member B; CD172b; CD172b antigen; DKFZp686A05192; Signal regulatory protein beta 1; SIRP BETA 1; SIRP beta 1 isoform 3;

Immunogens

Immunogen:

Uniprot:

O00241(SIRB1_HUMAN) >>Visit HPA database.

Gene(ID):

SIRPB1(10326)

Expression:

O00241 SIRB1_HUMAN:

Detected in monocytes and dendritic cells.

Sequence:

O00241 SIRB1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPVPASWPHLPSPFLLMTLLLGRLTGVAGEDELQVIQPEKSVSVAAGESATLRCAMTSLIPVGPIMWFRGAGAGRELIYNQKEGHFPRVTTVSELTKRNNLDFSISISNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAVRATPEHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPAGDSVSYSIHSTARVVLTRGDVHSQVICEIAHITLQGDPLRGTANLSEAIRVPPTLEVTQQPMRAENQANVTCQVSNFYPRGLQLTWLENGNVSRTETASTLIENKDGTYNWMSWLLVNTCAHRDDVVLTCQVEHDGQQAVSKSYALEISAHQKEHGSDITHEAALAPTAPLLVALLLGPKLLLVVGVSAIYICWKQKA

PTMs - O00241 As Substrate

O00241

Site	PTM Type	Source
K82	Ubiquitination	Uniprot
K97	Ubiquitination	Uniprot
Y119	Phosphorylation	Uniprot
K182	Ubiquitination	Uniprot
N244	N-Glycosylation	Uniprot

Research Backgrounds

O00241_SIRB1_HUMAN

Function:

Immunoglobulin-like cell surface receptor involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Participates also in the recruitment of tyrosine kinase SYK. Triggers activation of myeloid cells when associated with TYROBP.

PTMs:

N-glycosylated.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Tissue Specificity:

Detected in monocytes and dendritic cells.

Subunit Structure:

Homodimer; disulfide-linked. Interacts with TYROBP. This interaction results in the recruitment of SYK.

Research Fields

· Organismal Systems > Development > Osteoclast differentiation. (View pathway)

References

1). Suppression of CHOP Reduces Neuronal Apoptosis and Rescues Cognitive Impairment Induced by Intermittent Hypoxia by Inhibiting Bax and Bak Activation. Neural Plasticity, 2021 (PubMed: 34471408) [IF=3.1]

Application: WB Species: Mouse Sample: hippocampus tissue

Figure 1 The PERK pathway but not the ATF-6 or IRE-1 pathway was activated. (a) The expression levels of p-PERK, p-elf2α, and ATF-4 were increased in the hippocampus after 2 weeks of IH exposure (n = 4). The expression of p-IRE1 (b) and p-ATF-6 (c) was not altered (n = 4). ∗P < 0.05; ∗∗P < 0.01; ns: not significant.

Application: WB Species: mouse Sample: hippocampal

Figure 1: |The PERK pathway but not the ATF-6 or IRE-1 pathway was activated. (a) The expression levels of p-PERK, p-elf2α, and ATF-4 were increased in the hippocampus after 2 weeks of IH exposure (n = 4). The expression of p-IRE1 (b) and p-ATF-6 (c) was not altered(n = 4). ∗P < 0:05; ∗∗P < 0:01; ns: not significant.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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SIRPB1 Antibody - #DF4507