SH3GL2 Antibody - #DF4505

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Product: SH3GL2 Antibody
Catalog: DF4505
Description: Rabbit polyclonal antibody to SH3GL2
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 39 KD; 40kD(Calculated).
Uniprot: Q99962
RRID: AB_2836856

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
SH3GL2 Antibody detects endogenous levels of total SH3GL2.
RRID:
AB_2836856
Cite Format: Affinity Biosciences Cat# DF4505, RRID:AB_2836856.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bA335L15.1 (SH3 domain GRB2 like 2); CNSA2; EEN B1; EEN-B1; Endophilin 1; Endophilin A1; Endophilin A1 BAR domain; Endophilin-1; Endophilin-A1; FLJ20276; FLJ25015; OTTHUMP00000021084; SH3 domain containing GRB2 like protein 2; SH3 domain GRB2 like 2; SH3 domain protein 2A; SH3 domain-containing GRB2-like protein 2; SH3D2A; SH3G2_HUMAN; Sh3gl2; Sh3p4; SH3PA;

Immunogens

Immunogen:
Uniprot:

Q99962(SH3G2_HUMAN) >>Visit HPA database.

Gene(ID):
SH3GL2(6456)
Expression:
Q99962 SH3G2_HUMAN:

Brain, mostly in frontal cortex. Expressed at high level in fetal cerebellum.

Sequence:
MSVAGLKKQFHKATQKVSEKVGGAEGTKLDDDFKEMERKVDVTSRAVMEIMTKTIEYLQPNPASRAKLSMINTMSKIRGQEKGPGYPQAEALLAEAMLKFGRELGDDCNFGPALGEVGEAMRELSEVKDSLDIEVKQNFIDPLQNLHDKDLREIQHHLKKLEGRRLDFDYKKKRQGKIPDEELRQALEKFDESKEIAESSMFNLLEMDIEQVSQLSALVQAQLEYHKQAVQILQQVTVRLEERIRQASSQPRREYQPKPRMSLEFPTGDSTQPNGGLSHTGTPKPSGVQMDQPCCRALYDFEPENEGELGFKEGDIITLTNQIDENWYEGMLHGHSGFFPINYVEILVALPH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
73
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99962 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
T14 Phosphorylation O75116 (ROCK2)
S75 Phosphorylation Q5S007 (LRRK2)
K76 Acetylation
K149 Ubiquitination
K159 Ubiquitination
K177 Ubiquitination
R184 Methylation
S249 Phosphorylation

Research Backgrounds

Function:

Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature. Required for BDNF-dependent dendrite outgrowth. Cooperates with SH3GL2 to mediate BDNF-NTRK2 early endocytic trafficking and signaling from early endosomes.

Subcellular Location:

Cytoplasm. Membrane>Peripheral membrane protein. Early endosome. Cell junction>Synapse>Presynapse.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Brain, mostly in frontal cortex. Expressed at high level in fetal cerebellum.

Subunit Structure:

Monomer; in cytoplasm. Homodimer; when associated with membranes (By similarity). Interacts with OPHN1 (By similarity). Interacts with SYNJ1. Interacts with DNM1 (By similarity). Interacts with MAP4K3; the interaction appears to regulate MAP4K3-mediated JNK activation (By similarity). Interacts with PDCD6IP. Interacts with ATX2. Interacts with ADAM9 and ADAM15 cytoplasmic tails. Interacts with BIN2. Interacts with TMEM108 (By similarity). Interacts with ADGRB2.

Family&Domains:

An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes. The BAR domain dimer forms a rigid crescent shaped bundle of helices with the pair of second amphipathic helices protruding towards the membrane-binding surface.

Belongs to the endophilin family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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