SHANK2 Antibody - #DF4501

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Product: SHANK2 Antibody
Catalog: DF4501
Description: Rabbit polyclonal antibody to SHANK2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 135 KD; 159kD(Calculated).
Uniprot: Q9UPX8
RRID: AB_2836852

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
SHANK2 Antibody detects endogenous levels of total SHANK2.
RRID:
AB_2836852
Cite Format: Affinity Biosciences Cat# DF4501, RRID:AB_2836852.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AUTS17; Cortactin binding protein 1; Cortactin SH3 domain-binding protein; Cortactin-binding protein 1; CortBP1; CTTNBP1; GKAP/SAPAP interacting protein; GKAP/SAPAP-interacting protein; KIAA1022; Proline rich synapse associated protein 1; Proline-rich synapse-associated protein 1; ProSAP1; SH3 and multiple ankyrin repeat domains 2; SH3 and multiple ankyrin repeat domains protein 2; SHAN2_RAT; SHANK; Shank2; SPANK-3;

Immunogens

Immunogen:
Uniprot:

Q9UPX8(SHAN2_HUMAN) >>Visit HPA database.

Gene(ID):
SHANK2(22941)
Expression:
Q9UPX8 SHAN2_HUMAN:

Isoform 3 is present in epithelial colonic cells (at protein level).

Sequence:
MKSLLNAFTKKEVPFREAPAYSNRRRRPPNTLAAPRVLLRSNSDNNLNASAPDWAVCSTATSHRSLSPQLLQQMPSKPEGAAKTIGSYVPGPRSRSPSLNRLGGAGEDGKRPQPLWHVGSPFALGANKDSLSAFEYPGPKRKLYSAVPGRLFVAVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVQCKPRDSQAETRADRSKKLFRHYTVGSYDSFDTSSDCIIEEKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVNMIRQGGNHLVLKVVTVTRNLDPDDTARKKAPPPPKRAPTTALTLRSKSMTSELEELVDKASVRKKKDKPEEIVPASKPSRAAENMAVEPRVATIKQRPSSRCFPAGSDMNSVYERQGIAVMTPTVPGSPKAPFLGIPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTTYNCPKSPTPRVYGTIKPAFNQNSAAKVSPATRSDTVATMMREKGMYFRRELDRYSLDSEDLYSRNAGPQANFRNKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSMEIDPQAPEPPSQLRPDESLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRTRPSMFPEEGDFADEDSAEQLSSPMPSATPREPENHFVGGAEASAPGEAGRPLNSTSKAQGPESSPAVPSASSGTAGPGNYVHPLTGRLLDPSSPLALALSARDRAMKESQQGPKGEAPKADLNKPLYIDTKMRPSLDAGFPTVTRQNTRGPLRRQETENKYETDLGRDRKGDDKKNMLIDIMDTSQQKSAGLLMVHTVDATKLDNALQEEDEKAEVEMKPDSSPSEVPEGVSETEGALQISAAPEPTTVPGRTIVAVGSMEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRAASVPALSDLVKQKKSDTPQSPSLNSSQPTNSADSKKPASLSNCLPASFLPPPESFDAVADSGIEEVDSRSSSDHHLETTSTISTVSSISTLSSEGGENVDTCTVYADGQAFMVDKPPVPPKPKMKPIIHKSNALYQDALVEEDVDSFVIPPPAPPPPPGSAQPGMAKVLQPRTSKLWGDVTEIKSPILSGPKANVISELNSILQQMNREKLAKPGEGLDSPMGAKSASLAPRSPEIMSTISGTRSTTVTFTVRPGTSQPITLQSRPPDYESRTSGTRRAPSPVVSPTEMNKETLPAPLSAATASPSPALSDVFSLPSQPPSGDLFGLNPAGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Chicken
100
Rabbit
100
Bovine
91
Sheep
91
Xenopus
64
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UPX8 As Substrate

Site PTM Type Enzyme
S41 Phosphorylation
S43 Phosphorylation
S65 Phosphorylation
S67 Phosphorylation
S76 Phosphorylation
T84 Phosphorylation
S87 Phosphorylation
Y88 Phosphorylation
S94 Phosphorylation
S120 Phosphorylation
Y144 Phosphorylation
S231 Phosphorylation
S234 Phosphorylation
T339 Phosphorylation
T341 Phosphorylation
T349 Phosphorylation
T363 Phosphorylation
T367 Phosphorylation
S372 Phosphorylation
Y437 Phosphorylation
S452 Phosphorylation
S470 Phosphorylation
S478 Phosphorylation
S518 Phosphorylation
Y538 Phosphorylation
S549 Phosphorylation
S559 Phosphorylation
T561 Phosphorylation
T564 Phosphorylation
Y580 Phosphorylation
S581 Phosphorylation
S584 Phosphorylation
Y588 Phosphorylation
Y610 Phosphorylation
S611 Phosphorylation
Y622 Phosphorylation
S643 Phosphorylation
S649 Phosphorylation
T662 Phosphorylation
S664 Phosphorylation
S665 Phosphorylation
S720 Phosphorylation
S725 Phosphorylation
T726 Phosphorylation
S813 Phosphorylation
Y822 Phosphorylation
T827 Phosphorylation
S835 Phosphorylation
Y869 Phosphorylation
S877 Phosphorylation
T890 Phosphorylation
T899 Phosphorylation
Y903 Phosphorylation
S931 Phosphorylation
T939 Phosphorylation
T943 Phosphorylation
S1001 Phosphorylation
S1051 Phosphorylation
S1064 Phosphorylation
S1069 Phosphorylation
S1071 Phosphorylation
S1080 Phosphorylation
S1103 Phosphorylation
S1234 Phosphorylation
S1269 Phosphorylation
S1282 Phosphorylation
S1330 Phosphorylation
S1334 Phosphorylation
S1382 Phosphorylation
S1384 Phosphorylation
S1386 Phosphorylation
S1395 Phosphorylation

Research Backgrounds

Function:

Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.

Subcellular Location:

Apical cell membrane. Cytoplasm. Cell junction>Synapse. Cell junction>Synapse>Postsynaptic density. Cell projection>Growth cone. Cell projection>Dendritic spine.
Note: Colocalizes with cortactin in growth cones in differentiating hippocampal neurons. Colocalized with PDE4D to the apical membrane of colonic crypt cells (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 3 is present in epithelial colonic cells (at protein level).

Subunit Structure:

Is part of a complex with DLG4/PSD-95 and DLGAP1/GKAP. Interacts with CTTN/cortactin SH3 domain, DLGAP1/GKAP and alpha-latrotoxin receptor 1. Interacts with DNM2, DBNL, GRID2, BAIAP2, SLC9A3, PLCB3 and CFTR. Interacts (via proline-rich region) with PDE4D. Interacts with ABI1 (via SH3 domain).

Family&Domains:

The PDZ domain is required for interaction with GRID2, PLCB3, CFTR and SLC9A3.

Belongs to the SHANK family.

Research Fields

· Organismal Systems > Nervous system > Glutamatergic synapse.

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