Product: SERPINB4 Antibody
Catalog: DF4491
Description: Rabbit polyclonal antibody to SERPINB4
Application: WB IF/ICC
Reactivity: Human
Mol.Wt.: 45 KD; 45kD(Calculated).
Uniprot: P48594
RRID: AB_2836842

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
SERPINB4 Antibody detects endogenous levels of total SERPINB4.
RRID:
AB_2836842
Cite Format: Affinity Biosciences Cat# DF4491, RRID:AB_2836842.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Leupin; Peptidase inhibitor 11; PI 11; PI-11; PI11; Protease inhibitor (leucine serpin); SCCA 2; SCCA-2; SCCA1; SCCA2; SCCA2/SCCA1 fusion protein; Serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 4; Serpin B4; Serpin peptidase inhibitor, clade B (ovalbumin), member 4; SERPINB 4; SERPINB4; SPB4_HUMAN; Squamous cell carcinoma antigen 1; Squamous cell carcinoma antigen 2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P48594 SPB4_HUMAN:

Squamous cells.

Sequence:
MNSLSEANTKFMFDLFQQFRKSKENNIFYSPISITSALGMVLLGAKDNTAQQISKVLHFDQVTENTTEKAATYHVDRSGNVHHQFQKLLTEFNKSTDAYELKIANKLFGEKTYQFLQEYLDAIKKFYQTSVESTDFANAPEESRKKINSWVESQTNEKIKNLFPDGTIGNDTTLVLVNAIYFKGQWENKFKKENTKEEKFWPNKNTYKSVQMMRQYNSFNFALLEDVQAKVLEIPYKGKDLSMIVLLPNEIDGLQKLEEKLTAEKLMEWTSLQNMRETCVDLHLPRFKMEESYDLKDTLRTMGMVNIFNGDADLSGMTWSHGLSVSKVLHKAFVEVTEEGVEAAAATAVVVVELSSPSTNEEFCCNHPFLFFIRQNKTNSILFYGRFSSP

PTMs - P48594 As Substrate

Site PTM Type Enzyme
Y73 Phosphorylation
Y127 Phosphorylation
S130 Phosphorylation
S149 Phosphorylation
S153 Phosphorylation
Y181 Phosphorylation
T347 Phosphorylation
S355 Phosphorylation
S356 Phosphorylation
T359 Phosphorylation
Y384 Phosphorylation

Research Backgrounds

Function:

May act as a protease inhibitor to modulate the host immune response against tumor cells.

Subcellular Location:

Cytoplasm.
Note: Seems to also be secreted in plasma by cancerous cells but at a low level.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Squamous cells.

Family&Domains:

Belongs to the serpin family. Ov-serpin subfamily.

Research Fields

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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