PPP2R5D Antibody - #DF4483

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Product: PPP2R5D Antibody
Catalog: DF4483
Description: Rabbit polyclonal antibody to PPP2R5D
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 70 KD; 70kD(Calculated).
Uniprot: Q14738
RRID: AB_2836834

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
PPP2R5D Antibody detects endogenous levels of total PPP2R5D.
RRID:
AB_2836834
Cite Format: Affinity Biosciences Cat# DF4483, RRID:AB_2836834.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2A5D_HUMAN; B'delta; B56D; Delta isoform of regulatory subunit B56, protein phosphatase 2A; MGC2134; MGC8949; OTTHUMP00000039821; PP2A B subunit B' delta isoform; PP2A B subunit B56 delta isoform; PP2A B subunit isoform B''-delta; PP2A B subunit isoform B56-delta; PP2A B subunit isoform PR61-delta; PP2A B subunit isoform R5-delta; PP2A B subunit PR61 delta isoform; PP2A B subunit R5 delta isoform; PPP2R5D; Protein phosphatase 2 regulatory subunit B (B56) delta isoform; Protein phosphatase 2 regulatory subunit B delta isoform; Protein phosphatase 2 regulatory subunit B' delta; Serine threonine protein phosphatase 2A 56 kDa regulatory subunit delta isoform; Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform; TEG-271; Tex271;

Immunogens

Immunogen:
Uniprot:

Q14738(2A5D_HUMAN) >>Visit HPA database.

Gene(ID):
PPP2R5D(5528)
Expression:
Q14738 2A5D_HUMAN:

Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain.

Sequence:
MPYKLKKEKEPPKVAKCTAKPSSSGKDGGGENTEEAQPQPQPQPQPQAQSQPPSSNKRPSNSTPPPTQLSKIKYSGGPQIVKKERRQSSSRFNLSKNRELQKLPALKDSPTQEREELFIQKLRQCCVLFDFVSDPLSDLKFKEVKRAGLNEMVEYITHSRDVVTEAIYPEAVTMFSVNLFRTLPPSSNPTGAEFDPEEDEPTLEAAWPHLQLVYEFFLRFLESPDFQPNIAKKYIDQKFVLALLDLFDSEDPRERDFLKTILHRIYGKFLGLRAYIRRQINHIFYRFIYETEHHNGIAELLEILGSIINGFALPLKEEHKMFLIRVLLPLHKVKSLSVYHPQLAYCVVQFLEKESSLTEPVIVGLLKFWPKTHSPKEVMFLNELEEILDVIEPSEFSKVMEPLFRQLAKCVSSPHFQVAERALYYWNNEYIMSLISDNAARVLPIMFPALYRNSKSHWNKTIHGLIYNALKLFMEMNQKLFDDCTQQYKAEKQKGRFRMKEREEMWQKIEELARLNPQYPMFRAPPPLPPVYSMETETPTAEDIQLLKRTVETEAVQMLKDIKKEKVLLRRKSELPQDVYTIKALEAHKRAEEFLTASQEAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
78
Pig
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14738 As Substrate

Site PTM Type Enzyme
T18 Phosphorylation
S54 Phosphorylation
S60 Phosphorylation P17612 (PRKACA) , O14757 (CHEK1)
S62 Phosphorylation
T63 Phosphorylation
T67 Phosphorylation
K71 Ubiquitination
K73 Ubiquitination
Y74 Phosphorylation
S75 Phosphorylation P17612 (PRKACA)
K82 Ubiquitination
S88 Phosphorylation
S89 Phosphorylation
S90 Phosphorylation
S95 Phosphorylation
K96 Ubiquitination
K102 Ubiquitination
K107 Ubiquitination
S109 Phosphorylation
K121 Ubiquitination
K140 Ubiquitination
K232 Ubiquitination
K268 Ubiquitination
K332 Ubiquitination
K334 Ubiquitination
K367 Ubiquitination
K371 Ubiquitination
K409 Ubiquitination
Y451 Phosphorylation
T461 Phosphorylation
K479 Ubiquitination
Y488 Phosphorylation
K489 Ubiquitination
K500 Ubiquitination
K508 Ubiquitination
Y519 Phosphorylation
R523 Methylation
Y532 Phosphorylation
S533 Phosphorylation
T536 Phosphorylation
T538 Phosphorylation
K548 Ubiquitination
T553 Phosphorylation
K560 Ubiquitination
K572 Ubiquitination
S573 Phosphorylation P17612 (PRKACA)
Y580 Phosphorylation
T581 Phosphorylation
K583 Ubiquitination
K589 Ubiquitination
S598 Phosphorylation

Research Backgrounds

Function:

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Nuclear in interphase, nuclear during mitosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain.

Subunit Structure:

PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with SGO1. Interacts with ADCY8.

Family&Domains:

Belongs to the phosphatase 2A regulatory subunit B56 family.

Research Fields

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Nervous system > Dopaminergic synapse.

Restrictive clause

 

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