NEK7 Antibody - #DF4467

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Product: NEK7 Antibody
Catalog: DF4467
Description: Rabbit polyclonal antibody to NEK7
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 35 KD; 35kD(Calculated).
Uniprot: Q8TDX7
RRID: AB_2836822

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
NEK7 Antibody detects endogenous levels of total NEK7.
RRID:
AB_2836822
Cite Format: Affinity Biosciences Cat# DF4467, RRID:AB_2836822.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EC 2.7.11.1; NEK7; NEK7_HUMAN; Never in mitosis A-related kinase 7; Never in mitosis gene a-related kinase 7; NIMA (never in mitosis gene a) related kinase 7; NIMA (never in mitosis gene a)-related expressed kinase 7; NimA related protein kinase 7; NIMA-related kinase 7; NimA-related protein kinase 7; Serine/threonine-protein kinase Nek7;

Immunogens

Immunogen:
Uniprot:

Q8TDX7(NEK7_HUMAN) >>Visit HPA database.

Gene(ID):
NEK7(140609)
Expression:
Q8TDX7 NEK7_HUMAN:

Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.

Sequence:
MDEQSQGMQGPPVPQFQPQKALRPDMGYNTLANFRIEKKIGRGQFSEVYRAACLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKHFKKQKRLIPERTVWKYFVQLCSALEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRQLVNMCINPDPEKRPDVTYVYDVAKRMHACTASS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
78
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8TDX7 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S5 Phosphorylation
K20 Ubiquitination
T30 Phosphorylation
K63 Ubiquitination
K64 Ubiquitination
K74 Ubiquitination
K81 Ubiquitination
K87 Ubiquitination
Y97 Phosphorylation
S147 Phosphorylation
K163 Ubiquitination
T170 Phosphorylation
T172 Phosphorylation
K176 Ubiquitination
S187 Phosphorylation
S188 Phosphorylation
K189 Ubiquitination
T190 Phosphorylation
T191 Phosphorylation
S195 Phosphorylation Q8TD19 (NEK9)
T199 Phosphorylation
Y201 Phosphorylation
Y202 Phosphorylation
S204 Phosphorylation
Y244 Phosphorylation
S245 Phosphorylation
K248 Ubiquitination
K249 Ubiquitination
K281 Ubiquitination
T286 Phosphorylation
Y287 Phosphorylation
Y289 Phosphorylation
K293 Ubiquitination

PTMs - Q8TDX7 As Enzyme

Substrate Site Source
P23443 (RPS6KB1) T412 Uniprot
P54274 (TERF1) S114 Uniprot

Research Backgrounds

Function:

Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1.

PTMs:

Phosphorylation at Ser-195 required for its activation.

Subcellular Location:

Nucleus. Cytoplasm. Cytoplasm>Cytoskeleton>Spindle pole. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Present at centrosome throughout the cell cycle (PubMed:17586473). Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody (PubMed:17586473). Interaction with ANKS3 prevents its translocation to the nucleus (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.

Subunit Structure:

Monomer. Interacts with NEK9. Interacts with ANKS3; this interaction alters the subcellular distribution of NEK7 by preventing its nuclear translocation.

Family&Domains:

Displays an autoinhibited conformation: Tyr-97 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9.

The NTE (N-terminal extension) motif is a structural component of the catalytic domain and thus contributes to activity.

Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.

Research Fields

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

References

1). Quercetin-induced pyroptosis in colon cancer through NEK7-mediated NLRP3 inflammasome-GSDMD signaling pathway activation. American journal of cancer research, 2024 (PubMed: 38590424) [IF=5.3]

Application: WB    Species: Human    Sample: colon cancer tissues

Figure 7 Downregulated expression of NEK7 in human colon cancer tissues and correlated with NLRP3 expression. A. Gene correlation between NLRP3 and NEK7 in colon cancer investigated using the TIMER2.0 database based on Spearman’s correlation analysis. Rho represents the correlation index. B. Influence of NEK7 knockdown on NLRP3 protein expression determined using western blotting (n=3, where “n” represents the number of independent repeated experiments). Values are presented as mean ± SD. *P
2). Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy. Oxidative Medicine and Cellular Longevity, 2022 (PubMed: 35498125)

Application: WB    Species: Rat    Sample: diabetic myocardium

"Figure 4 SG attenuates the activation of NLRP3 in diabetic myocardium. (a) Protein levels of NLRP3-related molecules shown by western blot. β-Tubulin was an internal reference control. n = 3 in each group. (b) Relative mRNA levels of Nlrp3-relaterd genes shown by real-time RT-PCR. Tubb3 served as a reference gene. n = 3 in each group. (c) Ratio of caspase-1 p12/procaspase-1. n = 3 in each group. (d) Caspase-1 activity in heart tissues. n = 6 in each group. Immunohistochemical staining of (e) NLRP3, (f) ASC, (g) GSDMD, (h) IL-1β, (i) IL-18, and (j) NEK7. Scale bar, 50 μm. (k) Double-labeling immunofluorescence of NLRP3 and CD68. Scale bar, 50 μm. Data are presented as mean ± SD. ∗∗P < 0.01 CON vs. SHAM; ##P < 0.01 SG vs. SHAM. n = 3 in each group."

Application: IHC    Species: Rat    Sample: diabetic myocardium

"Figure 4 SG attenuates the activation of NLRP3 in diabetic myocardium. (a) Protein levels of NLRP3-related molecules shown by western blot. β-Tubulin was an internal reference control. n = 3 in each group. (b) Relative mRNA levels of Nlrp3-relaterd genes shown by real-time RT-PCR. Tubb3 served as a reference gene. n = 3 in each group. (c) Ratio of caspase-1 p12/procaspase-1. n = 3 in each group. (d) Caspase-1 activity in heart tissues. n = 6 in each group. Immunohistochemical staining of (e) NLRP3, (f) ASC, (g) GSDMD, (h) IL-1β, (i) IL-18, and (j) NEK7. Scale bar, 50 μm. (k) Double-labeling immunofluorescence of NLRP3 and CD68. Scale bar, 50 μm. Data are presented as mean ± SD. ∗∗P < 0.01 CON vs. SHAM; ##P < 0.01 SG vs. SHAM. n = 3 in each group."

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