MST3 Antibody - #DF4463

(1)   (2)  
Product: MST3 Antibody
Catalog: DF4463
Description: Rabbit polyclonal antibody to MST3
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Rabbit, Dog
Mol.Wt.: 50 KD; 49kD(Calculated).
Uniprot: Q9Y6E0
RRID: AB_2836818

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
MST3 Antibody detects endogenous levels of total MST3.
RRID:
AB_2836818
Cite Format: Affinity Biosciences Cat# DF4463, RRID:AB_2836818.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

epididymis secretory protein Li 95; HEL-S-95; Mammalian STE20 like protein kinase 3; Mammalian STE20-like protein kinase 3; Mammalian STE20-like protein kinase 3 C-terminal; Mammalian STE20-like protein kinase 3 N-terminal; Mammalian sterile 20-like 3; MST-3; MST3/C; MST3/N; MST3B; serine threonine kinase 24; Serine/threonine kinase 24 (Ste20, yeast homolog); Serine/threonine-protein kinase 24 12 kDa subunit; Serine/threonine-protein kinase 24; STE20 homolog yeast; STE20 like kinase MST3; STE20-like kinase MST3; Stk24; STK24_HUMAN; STK3;

Immunogens

Immunogen:
Uniprot:

Q9Y6E0(STK24_HUMAN) >>Visit HPA database.

Gene(ID):
STK24(8428)
Expression:
Q9Y6E0 STK24_HUMAN:

Isoform A is ubiquitous. Isoform B is expressed in brain with high expression in hippocampus and cerebral cortex.

Sequence:
MDSRAQLWGLALNKRRATLPHPGGSTNLKADPEELFTKLEKIGKGSFGEVFKGIDNRTQKVVAIKIIDLEEAEDEIEDIQQEITVLSQCDSPYVTKYYGSYLKDTKLWIIMEYLGGGSALDLLEPGPLDETQIATILREILKGLDYLHSEKKIHRDIKAANVLLSEHGEVKLADFGVAGQLTDTQIKRNTFVGTPFWMAPEVIKQSAYDSKADIWSLGITAIELARGEPPHSELHPMKVLFLIPKNNPPTLEGNYSKPLKEFVEACLNKEPSFRPTAKELLKHKFILRNAKKTSYLTELIDRYKRWKAEQSHDDSSSEDSDAETDGQASGGSDSGDWIFTIREKDPKNLENGALQPSDLDRNKMKDIPKRPFSQCLSTIISPLFAELKEKSQACGGNLGSIEELRGAIYLAEEACPGISDTMVAQLVQRLQRYSLSGGGTSSH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
88
Dog
88
Pig
0
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6E0 As Substrate

Site PTM Type Enzyme
T18 Phosphorylation P17612 (PRKACA)
K38 Ubiquitination
K44 Ubiquitination
S46 Phosphorylation
K52 Ubiquitination
Y93 Phosphorylation
K142 Ubiquitination
K151 Ubiquitination
T182 Phosphorylation
T184 Phosphorylation
K187 Ubiquitination
T190 Phosphorylation Q9Y6E0 (STK24)
T194 Phosphorylation
K238 Ubiquitination
K245 Ubiquitination
K257 Ubiquitination
K278 Ubiquitination
K292 Ubiquitination
S294 Phosphorylation
Y303 Phosphorylation
S315 Phosphorylation
S316 Phosphorylation
S317 Phosphorylation
S320 Phosphorylation
K347 Ubiquitination
S381 Phosphorylation
K390 Ubiquitination
S400 Phosphorylation
Y433 Phosphorylation
S434 Phosphorylation

PTMs - Q9Y6E0 As Enzyme

Substrate Site Source
Q15208 (STK38) T444 Uniprot
Q8TAE6 (PPP1R14C) T73 Uniprot
Q9Y2H1 (STK38L) T442 Uniprot
Q9Y6E0 (STK24) T190 Uniprot

Research Backgrounds

Function:

Serine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. In association with STK26 negatively regulates Golgi reorientation in polarized cell migration upon RHO activation. Regulates also cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. May act as a key regulator of axon regeneration in the optic nerve and radial nerve.

PTMs:

Proteolytically processed by caspases during apoptosis. Proteolytic cleavage results in kinase activation, nuclear translocation of the truncated form (MST3/N) and the induction of apoptosis.

Isoform B is activated by phosphorylation by PKA. Oxidative stress induces phosphorylation. Activated by autophosphorylation at Thr-190 and phosphorylation at this site is essential for its function. Manganese, magnesium and cobalt-dependent autophosphorylation is mainly on threonine residues while zinc-dependent autophosphorylation is on both serine and threonine residues.

Subcellular Location:

Cytoplasm. Nucleus. Membrane.
Note: The truncated form (MST3/N) translocates to the nucleus. Colocalizes with STK38L in the membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform A is ubiquitous. Isoform B is expressed in brain with high expression in hippocampus and cerebral cortex.

Subunit Structure:

Monomer. Interacts with CTTNBP2NL. Interacts with RIPOR1 (via C-terminus); this interaction occurs in a PDCD10-dependent and Rho-independent manner. Interacts with PDCD10; this interaction is required for the association of STK24 with RIPOR1.

Family&Domains:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

References

1). STK24 modulates excitatory synaptic transmission in epileptic hippocampal neurons. CNS Neuroscience & Therapeutics, 2020 (PubMed: 32436359) [IF=5.5]

Application: WB    Species: mouse    Sample: hippocampus

FIGURE 5| STK24 is a potential binding partner of the NMDAR complex in the hippocampus of PTZ-kindled mice. A, STK24 interacts with the NMDAR complex, but not with the AMPAR complex. Hippocampal lysates from PTZkindled mice were immunoprecipitated with STK24 antibody and incubated with corresponding antibodies (IB: immunoblotting, IP: immunoprecipitation). B–F, Lysates from hippocampus of PTZkindled mice were immunoprecipitated with antibodies against NR1(B), NR2A(C), NR2B(D), GluR1(E), GluR2(F), and incubated with corresponding antibodies

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.