SPINK5 Antibody - #DF4462

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Product: SPINK5 Antibody
Catalog: DF4462
Description: Rabbit polyclonal antibody to SPINK5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 121 KD; 121kD(Calculated).
Uniprot: Q9NQ38
RRID: AB_2836817

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(82%), Sheep(100%), Rabbit(100%), Dog(91%)
Clonality:
Polyclonal
Specificity:
SPINK5 Antibody detects endogenous levels of total SPINK5.
RRID:
AB_2836817
Cite Format: Affinity Biosciences Cat# DF4462, RRID:AB_2836817.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Hemofiltrate peptide HF7665; ISK5_HUMAN; LEKTI; Lympho-epithelial Kazal-type-related inhibitor; Lymphoepithelial Kazal type related inhibitor; NETS; NS; Serine peptidase inhibitor Kazal type 5; SPINK5; VAKTI;

Immunogens

Immunogen:
Uniprot:

Q9NQ38(ISK5_HUMAN) >>Visit HPA database.

Gene(ID):
SPINK5(11005)
Expression:
Q9NQ38 ISK5_HUMAN:

Highly expressed in the thymus and stratum corneum. Also found in the oral mucosa, parathyroid gland, Bartholin's glands, tonsils, and vaginal epithelium. Very low levels are detected in lung, kidney, and prostate.

Sequence:
MKIATVSVLLPLALCLIQDAASKNEDQEMCHEFQAFMKNGKLFCPQDKKFFQSLDGIMFINKCATCKMILEKEAKSQKRARHLARAPKATAPTELNCDDFKKGERDGDFICPDYYEAVCGTDGKTYDNRCALCAENAKTGSQIGVKSEGECKSSNPEQDVCSAFRPFVRDGRLGCTRENDPVLGPDGKTHGNKCAMCAELFLKEAENAKREGETRIRRNAEKDFCKEYEKQVRNGRLFCTRESDPVRGPDGRMHGNKCALCAEIFKQRFSEENSKTDQNLGKAEEKTKVKREIVKLCSQYQNQAKNGILFCTRENDPIRGPDGKMHGNLCSMCQAYFQAENEEKKKAEARARNKRESGKATSYAELCSEYRKLVRNGKLACTRENDPIQGPDGKVHGNTCSMCEVFFQAEEEEKKKKEGKSRNKRQSKSTASFEELCSEYRKSRKNGRLFCTRENDPIQGPDGKMHGNTCSMCEAFFQQEERARAKAKREAAKEICSEFRDQVRNGTLICTREHNPVRGPDGKMHGNKCAMCASVFKLEEEEKKNDKEEKGKVEAEKVKREAVQELCSEYRHYVRNGRLPCTRENDPIEGLDGKIHGNTCSMCEAFFQQEAKEKERAEPRAKVKREAEKETCDEFRRLLQNGKLFCTRENDPVRGPDGKTHGNKCAMCKAVFQKENEERKRKEEEDQRNAAGHGSSGGGGGNTQDECAEYREQMKNGRLSCTRESDPVRDADGKSYNNQCTMCKAKLEREAERKNEYSRSRSNGTGSESGKDTCDEFRSQMKNGKLICTRESDPVRGPDGKTHGNKCTMCKEKLEREAAEKKKKEDEDRSNTGERSNTGERSNDKEDLCREFRSMQRNGKLICTRENNPVRGPYGKMHINKCAMCQSIFDREANERKKKDEEKSSSKPSNNAKDECSEFRNYIRNNELICPRENDPVHGADGKFYTNKCYMCRAVFLTEALERAKLQEKPSHVRASQEEDSPDSFSSLDSEMCKDYRVLPRIGYLCPKDLKPVCGDDGQTYNNPCMLCHENLIRQTNTHIRSTGKCEESSTPGTTAASMPPSDE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Rabbit
100
Dog
91
Horse
82
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NQ38 As Substrate

Site PTM Type Enzyme
S147 Phosphorylation
T189 Phosphorylation
Y440 Phosphorylation
S725 Phosphorylation
T773 Phosphorylation
T838 Phosphorylation
T958 Phosphorylation

Research Backgrounds

Function:

Serine protease inhibitor, probably important for the anti-inflammatory and/or antimicrobial protection of mucous epithelia. Contribute to the integrity and protective barrier function of the skin by regulating the activity of defense-activating and desquamation-involved proteases. Inhibits KLK5, it's major target, in a pH-dependent manner. Inhibits KLK7, KLK14 CASP14, and trypsin.

PTMs:

Proteolytically processed by furin in individual domains (D1, D5, D6, D8 through D11, and D9 through D15) exhibiting various inhibitory potentials for multiple proteases.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in the thymus and stratum corneum. Also found in the oral mucosa, parathyroid gland, Bartholin's glands, tonsils, and vaginal epithelium. Very low levels are detected in lung, kidney, and prostate.

Family&Domains:

Contains at least one active inhibitory domain for trypsin (domain 6).

References

1). Dissecting the single-cell transcriptome network underlying esophagus non-malignant tissues and esophageal squamous cell carcinoma. eBioMedicine, 2021 (PubMed: 34192657) [IF=9.7]

Application: IF/ICC    Species: Rabbit    Sample: epithelial cells

Fig. 3. The single-cell transcriptomes of epithelial cells in non-malignant and malignant esophagus.(a) The TSNE plot and overview of epithelial cells by the cell type and origin of the cells. (b) Canonical cell markers were used to label epithelial subtypes as represented in the TSNE plot. (c) The TSNE plot of epithelial cells by the CNV’s levels of the cells. (d) Heatmap showing the expression of marker genes in malignant and non-malignant epithelial cells. (e) Violin plots showing the marker genes for each epithelial cell type. (f) Immunofluorescence staining of tumor (n = 8) (up) / normal (n = 8) (down) tissue-specific genes in ESCC and non-malignant tissues. Comparison of non-malignant and malig- nant esophagus was performed by Wilcoxon test. (g) Pseudotime analysis for malignant and non-malignant epithelial cells. (h) Differences in pathway activities scored per cell by GSVA between malignant and non-malignant epithelial cells. The scores of pathways are normalized.

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