SEPT6 Antibody - #DF4459

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Product: SEPT6 Antibody
Catalog: DF4459
Description: Rabbit polyclonal antibody to SEPT6
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 50 KD; 50kD(Calculated).
Uniprot: Q14141
RRID: AB_2836814

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Zebrafish(88%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(92%), Dog(92%)
Clonality:
Polyclonal
Specificity:
SEPT6 Antibody detects endogenous levels of total SEPT6.
RRID:
AB_2836814
Cite Format: Affinity Biosciences Cat# DF4459, RRID:AB_2836814.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

KIAA0128; Nkrf; RCG53214, isoform CRA_d; RP5-876A24.2; SEP2; SEPT2; SEPT6; SEPT6/MLL FUSION GENE; SEPT6_HUMAN; Septin 2; Septin 6; Septin-6;

Immunogens

Immunogen:
Uniprot:

Q14141(SEPT6_HUMAN) >>Visit HPA database.

Gene(ID):
SEPT6(23157)
Expression:
Q14141 SEPT6_HUMAN:

Widely expressed.

Sequence:
MAATDIARQVGEGCRTVPLAGHVGFDSLPDQLVNKSVSQGFCFNILCVGETGLGKSTLMDTLFNTKFEGEPATHTQPGVQLQSNTYDLQESNVRLKLTIVSTVGFGDQINKEDSYKPIVEFIDAQFEAYLQEELKIRRVLHTYHDSRIHVCLYFIAPTGHSLKSLDLVTMKKLDSKVNIIPIIAKADAISKSELTKFKIKITSELVSNGVQIYQFPTDDESVAEINGTMNAHLPFAVIGSTEELKIGNKMMRARQYPWGTVQVENEAHCDFVKLREMLIRVNMEDLREQTHTRHYELYRRCKLEEMGFKDTDPDSKPFSLQETYEAKRNEFLGELQKKEEEMRQMFVQRVKEKEAELKEAEKELHEKFDRLKKLHQDEKKKLEDKKKSLDDEVNAFKQRKTAAELLQSQGSQAGGSQTLKRDKEKKNNPWLCTE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
92
Bovine
92
Sheep
92
Dog
92
Rabbit
92
Zebrafish
88
Xenopus
77
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14141 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S27 Phosphorylation
T65 Phosphorylation
K66 Ubiquitination
S146 Phosphorylation
K171 Ubiquitination
K176 Ubiquitination
K185 Ubiquitination
K191 Ubiquitination
S192 Phosphorylation
K196 Acetylation
K196 Ubiquitination
K249 Ubiquitination
K273 Ubiquitination
Y295 Phosphorylation
Y298 Phosphorylation
K302 Ubiquitination
K309 Ubiquitination
T311 Phosphorylation
S315 Phosphorylation
K316 Ubiquitination
S319 Phosphorylation
Y324 Phosphorylation
K327 Ubiquitination
K337 Ubiquitination
K338 Ubiquitination
K351 Ubiquitination
K358 Ubiquitination
K362 Acetylation
K362 Ubiquitination
K367 Acetylation
K367 Ubiquitination
K379 Acetylation
K380 Acetylation
K387 Ubiquitination
S388 Phosphorylation
K397 Acetylation
K397 Ubiquitination
K400 Ubiquitination
S408 Phosphorylation
S411 Phosphorylation
S416 Phosphorylation
T418 Phosphorylation
K420 Ubiquitination
K426 Ubiquitination
C432 S-Nitrosylation

Research Backgrounds

Function:

Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Involved in cytokinesis. May play a role in HCV RNA replication. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton>Spindle. Chromosome>Centromere>Kinetochore. Cleavage furrow. Midbody. Cell projection>Cilium>Flagellum.
Note: In metaphase cells, localized within the microtubule spindle. At the metaphase plate, in close apposition to the kinetochores of the congressed chromosomes. In cells undergoing cytokinesis, localized to the midbody, the ingressing cleavage furrow, and the central spindle. Found in the sperm annulus (PubMed:25588830).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Septins polymerize into heterooligomeric protein complexes that form filaments, and associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation. Filaments are assembled from asymmetrical heterotrimers, composed of SEPTIN2, SEPTIN6 and SEPTIN7 that associate head-to-head to form a hexameric unit. Within the trimer, directly interacts with SEPTIN2 and SEPTIN7. Also interacts with SEPTIN9 and SEPTIN12. Interaction with SEPTIN12 alters filament structure. Component of a septin core octomeric complex consisting of SEPTIN12, SEPTIN7, SEPTIN6 and SEPTIN2 or SEPTIN4 in the order 12-7-6-2-2-6-7-12 or 12-7-6-4-4-6-7-12 and located in the sperm annulus. Interacts with SOCS7. Interacts with HNRNPA1.

(Microbial infection) Interacts with HCV NS5B.

Family&Domains:

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.

Research Fields

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

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