Product: ATP2A1 Antibody
Catalog: DF4449
Description: Rabbit polyclonal antibody to ATP2A1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 100 KD; 110kD(Calculated).
Uniprot: O14983
RRID: AB_2836804

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(82%), Sheep(100%), Rabbit(100%), Dog(91%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
ATP2A1 Antibody detects endogenous levels of total ATP2A1.
RRID:
AB_2836804
Cite Format: Affinity Biosciences Cat# DF4449, RRID:AB_2836804.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

fast twitch skeletal muscle isoform; AT2A1_HUMAN; ATP2A; ATP2A1; ATPase Ca++ transporting cardiac muscle fast twitch 1; ATPase Ca++ transporting fast twitch 1; ATPase, Ca(2+)-transporting fast twitch 1; Calcium pump 1; Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform; Calcium-transporting ATPase sarcoplasmic reticulum type; EC 3.6.3.8; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; Fast skeletal muscle SR calcium ATPase; OTTHUMP00000162561; OTTHUMP00000162562; Sarcoendoplasmic reticulum calcium ATPase; Sarcoplasmic reticulum Ca(2+)-ATPase 1; Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA 1; SERCA1; SERCA1 truncated isoform, included; SR Ca(2+) ATPase 1; SR Ca(2+)-ATPase 1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O14983 AT2A1_HUMAN:

Skeletal muscle, fast twitch muscle (type II) fibers.

Sequence:
MEAAHAKTTEECLAYFGVSETTGLTPDQVKRNLEKYGLNELPAEEGKTLWELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILIANAIVGVWQERNAENAIEALKEYEPEMGKVYRADRKSVQRIKARDIVPGDIVEVAVGDKVPADIRILAIKSTTLRVDQSILTGESVSVIKHTEPVPDPRAVNQDKKNMLFSGTNIAAGKALGIVATTGVGTEIGKIRDQMAATEQDKTPLQQKLDEFGEQLSKVISLICVAVWLINIGHFNDPVHGGSWFRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCKMFIIDKVDGDICLLNEFSITGSTYAPEGEVLKNDKPVRPGQYDGLVELATICALCNDSSLDFNEAKGVYEKVGEATETALTTLVEKMNVFNTDVRSLSKVERANACNSVIRQLMKKEFTLEFSRDRKSMSVYCSPAKSSRAAVGNKMFVKGAPEGVIDRCNYVRVGTTRVPLTGPVKEKIMAVIKEWGTGRDTLRCLALATRDTPPKREEMVLDDSARFLEYETDLTFVGVVGMLDPPRKEVTGSIQLCRDAGIRVIMITGDNKGTAIAICRRIGIFGENEEVADRAYTGREFDDLPLAEQREACRRACCFARVEPSHKSKIVEYLQSYDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMDRPPRSPKEPLISGWLFFRYMAIGGYVGAATVGAAAWWFLYAEDGPHVNYSQLTHFMQCTEDNTHFEGIDCEVFEAPEPMTMALSVLVTIEMCNALNSLSENQSLLRMPPWVNIWLLGSICLSMSLHFLILYVDPLPMIFKLRALDLTQWLMVLKISLPVIGLDEILKFVARNYLEDPEDERRK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Rabbit
100
Dog
91
Xenopus
91
Horse
82
Chicken
73
Zebrafish
55
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14983 As Substrate

Site PTM Type Enzyme
S19 Phosphorylation
K120 Ubiquitination
K128 Ubiquitination
S170 Phosphorylation
S184 Phosphorylation
S186 Phosphorylation
K205 Ubiquitination
K218 Acetylation
T230 Phosphorylation
K252 Ubiquitination
S261 Phosphorylation
Y294 Phosphorylation
K328 Ubiquitination
K329 Ubiquitination
S335 Phosphorylation
S338 Phosphorylation
C344 S-Nitrosylation
T345 Phosphorylation
S346 Phosphorylation
C349 S-Nitrosylation
S473 Phosphorylation
K481 Acetylation
K481 Ubiquitination
T484 Phosphorylation
S488 Phosphorylation
K492 Acetylation
Y497 Phosphorylation
K515 Acetylation
K515 Ubiquitination
Y527 Phosphorylation
S581 Phosphorylation
T608 Phosphorylation
T625 Phosphorylation
K684 Ubiquitination
K713 Ubiquitination
S722 Phosphorylation
T724 Phosphorylation
S731 Phosphorylation
S741 Phosphorylation
T742 Phosphorylation
K758 Acetylation
K758 Ubiquitination
S830 Phosphorylation

Research Backgrounds

Function:

Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen (By similarity). Contributes to calcium sequestration involved in muscular excitation/contraction.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Sarcoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Skeletal muscle, fast twitch muscle (type II) fibers.

Subunit Structure:

Interacts with sarcolipin (SLN) (By similarity). Interacts with phospholamban (PLN) (By similarity). Interacts with myoregulin (MRLN). Interacts with DWORF (By similarity).

Family&Domains:

Ca(2+) and ATP binding cause major rearrangements of the cytoplasmic and transmembrane domains. According to the E1-E2 model, Ca(2+) binding to the cytosolic domain of the pump in the high-affinity E1 conformation is followed by the ATP-dependent phosphorylation of the active site Asp, giving rise to E1P. A conformational change of the phosphoenzyme gives rise to the low-affinity E2P state that exposes the Ca(2+) ions to the lumenal side and promotes Ca(2+) release. Dephosphorylation of the active site Asp mediates the subsequent return to the E1 conformation.

PLN and SLN both have a single transmembrane helix; both occupy a similar binding site on ATP2A1 that is situated between the ATP2A1 transmembrane helices.

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Organismal Systems > Digestive system > Pancreatic secretion.

References

1). Rescuing SERCA2 pump deficiency improves bone mechano-responsiveness in type 2 diabetes by shaping osteocyte calcium dynamics. Nature communications, 2024 (PubMed: 38291059) [IF=16.6]

Application: WB    Species: Mouse    Sample:

Fig. 4 T2D-induced specific reduction of SERCA2 expression mediates an attenuation of osteocyte mechano-responsiveness. a Real-time intracellular Ca2+ signaling of osteocytes in situ in mouse tibiae treated with DMSO (vehicle control) and two antagonists of SERCA, i.e., thapsigargin (1 μM) and cyclopiazonic acid (CPA; 5 μM), in response to subsequent cyclic mechanical loading. b Intracellular Ca2+ signaling in MLO-Y4 osteocytic cells in vitro treated with DMSO, thapsigargin, and CPA in response to subsequent steady FSS stimulation at 2 Pa. c qRT-PCR analyses of the ATP2A1, ATP2A2 and ATP2A3 gene expression in MLO-Y4 osteocytic cells treated with the normal medium (NC), mannitol-supplemented medium (MAN, osmotic pressure-matched control), and high-glucose and high-fat (HGHF)-supplemented medium. d Western blotting analyses of the SERCA1, SERCA2, and SERCA3 protein expression in normal, mannitol-treated, and HGHF-treated MLO-Y4 osteocytic cells. e qRT-PCR analyses of the ATP2A1, ATP2A2 and ATP2A3 gene expression in male non-diabetic, KK-Ay, and HFD + STZ mice. f Immunohistochemical staining of SERCA1, SERCA2, and SERCA3 in osteocytes in the mouse tibiae of the non-diabetes, KK-Ay, and HFD + STZ groups. g Intracellular Ca2+ signaling in MLO-Y4 osteocytic cells in vitro treated with siCtrl and siSERCA2 in response to steady FSS stimulation at 2 Pa. Graphs represent mean ± SD (a, b, g: n = 120 cells per group; c–e n = 6 biologically independent replicates; f: n = 8 mice per group). ***P 

2). Neochlorogenic acid anchors MCU-based calcium overload for cancer therapy. Food & Function, 2021 (PubMed: 34672304) [IF=6.1]

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