RUFY1 Antibody - #DF4448
| Product: | RUFY1 Antibody |
| Catalog: | DF4448 |
| Description: | Rabbit polyclonal antibody to RUFY1 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Rabbit, Dog, Chicken |
| Mol.Wt.: | 80 KD; 80kD(Calculated). |
| Uniprot: | Q96T51 |
| RRID: | AB_2836803 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4448, RRID:AB_2836803.
Fold/Unfold
3000002E04Rik; FLJ22251; FYVE finger protein EIP1; FYVE-finger protein EIP1; La binding protein 1; La-binding protein 1; OTTHUMP00000161521; Rab4 interacting protein; Rab4-interacting protein; RABIP4; RUFY1; RUFY1_HUMAN; RUN and FYVE domain containing 1; RUN and FYVE domain-containing protein 1; RUN and FYVE domains containing protein 1; ZFYVE12; Zinc finger FYVE domain containing protein 12; Zinc finger FYVE domain-containing protein 12;
Immunogens
- Q96T51 RUFY1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADREGGCAAGRGRELEPELEPGPGPGSALEPGEEFEIVDRSQLPGPGDLRSATRPRAAEGWSAPILTLARRATGNLSASCGSALRAAAGLGGGDSGDGTARAASKCQMMEERANLMHMMKLSIKVLLQSALSLGRSLDADHAPLQQFFVVMEHCLKHGLKVKKSFIGQNKSFFGPLELVEKLCPEASDIATSVRNLPELKTAVGRGRAWLYLALMQKKLADYLKVLIDNKHLLSEFYEPEALMMEEEGMVIVGLLVGLNVLDANLCLKGEDLDSQVGVIDFSLYLKDVQDLDGGKEHERITDVLDQKNYVEELNRHLSCTVGDLQTKIDGLEKTNSKLQEELSAATDRICSLQEEQQQLREQNELIRERSEKSVEITKQDTKVELETYKQTRQGLDEMYSDVWKQLKEEKKVRLELEKELELQIGMKTEMEIAMKLLEKDTHEKQDTLVALRQQLEEVKAINLQMFHKAQNAESSLQQKNEAITSFEGKTNQVMSSMKQMEERLQHSERARQGAEERSHKLQQELGGRIGALQLQLSQLHEQCSSLEKELKSEKEQRQALQRELQHEKDTSSLLRMELQQVEGLKKELRELQDEKAELQKICEEQEQALQEMGLHLSQSKLKMEDIKEVNQALKGHAWLKDDEATHCRQCEKEFSISRRKHHCRNCGHIFCNTCSSNELALPSYPKPVRVCDSCHTLLLQRCSSTAS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96T51 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R12 | Methylation | Uniprot | |
| R14 | Methylation | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| T74 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Uniprot | |
| S83 | Phosphorylation | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| C184 | S-Nitrosylation | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| T192 | Phosphorylation | Uniprot | |
| S193 | Phosphorylation | Uniprot | |
| K225 | Methylation | Uniprot | |
| K308 | Ubiquitination | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| C320 | S-Nitrosylation | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| T347 | Phosphorylation | Uniprot | |
| R349 | Methylation | Uniprot | |
| S352 | Phosphorylation | Uniprot | |
| K379 | Acetylation | Uniprot | |
| Y389 | Phosphorylation | P51813 (BMX) | Uniprot |
| K390 | Ubiquitination | Uniprot | |
| Y400 | Phosphorylation | P51813 (BMX) | Uniprot |
| S401 | Phosphorylation | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| K460 | Ubiquitination | Uniprot | |
| K469 | Ubiquitination | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| S486 | Phosphorylation | Uniprot | |
| K490 | Ubiquitination | Uniprot | |
| K499 | Ubiquitination | Uniprot | |
| K521 | Ubiquitination | Uniprot | |
| K555 | Methylation | Uniprot | |
| K569 | Ubiquitination | Uniprot | |
| S618 | Phosphorylation | Uniprot | |
| S620 | Phosphorylation | Uniprot | |
| K621 | Ubiquitination | Uniprot | |
| K635 | Ubiquitination | Uniprot | |
| K641 | Ubiquitination | Uniprot | |
| S694 | Phosphorylation | Uniprot |
Research Backgrounds
Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.
Phosphorylation on Tyr-389 and/or Tyr-400 is required for interaction with BMX and endosomal targeting.
Cytoplasm. Early endosome membrane>Peripheral membrane protein.
Broadly expressed, with highest levels in lung, testis, kidney and brain.
Interacts with BMX. May interact with SSB. Interacts with RAB4 and RAB5 that have been activated by GTP-binding.
The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
Research Fields
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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