EWSR1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from HeLa cells using EWSR1 antibody.

Product:	EWSR1 Antibody
Catalog:	DF4447
Description:	Rabbit polyclonal antibody to EWSR1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.:	68 KD; 68kD(Calculated).
Uniprot:	Q01844
RRID:	AB_2836802

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(83%)

Clonality:

Polyclonal

Specificity:

EWSR1 Antibody detects endogenous levels of total EWSR1.

RRID:

AB_2836802
Cite Format: Affinity Biosciences Cat# DF4447, RRID:AB_2836802.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

bK984G1.4; bK984G1.4 Ewing sarcoma breakpoint region 1 protein; Ewing sarcoma breakpoint region 1; Ewing sarcoma breakpoint region 1 protein; Ewings sarcoma EWS Fli1 type 1 oncogene; EWS; EWS oncogene; EWS RNA binding protein 1; EWS_HUMAN; EWSR 1; Ewsr1; EWSR1 protein; RNA binding protein EWS; RNA-binding protein EWS;

Immunogens

Immunogen:

Uniprot:

Q01844(EWS_HUMAN) >>Visit HPA database.

Gene(ID):

EWSR1(2130)

Expression:

Q01844 EWS_HUMAN:

Ubiquitous.

Sequence:

Q01844 EWS_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYGQPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYSQAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQESGGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRGGRGGGRGGMGSAGERGGFNKPGGPMDEGPDLDLGPPVDPDEDSDNSAIYVQGLNDSVTLDDLADFFKQCGVVKMNKRTGQPMIHIYLDKETGKPKGDATVSYEDPPTAKAAVEWFDGKDFQGSKLKVSLARKKPPMNSMRGGLPPREGRGMPPPLRGGPGGPGGPGGPMGRMGGRGGDRGGFPPRGPRGSRGNPSGGGNVQHRAGDWQCPNPGCGNQNFAWRTECNQCKAPKPEGFLPPPFPPPGGDRGRGGPGGMRGGRGGLMDRGGPGGMFRGGRGGDRGGFRGGRGMDRGGFGGGRRGGPGGPPGPLMEQMGGRRGGRGGPGKMDKGEHRQERRDRPY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q01844 As Substrate

Q01844

Site	PTM Type	Enzyme	Source
T79	Phosphorylation	P45983 (MAPK8) , P28482 (MAPK1) , Q13535 (ATR) , Q13315 (ATM) , Q16539 (MAPK14) , Q15759 (MAPK11) , P27361 (MAPK3)	Uniprot
S266	Phosphorylation	P05771 (PRKCB) , P17252 (PRKCA)	Uniprot
Y278	Phosphorylation	P12931 (SRC)	Uniprot
S286	Phosphorylation		Uniprot
R292	Methylation		Uniprot
R300	Methylation		Uniprot
R302	Methylation		Uniprot
R304	Methylation		Uniprot
R309	Methylation		Uniprot
R314	Methylation		Uniprot
R317	Methylation		Uniprot
R321	Methylation		Uniprot
R330	Methylation		Uniprot
S371	Phosphorylation		Uniprot
Y417	Phosphorylation		Uniprot
T422	Phosphorylation		Uniprot
K424	Ubiquitination		Uniprot
K433	Acetylation		Uniprot
K433	Ubiquitination		Uniprot
K439	Acetylation		Uniprot
K439	Ubiquitination		Uniprot
S443	Phosphorylation		Uniprot
R455	Methylation		Uniprot
R461	Methylation		Uniprot
R464	Methylation		Uniprot
R471	Methylation		Uniprot
R486	Methylation		Uniprot
R490	Methylation		Uniprot
R494	Methylation		Uniprot
R500	Methylation		Uniprot
R503	Methylation		Uniprot
R506	Methylation		Uniprot
R563	Methylation		Uniprot
R565	Methylation		Uniprot
R572	Methylation		Uniprot
R575	Methylation		Uniprot
R581	Methylation		Uniprot
R589	Methylation		Uniprot
R592	Methylation		Uniprot
R596	Methylation		Uniprot
R600	Methylation		Uniprot
R603	Methylation		Uniprot
R607	Methylation		Uniprot
R614	Methylation		Uniprot
R615	Methylation		Uniprot
R632	Methylation		Uniprot
R633	Methylation		Uniprot
R636	Methylation		Uniprot
Y656	Phosphorylation		Uniprot

Research Backgrounds

Q01844_EWS_HUMAN

Function:

Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

PTMs:

Phosphorylated; calmodulin-binding inhibits phosphorylation of Ser-266.

Highly methylated on arginine residues. Methylation is mediated by PRMT1 and, at lower level by PRMT8.

Subcellular Location:

Nucleus. Cytoplasm. Cell membrane.
Note: Relocates from cytoplasm to ribosomes upon PTK2B/FAK2 activation.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Binds POLR2C, SF1, calmodulin and RNA. Interacts with PTK2B/FAK2 and TDRD3. Binds calmodulin in the presence, but not in the absence, of calcium ion. Forms a complex with REC8, PRDM9, SYCP3 and SYCP1; complex formation is dependent of phosphorylated form of REC8 and requires PRDM9 bound to hotspot DNA; EWSR1 joins PRDM9 with the chromosomal axis through REC8 (By similarity).

Family&Domains:

EWS activation domain (EAD) functions as a potent activation domain in EFPS. EWSR1 binds POLR2C but not POLR2E or POLR2G, whereas the isolated EAD binds POLR2E and POLR2G but not POLR2C. Cis-linked RNA-binding domain (RBD) can strongly and specifically repress trans-activation by the EAD.

Belongs to the RRM TET family.

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

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EWSR1 Antibody - #DF4447