ARHGEF12 Antibody | Affinity Biosciences

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FIGURE 4 | Reducing expressions of PLCβ and RhoGEF12 of neoliensinine in mesenteric arteries contracted by KCl.

Figure 3 Effect of GS on regulating miR-196-5p and miR-361-5p expression.

Product:	ARHGEF12 Antibody
Catalog:	DF4432
Description:	Rabbit polyclonal antibody to ARHGEF12
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	173 KD; 173kD(Calculated).
Uniprot:	Q9NZN5
RRID:	AB_2836787

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%)

Clonality:

Polyclonal

Specificity:

ARHGEF12 Antibody detects endogenous levels of total ARHGEF12.

RRID:

AB_2836787
Cite Format: Affinity Biosciences Cat# DF4432, RRID:AB_2836787.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ARHGC_HUMAN; ARHGEF12; KIAA0382; leukemia-associated rho guanine nucleotide exchange factor; Leukemia-associated RhoGEF; PRO2792; Rho guanine exchange factor (GEF) 12; Rho guanine nucleotide exchange factor (GEF) 12; Rho guanine nucleotide exchange factor 12; Similar to Mouse Lsc oncogene;

Immunogens

Immunogen:

Uniprot:

Q9NZN5(ARHGC_HUMAN) >>Visit HPA database.

Gene(ID):

ARHGEF12(23365)

Expression:

Q9NZN5 ARHGC_HUMAN:

Ubiquitously expressed. Isoform 2 is found in jejunum and testis.

Sequence:

Q9NZN5 ARHGC_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERIASHDFDPTDSSSKKTKSSSEESRSEIYGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDASRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NZN5 As Substrate

Q9NZN5

Site	PTM Type	Source
S2	Acetylation	Uniprot
S2	Phosphorylation	Uniprot
T9	Phosphorylation	Uniprot
R11	Methylation	Uniprot
S22	Phosphorylation	Uniprot
S28	Phosphorylation	Uniprot
T30	Phosphorylation	Uniprot
K32	Acetylation	Uniprot
S41	Phosphorylation	Uniprot
Y66	Phosphorylation	Uniprot
S178	Phosphorylation	Uniprot
S190	Phosphorylation	Uniprot
K205	Ubiquitination	Uniprot
C294	S-Nitrosylation	Uniprot
S296	Phosphorylation	Uniprot
S304	Phosphorylation	Uniprot
S309	Phosphorylation	Uniprot
S312	Phosphorylation	Uniprot
S337	Phosphorylation	Uniprot
S341	Phosphorylation	Uniprot
T532	Phosphorylation	Uniprot
Y546	Phosphorylation	Uniprot
K591	Acetylation	Uniprot
K593	Acetylation	Uniprot
S599	Phosphorylation	Uniprot
S608	Phosphorylation	Uniprot
S613	Phosphorylation	Uniprot
R617	Methylation	Uniprot
T632	Phosphorylation	Uniprot
S634	Phosphorylation	Uniprot
S635	Phosphorylation	Uniprot
S637	Phosphorylation	Uniprot
Y660	Phosphorylation	Uniprot
T699	Phosphorylation	Uniprot
T703	Phosphorylation	Uniprot
T732	Phosphorylation	Uniprot
T736	Phosphorylation	Uniprot
S753	Phosphorylation	Uniprot
S818	Phosphorylation	Uniprot
S824	Phosphorylation	Uniprot
S826	Phosphorylation	Uniprot
R829	Methylation	Uniprot
C921	S-Nitrosylation	Uniprot
K1000	Ubiquitination	Uniprot
Y1004	Phosphorylation	Uniprot
K1017	Ubiquitination	Uniprot
K1064	Ubiquitination	Uniprot
S1072	Phosphorylation	Uniprot
S1170	Phosphorylation	Uniprot
S1176	Phosphorylation	Uniprot
S1203	Phosphorylation	Uniprot
Y1232	Phosphorylation	Uniprot
S1239	Phosphorylation	Uniprot
S1273	Phosphorylation	Uniprot
Y1284	Phosphorylation	Uniprot
T1286	Phosphorylation	Uniprot
S1288	Phosphorylation	Uniprot
T1293	Phosphorylation	Uniprot
S1295	Phosphorylation	Uniprot
S1308	Phosphorylation	Uniprot
T1324	Phosphorylation	Uniprot
Y1326	Phosphorylation	Uniprot
S1327	Phosphorylation	Uniprot
S1331	Phosphorylation	Uniprot
S1377	Phosphorylation	Uniprot
S1389	Phosphorylation	Uniprot
Y1415	Phosphorylation	Uniprot
S1457	Phosphorylation	Uniprot
S1463	Phosphorylation	Uniprot
S1492	Phosphorylation	Uniprot
Y1524	Phosphorylation	Uniprot
T1525	Phosphorylation	Uniprot
S1541	Phosphorylation	Uniprot
S1544	Phosphorylation	Uniprot

Research Backgrounds

Q9NZN5_ARHGC_HUMAN

Function:

May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.

Subcellular Location:

Cytoplasm. Membrane.
Note: Translocated to the membrane upon stimulation.

Tissue Specificity:

Ubiquitously expressed. Isoform 2 is found in jejunum and testis.

Subunit Structure:

Interacts with GNA12 and GNA13, probably through the RGS-like domain. Interacts with RHOA, PLXNB1 and PLXNB2. Interacts through its PDZ domain with IGF1R beta subunit. Interacts with GCSAM.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)

· Organismal Systems > Development > Axon guidance. (View pathway)

· Organismal Systems > Immune system > Platelet activation. (View pathway)

References

1). ITRAQ-Based Proteomics Analysis Reveals the Effect of Neoliensinine on KCl-Induced Vascular Smooth Muscle Contraction by Inhibiting Regulatory Light Chain Phosphorylation. Frontiers in Pharmacology, 2019 (PubMed: 31572175) [IF=5.6]

Application: WB Species: mouse Sample: mesenteric arteries

FIGURE 4 | Reducing expressions of PLCβ and RhoGEF12 of neoliensinine in mesenteric arteries contracted by KCl. (A) Protein expressions of PLCβ and RhoGEF12 by Western blot

2). MiR-361-5p/abca1 and MiR-196-5p/arhgef12 Axis Involved in γ-Sitosterol Inducing Dual Anti-Proliferative Effects on Bronchial Epithelial Cells of Chronic Obstructive Pulmonary Disease. International Journal of Chronic Obstructive Pulmonary Disease, 2021 (PubMed: 34675500) [IF=2.8]

Application: WB Species: Human Sample: H292 and BEAS-2B cells

Figure 3 Effect of GS on regulating miR-196-5p and miR-361-5p expression. (A) After GS treatment, the expressions of miR-196-5p and miR-361-5p were detected in BEAS-2B and H292 cells. (B) Cell proliferation were performed in BEAS-2B cells after GS single or combined with either miR-196-5p mimic or miR-361-5p inhibitor. (C) miR-196-5p and miR-361-5p target mRNAs were matched to GSE130928 profile, respectively. The expressions of bcat1 and arhgef12 of GSE130928 were showed in (D) and the binding motifs of miR-196-5p to arhgef12 and miR-361-5p to bcat1 were showed in (E), respectively. The mRNA (F) and protein levels (G) were detected in H292 and BEAS-2B cells. Shown are representative and expressed as the Means ± S.D. The bars with different superscripts in each panel were significantly different. Experiments were performed in triplicate for each group, *p< 0.05.

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ARHGEF12 Antibody - #DF4432