ARHGEF11 Antibody - #DF4431

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Product: ARHGEF11 Antibody
Catalog: DF4431
Description: Rabbit polyclonal antibody to ARHGEF11
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 170 KD; 168kD(Calculated).
Uniprot: O15085
RRID: AB_2836786

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Sheep(83%), Rabbit(100%), Dog(92%)
Clonality:
Polyclonal
Specificity:
ARHGEF11 Antibody detects endogenous levels of total ARHGEF11.
RRID:
AB_2836786
Cite Format: Affinity Biosciences Cat# DF4431, RRID:AB_2836786.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARHGB_HUMAN; ARHGEF 11; ARHGEF11; DKFZp667F1223; Glutamate transporter EAAT4 associated protein 48; GTRAP 48; GTRAP48; KIAA0380; OTTHUMP00000038716; OTTHUMP00000038717; PDZ RHOGEF; PDZ-RhoGEF; Rho guanine exchange factor (GEF) 11; Rho guanine nucleotide exchange factor (GEF) 11; Rho guanine nucleotide exchange factor 11; RhoA specific guanine nucleotide exchange factor; RhoGEF glutamate transport modulator;

Immunogens

Immunogen:
Uniprot:

O15085(ARHGB_HUMAN) >>Visit HPA database.

Gene(ID):
ARHGEF11(9826)
Expression:
O15085 ARHGB_HUMAN:

Ubiquitously expressed.

Sequence:
MSVRLPQSIDRLSSLSSLGDSAPERKSPSHHRQPSDASETTGLVQRCVIIQKDQHGFGFTVSGDRIVLVQSVRPGGAAMKAGVKEGDRIIKVNGTMVTNSSHLEVVKLIKSGAYVALTLLGSSPSSMGISGLQQDPSPAGAPRITSVIPSPPPPPPLPPPQRITGPKPLQDPEVQKHATQILRNMLRQEEKELQDILPLYGDTSQRPSEGRLSLDSQEGDSGLDSGTERFPSLSESLMNRNSVLSDPGLDSPRTSPVIMARVAQHHRRQGSDAAVPSTGDQGVDQSPKPLIIGPEEDYDPGYFNNESDIIFQDLEKLKSRPAHLGVFLRYIFSQADPSPLLFYLCAEVYQQASPKDSRSLGKDIWNIFLEKNAPLRVKIPEMLQAEIDSRLRNSEDARGVLCEAQEAAMPEIQEQIHDYRTKRTLGLGSLYGENDLLDLDGDPLRERQVAEKQLAALGDILSKYEEDRSAPMDFALNTYMSHAGIRLREARPSNTAEKAQSAPDKDKWLPFFPKTKKSSNSKKEKDALEDKKRNPILKYIGKPKSSSQSTFHIPLSPVEVKPGNVRNIIQHFENNQQYDAPEPGTQRLSTGSFPEDLLESDSSRSEIRLGRSESLKGREEMKRSRKAENVPRSRSDVDMDAAAEATRLHQSASSSTSSLSTRSLENPTPPFTPKMGRRSIESPSLGFCTDTLLPHLLEDDLGQLSDLEPEPDAQNWQHTVGKDVVAGLTQREIDRQEVINELFVTEASHLRTLRVLDLIFYQRMKKENLMPREELARLFPNLPELIEIHNSWCEAMKKLREEGPIIKEISDLMLARFDGPAREELQQVAAQFCSYQSIALELIKTKQRKESRFQLFMQEAESHPQCRRLQLRDLIISEMQRLTKYPLLLESIIKHTEGGTSEHEKLCRARDQCREILKYVNEAVKQTENRHRLEGYQKRLDATALERASNPLAAEFKSLDLTTRKMIHEGPLTWRISKDKTLDLHVLLLEDLLVLLQKQDEKLLLKCHSKTAVGSSDSKQTFSPVLKLNAVLIRSVATDKRAFFIICTSKLGPPQIYELVALTSSDKNTWMELLEEAVRNATRHPGAAPMPVHPPPPGPREPAQQGPTPSRVELDDSDVFHGEPEPEELPGGTGSQQRVQGKHQVLLEDPEQEGSAEEEELGVLPCPSTSLDGENRGIRTRNPIHLAFPGPLFMEGLADSALEDVENLRHLILWSLLPGHTMETQAAQEPEDDLTPTPSVISVTSHPWDPGSPGQAPPGGEGDNTQLAGLEGERPEQEDMGLCSLEHLPPRTRNSGIWESPELDRNLAEDASSTEAAGGYKVVRKAEVAGSKVVPALPESGQSEPGPPEVEGGTKATGNCFYVSMPSGPPDSSTDHSEAPMSPPQPDSLPAGQTEPQPQLQGGNDDPRRPSRSPPSLALRDVGMIFHTIEQLTLKLNRLKDMELAHRELLKSLGGESSGGTTPVGSFHTEAARWTDGSLSPPAKEPLASDSRNSHELGPCPEDGSDAPLEDSTADAAASPGP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Rabbit
100
Dog
92
Sheep
83
Bovine
75
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15085 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
S8 Phosphorylation
S14 Phosphorylation
S16 Phosphorylation
S27 Phosphorylation
S29 Phosphorylation
S35 Phosphorylation
S38 Phosphorylation
T40 Phosphorylation
T41 Phosphorylation
S150 Phosphorylation
S213 Phosphorylation
S216 Phosphorylation
S232 Phosphorylation
S242 Phosphorylation
S245 Phosphorylation
S251 Phosphorylation
T254 Phosphorylation
S255 Phosphorylation
S271 Phosphorylation
S359 Phosphorylation
S394 Phosphorylation
T421 Phosphorylation
S429 Phosphorylation
S556 Phosphorylation
S589 Phosphorylation
T590 Phosphorylation
S592 Phosphorylation
S614 Phosphorylation
S633 Phosphorylation
S635 Phosphorylation
S651 Phosphorylation
S653 Phosphorylation
S654 Phosphorylation
S655 Phosphorylation
T656 Phosphorylation
S657 Phosphorylation
S658 Phosphorylation
S663 Phosphorylation
T668 Phosphorylation
T672 Phosphorylation
S679 Phosphorylation
S851 Phosphorylation
K925 Acetylation
K938 Ubiquitination
T1063 Phosphorylation
S1064 Phosphorylation
S1065 Phosphorylation
S1135 Phosphorylation
S1155 Phosphorylation
S1168 Phosphorylation
T1292 Phosphorylation
S1295 Phosphorylation
S1300 Phosphorylation
S1411 Phosphorylation
S1413 Phosphorylation
S1452 Phosphorylation
S1457 Phosphorylation
S1458 Phosphorylation
T1461 Phosphorylation
T1462 Phosphorylation
S1466 Phosphorylation
T1475 Phosphorylation
S1478 Phosphorylation
S1480 Phosphorylation
S1489 Phosphorylation
S1491 Phosphorylation
C1500 S-Nitrosylation
S1519 Phosphorylation

Research Backgrounds

Function:

May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. Involved in neurotrophin-induced neurite outgrowth.

PTMs:

Phosphorylated by MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14).

Ubiquitinated by the BCR(KLHL20) E3 ubiquitin ligase complex when previously phosphorylated by MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14), leading to its degradation, thereby restricting RhoA activity and facilitating growth cone spreading and neurite outgrowth.

Subcellular Location:

Cytoplasm. Membrane.
Note: Translocated to the membrane upon stimulation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Interacts with GNA12 and GNA13 through the RGS domain. Interacts with RHOA, PLXNB1 and PLXNB2. Interacts with SLC1A6 (By similarity). Interacts (via DH domain) with GCSAM (via C-terminus).

Family&Domains:

The poly-Pro region is essential for plasma membrane localization upon stimulation.

Research Fields

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

Restrictive clause

 

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