ARHGEF1 Antibody - #DF4429

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Product: ARHGEF1 Antibody
Catalog: DF4429
Description: Rabbit polyclonal antibody to ARHGEF1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Dog
Mol.Wt.: 105 KD; 102kD(Calculated).
Uniprot: Q92888
RRID: AB_2836784

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(85%), Bovine(92%), Sheep(92%), Dog(85%)
Clonality:
Polyclonal
Specificity:
ARHGEF1 Antibody detects endogenous levels of total ARHGEF1.
RRID:
AB_2836784
Cite Format: Affinity Biosciences Cat# DF4429, RRID:AB_2836784.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

115 kD protein; 115 kDa guanine nucleotide exchange factor; ARHG1_HUMAN; ARHGEF 1; ARHGEF1; GEF 1; GEF1; LBCL 2; LBCL2; LSC; Lsc homolog; p115 RhoGEF; p115-RhoGEF; p115RhoGEF; Rho guanine nucleotide exchange factor (GEF) 1; Rho guanine nucleotide exchange factor 1; Sub1.5;

Immunogens

Immunogen:
Uniprot:

Q92888(ARHG1_HUMAN) >>Visit HPA database.

Gene(ID):
ARHGEF1(9138)
Expression:
Q92888 ARHG1_HUMAN:

Ubiquitously expressed.

Sequence:
MEDFARGAASPGPSRPGLVPVSIIGAEDEDFENELETNSEEQNSQFQSLEQVKRRPAHLMALLQHVALQFEPGPLLCCLHADMLGSLGPKEAKKAFLDFYHSFLEKTAVLRVPVPPNVAFELDRTRADLISEDVQRRFVQEVVQSQQVAVGRQLEDFRSKRLMGMTPWEQELAQLEAWVGRDRASYEARERHVAERLLMHLEEMQHTISTDEEKSAAVVNAIGLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPAKTKKGLSSILDAARWNRGEPQVPDFRHLKAEVDAEKPGATDRKGGVGMPSRDRNIGAPGQDTPGVSLHPLSLDSPDREPGADAPLELGDSSPQGPMSLESLAPPESTDEGAETESPEPGDEGEPGRSGLELEPEEPPGWRELVPPDTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFFQPMAECLFFPLEELQNIFPSLDELIEVHSLFLDRLMKRRQESGYLIEEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEEPTEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLSHLRQSSDPMLSEFKNLDITKKKLVHEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERLLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCALITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGRETSPADARTERILSDLLPFCRPGPEGQLAATALRKVLSLKQLLFPAEEDNGAGPPRDGDGVPGGGPLSPARTQEIQENLLSLEETMKQLEELEEEFCRLRPLLSQLGGNSVPQPGCT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
92
Sheep
92
Pig
85
Dog
85
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92888 As Substrate

Site PTM Type Enzyme
S10 Phosphorylation
S14 Phosphorylation
S102 Phosphorylation
K234 Acetylation
S240 Phosphorylation
S255 Phosphorylation
K263 Ubiquitination
S266 Phosphorylation
S267 Phosphorylation
K288 Ubiquitination
S325 Phosphorylation
S330 Phosphorylation
S350 Phosphorylation
S374 Phosphorylation
S409 Phosphorylation
K412 Ubiquitination
Y487 Phosphorylation
K510 Ubiquitination
K527 Ubiquitination
K555 Ubiquitination
K567 Ubiquitination
K587 Ubiquitination
S625 Phosphorylation
S630 Phosphorylation
S631 Phosphorylation
K639 Ubiquitination
K647 Ubiquitination
K688 Ubiquitination
T695 Phosphorylation
K701 Ubiquitination
Y738 Phosphorylation O60674 (JAK2)
K763 Ubiquitination
S776 Phosphorylation
S786 Phosphorylation
S788 Phosphorylation
S798 Phosphorylation
S809 Phosphorylation
S833 Phosphorylation
K835 Ubiquitination
S863 Phosphorylation
S876 Phosphorylation
T912 Phosphorylation

Research Backgrounds

Function:

Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.

PTMs:

Phosphorylated by PKCA. Angiotensin-2 induced Tyr-738 phosphorylation is mediated by JAK2.

Subcellular Location:

Cytoplasm. Membrane.
Note: Translocated to the membrane by activated GNA13 or LPA stimulation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Interacts with RHOA, GNA12 and GNA13. Homooligomerizes through the coiled coil region. May interact with CCPG1 (By similarity). Interacts with CTNNAL1.

Family&Domains:

The RGSL domain, also known as rgRGS domain, is necessary but not sufficient for GAP activity.

The DH domain is involved in interaction with CCPG1.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

Restrictive clause

 

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For Research Use Only.
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