RHG9 Antibody - #DF4428

(3)  
Product: RHG9 Antibody
Catalog: DF4428
Description: Rabbit polyclonal antibody to RHG9
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep
Mol.Wt.: 90 KD; 83kD(Calculated).
Uniprot: Q9BRR9
RRID: AB_2836783

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(82%), Bovine(91%), Horse(100%), Sheep(91%)
Clonality:
Polyclonal
Specificity:
RHG9 Antibody detects endogenous levels of total RHG9.
RRID:
AB_2836783
Cite Format: Affinity Biosciences Cat# DF4428, RRID:AB_2836783.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

10C; ARHGAP9; FLJ16525; MGC1295; RGL1; RHG09_HUMAN; Rho GTPase activating protein 9; Rho GTPase-activating protein 9; Rho type GTPase activating protein 9; Rho-type GTPase-activating protein 9;

Immunogens

Immunogen:
Uniprot:

Q9BRR9(RHG09_HUMAN) >>Visit HPA database.

Gene(ID):
ARHGAP9(64333)
Expression:
Q9BRR9 RHG09_HUMAN:

Predominantly expressed in peripheral blood leukocytes, spleen, and thymus.

Sequence:
MLSSRWWPSSWGILGLGPRSPPRGSQLCALYAFTYTGADGQQVSLAEGDRFLLLRKTNSDWWLARRLEAPSTSRPIFVPAAYMIEESIPSQSPTTVIPGQLLWTPGPKLFHGSLEELSQALPSRAQASSEQPPPLPRKMCRSVSTDNLSPSLLKPFQEGPSGRSLSQEDLPSEASASTAGPQPLMSEPPVYCNLVDLRRCPRSPPPGPACPLLQRLDAWEQHLDPNSGRCFYINSLTGCKSWKPPRRSRSETNPGSMEGTQTLKRNNDVLQPQAKGFRSDTGTPEPLDPQGSLSLSQRTSQLDPPALQAPRPLPQLLDDPHEVEKSGLLNMTKIAQGGRKLRKNWGPSWVVLTGNSLVFYREPPPTAPSSGWGPAGSRPESSVDLRGAALAHGRHLSSRRNVLHIRTIPGHEFLLQSDHETELRAWHRALRTVIERLVRWVEARREAPTGRDQGSGDRENPLELRLSGSGPAELSAGEDEEEESELVSKPLLRLSSRRSSIRGPEGTEQNRVRNKLKRLIAKRPPLQSLQERGLLRDQVFGCQLESLCQREGDTVPSFLRLCIAAVDKRGLDVDGIYRVSGNLAVVQKLRFLVDRERAVTSDGRYVFPEQPGQEGRLDLDSTEWDDIHVVTGALKLFLRELPQPLVPPLLLPHFRAALALSESEQCLSQIQELIGSMPKPNHDTLRYLLEHLCRVIAHSDKNRMTPHNLGIVFGPTLFRPEQETSDPAAHALYPGQLVQLMLTNFTSLFP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
91
Sheep
91
Pig
82
Rabbit
75
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BRR9 As Substrate

Site PTM Type Enzyme
S20 Phosphorylation
K56 Ubiquitination
S113 Phosphorylation
S123 Phosphorylation
S128 Phosphorylation
S144 Phosphorylation
S161 Phosphorylation
S166 Phosphorylation
T178 Phosphorylation
S186 Phosphorylation
Y191 Phosphorylation
S203 Phosphorylation
Y232 Phosphorylation
K264 Ubiquitination
K275 Ubiquitination
S279 Phosphorylation
T283 Phosphorylation
S296 Phosphorylation
K325 Ubiquitination
K333 Ubiquitination
R386 Methylation
S417 Phosphorylation
S467 Phosphorylation
S469 Phosphorylation
S475 Phosphorylation
S484 Phosphorylation
S488 Phosphorylation
K489 Ubiquitination
S495 Phosphorylation
S496 Phosphorylation
S499 Phosphorylation
S500 Phosphorylation
K522 Ubiquitination
K568 Ubiquitination
K588 Ubiquitination

Research Backgrounds

Function:

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has a substantial GAP activity toward CDC42 and RAC1 and less toward RHOA. Has a role in regulating adhesion of hematopoietic cells to the extracellular matrix. Binds phosphoinositides, and has the highest affinity for phosphatidylinositol 3,4,5-trisphosphate, followed by phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 4,5-bisphosphate.

Tissue Specificity:

Predominantly expressed in peripheral blood leukocytes, spleen, and thymus.

Subunit Structure:

Interacts with FASLG.

Family&Domains:

A region including the PH domain and partially overlapping with the Rho-GAP domain mediates interaction with phosphoinositides.

Restrictive clause

 

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