RHG17 Antibody - #DF4426

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Product: RHG17 Antibody
Catalog: DF4426
Description: Rabbit polyclonal antibody to RHG17
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.: 100 KD; 95kD(Calculated).
Uniprot: Q68EM7
RRID: AB_2836781

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Rabbit(83%), Dog(100%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
RHG17 Antibody detects endogenous levels of total RHG17.
RRID:
AB_2836781
Cite Format: Affinity Biosciences Cat# DF4426, RRID:AB_2836781.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MST066; MST110; MSTP038; MSTP066; MSTP110; NADRIN; Neuron associated developmentally regulated protein; PP367; PP4534; Rho GTPase activating protein 17; Rho type GTPase activating protein 17; RhoGAP interacting with CIP4 homologs 1; RhoGAP interacting with CIP4 homologs protein 1; RICH 1; RICH1; RICH1B; WBP15;

Immunogens

Immunogen:
Uniprot:

Q68EM7(RHG17_HUMAN) >>Visit HPA database.

Gene(ID):
ARHGAP17(55114)
Expression:
Q68EM7 RHG17_HUMAN:

Ubiquitously expressed. Expressed at higher level in heart and placenta.

Sequence:
MKKQFNRMKQLANQTVGRAEKTEVLSEDLLQIERRLDTVRSICHHSHKRLVACFQGQHGTDAERRHKKLPLTALAQNMQEASTQLEDSLLGKMLETCGDAENQLALELSQHEVFVEKEIVDPLYGIAEVEIPNIQKQRKQLARLVLDWDSVRARWNQAHKSSGTNFQGLPSKIDTLKEEMDEAGNKVEQCKDQLAADMYNFMAKEGEYGKFFVTLLEAQADYHRKALAVLEKTLPEMRAHQDKWAEKPAFGTPLEEHLKRSGREIALPIEACVMLLLETGMKEEGLFRIGAGASKLKKLKAALDCSTSHLDEFYSDPHAVAGALKSYLRELPEPLMTFNLYEEWTQVASVQDQDKKLQDLWRTCQKLPPQNFVNFRYLIKFLAKLAQTSDVNKMTPSNIAIVLGPNLLWARNEGTLAEMAAATSVHVVAVIEPIIQHADWFFPEEVEFNVSEAFVPLTTPSSNHSFHTGNDSDSGTLERKRPASMAVMEGDLVKKESFGVKLMDFQAHRRGGTLNRKHISPAFQPPLPPTDGSTVVPAGPEPPPQSSRAESSSGGGTVPSSAGILEQGPSPGDGSPPKPKDPVSAAVPAPGRNNSQIASGQNQPQAAAGSHQLSMGQPHNAAGPSPHTLRRAVKKPAPAPPKPGNPPPGHPGGQSSSGTSQHPPSLSPKPPTRSPSPPTQHTGQPPGQPSAPSQLSAPRRYSSSLSPIQAPNHPPPQPPTQATPLMHTKPNSQGPPNPMALPSEHGLEQPSHTPPQTPTPPSTPPLGKQNPSLPAPQTLAGGNPETAQPHAGTLPRPRPVPKPRNRPSVPPPPQPPGVHSAGDSSLTNTAPTASKIVTDSNSRVSEPHRSIFPEMHSDSASKDVPGRILLDIDNDTESTAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Rabbit
83
Xenopus
80
Sheep
67
Pig
63
Chicken
45
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q68EM7 As Substrate

Site PTM Type Enzyme
K3 Methylation
K9 Ubiquitination
K21 Ubiquitination
K48 Acetylation
T72 Phosphorylation
S82 Phosphorylation
T83 Phosphorylation
Y124 Phosphorylation
K136 Ubiquitination
K160 Ubiquitination
S161 Phosphorylation
S162 Phosphorylation
K172 Ubiquitination
K191 Ubiquitination
K204 Ubiquitination
K232 Ubiquitination
K243 Ubiquitination
K247 Ubiquitination
K295 Acetylation
K295 Ubiquitination
Y314 Phosphorylation
K325 Ubiquitination
Y377 Phosphorylation
K384 Ubiquitination
S474 Phosphorylation
S484 Phosphorylation
S497 Phosphorylation
S520 Phosphorylation
S560 Phosphorylation
S570 Phosphorylation
S575 Phosphorylation
S584 Phosphorylation
S595 Phosphorylation
S599 Phosphorylation
S610 Phosphorylation
S614 Phosphorylation
S625 Phosphorylation
T628 Phosphorylation
S667 Phosphorylation
T672 Phosphorylation
S674 Phosphorylation
S676 Phosphorylation
T679 Phosphorylation
T682 Phosphorylation
S690 Phosphorylation
S696 Phosphorylation
S702 Phosphorylation P17612 (PRKACA)
S703 Phosphorylation
S704 Phosphorylation
S706 Phosphorylation
S751 Phosphorylation
T753 Phosphorylation
T757 Phosphorylation
T759 Phosphorylation
S762 Phosphorylation
T763 Phosphorylation
T793 Phosphorylation
S808 Phosphorylation
S857 Phosphorylation

Research Backgrounds

Function:

Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. Specifically acts as a GTPase activator for the CDC42 GTPase by converting it to an inactive GDP-bound state. The complex formed with AMOT acts by regulating the uptake of polarity proteins at tight junctions, possibly by deciding whether tight junction transmembrane proteins are recycled back to the plasma membrane or sent elsewhere. Participates in the Ca(2+)-dependent regulation of exocytosis, possibly by catalyzing GTPase activity of Rho family proteins and by inducing the reorganization of the cortical actin filaments. Acts as a GTPase activator in vitro for RAC1.

Subcellular Location:

Membrane>Peripheral membrane protein. Cytoplasm. Cell junction>Tight junction.
Note: Associates with membranes and concentrates at sites of cell-cell contact.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed. Expressed at higher level in heart and placenta.

Subunit Structure:

Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, PATJ and PARD3/PAR3. Interacts with SLC9A3R1, FNBP1, TRIP10, CAPZA (CAPZA1, CAPZA2 or CAPZA3), CAPZB, CD2AP and SH3KBP1/CIN85.

Family&Domains:

The BAR domain mediates the interaction with the coiled coil domain of AMOT, leading to its recruitment to tight junctions.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

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