ARHGAP11A Antibody - #DF4425

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Product: ARHGAP11A Antibody
Catalog: DF4425
Description: Rabbit polyclonal antibody to ARHGAP11A
Application: WB IF/ICC
Reactivity: Human, Mouse
Mol.Wt.: 110 KD; 114kD(Calculated).
Uniprot: Q6P4F7
RRID: AB_2836780

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
ARHGAP11A Antibody detects endogenous levels of total ARHGAP11A.
RRID:
AB_2836780
Cite Format: Affinity Biosciences Cat# DF4425, RRID:AB_2836780.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARHGAP11A; GAP (1-12); RHG11A; RHGBA; RHGBA_HUMAN; Rho GTPase-activating protein 11A; Rho-type GTPase-activating protein 11A;

Immunogens

Immunogen:
Uniprot:

Q6P4F7(RHGBA_HUMAN) >>Visit HPA database.

Gene(ID):
ARHGAP11A(9824)
Sequence:
MWDQRLVRLALLQHLRAFYGIKVKGVRGQCDRRRHETAATEIGGKIFGVPFNALPHSAVPEYGHIPSFLVDACTSLEDHIHTEGLFRKSGSVIRLKALKNKVDHGEGCLSSAPPCDIAGLLKQFFRELPEPILPADLHEALLKAQQLGTEEKNKATLLLSCLLADHTVHVLRYFFNFLRNVSLRSSENKMDSSNLAVIFAPNLLQTSEGHEKMSSNTEKKLRLQAAVVQTLIDYASDIGRVPDFILEKIPAMLGIDGLCATPSLEGFEEGEYETPGEYKRKRRQSVGDFVSGALNKFKPNRTPSITPQEERIAQLSESPVILTPNAKRTLPVDSSHGFSSKKRKSIKHNFNFELLPSNLFNSSSTPVSVHIDTSSEGSSQSSLSPVLIGGNHLITAGVPRRSKRIAGKKVCRVESGKAGCFSPKISHKEKVRRSLRLKFNLGKNGREVNGCSGVNRYESVGWRLANQQSLKNRIESVKTGLLFSPDVDEKLPKKGSEKISKSEETLLTPERLVGTNYRMSWTGPNNSSFQEVDANEASSMVENLEVENSLEPDIMVEKSPATSCELTPSNLNNKHNSNITSSPLSGDENNMTKETLVKVQKAFSESGSNLHALMNQRQSSVTNVGKVKLTEPSYLEDSPEENLFETNDLTIVESKEKYEHHTGKGEKCFSERDFSPLQTQTFNRETTIKCYSTQMKMEHEKDIHSNMPKDYLSKQEFSSDEEIKKQQSPKDKLNNKLKENENMMEGNLPKCAAHSKDEARSSFSQQSTCVVTNLSKPRPMRIAKQQSLETCEKTVSESSQMTEHRKVSDHIQWFNKLSLNEPNRIKVKSPLKFQRTPVRQSVRRINSLLEYSRQPTGHKLASLGDTASPLVKSVSCDGALSSCIESASKDSSVSCIKSGPKEQKSMSCEESNIGAISKSSMELPSKSFLKMRKHPDSVNASLRSTTVYKQKILSDGQVKVPLDDLTNHDIVKPVVNNNMGISSGINNRVLRRPSERGRAWYKGSPKHPIGKTQLLPTSKPVDL

PTMs - Q6P4F7 As Substrate

Site PTM Type Enzyme
K143 Ubiquitination
T261 Phosphorylation
S263 Phosphorylation
T274 Phosphorylation
S285 Phosphorylation
S291 Phosphorylation
T302 Phosphorylation
S304 Phosphorylation
T306 Phosphorylation
S316 Phosphorylation
S318 Phosphorylation
T323 Phosphorylation
K327 Ubiquitination
T329 Phosphorylation
S334 Phosphorylation
S335 Phosphorylation
S339 Phosphorylation
S340 Phosphorylation
K341 Ubiquitination
S415 Phosphorylation
K417 Ubiquitination
S422 Phosphorylation
S426 Phosphorylation
S434 Phosphorylation
K438 Ubiquitination
S452 Phosphorylation
Y457 Phosphorylation
S469 Phosphorylation
K471 Ubiquitination
K478 Ubiquitination
S484 Phosphorylation
K490 Ubiquitination
K501 Ubiquitination
T508 Phosphorylation
S559 Phosphorylation
T562 Phosphorylation
S563 Phosphorylation
T567 Phosphorylation
S569 Phosphorylation
S577 Phosphorylation
S581 Phosphorylation
S582 Phosphorylation
S585 Phosphorylation
K601 Ubiquitination
S604 Phosphorylation
S606 Phosphorylation
S620 Phosphorylation
K626 Ubiquitination
S638 Phosphorylation
K664 Ubiquitination
S670 Phosphorylation
S675 Phosphorylation
K714 Ubiquitination
S718 Phosphorylation
S719 Phosphorylation
S728 Phosphorylation
K730 Acetylation
K732 Acetylation
S787 Phosphorylation
S796 Phosphorylation
K816 Ubiquitination
S829 Phosphorylation
T836 Phosphorylation
S847 Phosphorylation
S862 Phosphorylation
T866 Phosphorylation
S868 Phosphorylation
S875 Phosphorylation
S882 Phosphorylation
S886 Phosphorylation
S891 Phosphorylation
S917 Phosphorylation
S925 Phosphorylation
K926 Ubiquitination
K930 Methylation
S937 Phosphorylation
S941 Phosphorylation
K951 Ubiquitination
Y1001 Phosphorylation
S1004 Phosphorylation
K1019 Acetylation

Research Backgrounds

References

1). ARHGAP11A Promotes the Malignant Progression of Gastric Cancer by Regulating the Stability of Actin Filaments through TPM1. Journal of Oncology, 2021 (PubMed: 34912455)

Application: WB    Species: Mice    Sample: gastric cancer cells

Figure 4 ARHGAP11A interacts with TPM1 in gastric cancer cells. (a) Western blot analysis verified the overexpression efficiency of ARHGAP11A in gastric cancer cells. (b) Western blot detection after IP. (c) Matching peptides in ARHGAP11A and TPM1. (d) Left: Flag-tagged ARHGAP11A and HA-tagged TPM1 plasmids were co-transfected into HEK293T cells for 36 h followed by cell lysate preparation and IP assay with anti-Flag beads followed by immunoblotting with indicated antibodies. Right: HA-tagged ARHGAP11A and Flag-tagged TPM1 plasmids were cotransfected into HEK293T cells for 36 h followed by cell lysate preparation and IP assay with anti-Flag beads followed by immunoblotting with indicated antibodies. IP: immunoprecipitates, WCL: whole-cell lysates. (e) The interaction of ARHGAP11A and TPM1 was tested in AGS cells. AGS cells overexpressing Flag-ARHGAP11A were lysed with cell lysate, followed by IP assay with anti-Flag beads followed by immunoblotting with indicated antibodies. (f) ARHGAP11A domain. (g) Analysis of the domain involved in the interaction between ARHGAP11A and TPM1. HEK293T cells were transiently cotransfected with plasmids expressing Flag-tagged of indicated ARHGAP11A mutant plasmids and HA-tagged TPM1 plasmids, followed by cell lysate preparation and IP assay with anti-Flag beads followed by immunoblotting with indicated antibodies. IP: immunoprecipitates, WCL: whole-cell lysates.

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