Product: ARHGDIG Antibody
Catalog: DF4424
Description: Rabbit polyclonal antibody to ARHGDIG
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine
Mol.Wt.: 25 KD; 25kD(Calculated).
Uniprot: Q99819
RRID: AB_2836779

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(89%), Bovine(100%)
Clonality:
Polyclonal
Specificity:
ARHGDIG Antibody detects endogenous levels of total ARHGDIG.
RRID:
AB_2836779
Cite Format: Affinity Biosciences Cat# DF4424, RRID:AB_2836779.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Arhgdig; GDIR3_HUMAN; OTTHUMP00000067353; OTTHUMP00000194914; Rho GDI 3; Rho GDI gamma; Rho GDP dissociation inhibitor (GDI) gamma; Rho GDP dissociation inhibitor 3; Rho GDP dissociation inhibitor gamma; Rho GDP-dissociation inhibitor 3; Rho-GDI gamma; RHOGDI 3; RhoGDI gamma; RHOGDI3;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q99819 GDIR3_HUMAN:

Primarily expressed in pancreas and brain.

Sequence:
MLGLDACELGAQLLELLRLALCARVLLADKEGGPPAVDEVLDEAVPEYRAPGRKSLLEIRQLDPDDRSLAKYKRVLLGPLPPAVDPSLPNVQVTRLTLLSEQAPGPVVMDLTGDLAVLKDQVFVLKEGVDYRVKISFKVHREIVSGLKCLHHTYRRGLRVDKTVYMVGSYGPSAQEYEFVTPVEEAPRGALVRGPYLVVSLFTDDDRTHHLSWEWGLCICQDWKD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Pig
89
Horse
78
Dog
78
Xenopus
67
Chicken
67
Sheep
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Inhibits GDP/GTP exchange reaction of RhoB. Interacts specifically with the GDP- and GTP-bound forms of post-translationally processed Rhob and Rhog proteins, both of which show a growth-regulated expression in mammalian cells. Stimulates the release of the GDP-bound but not the GTP-bound RhoB protein. Also inhibits the GDP/GTP exchange of RhoB but shows less ability to inhibit the dissociation of prebound GTP.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Primarily expressed in pancreas and brain.

Family&Domains:

Belongs to the Rho GDI family.

Research Fields

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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