ALDH1A2 Antibody - #DF4422

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Product: ALDH1A2 Antibody
Catalog: DF4422
Description: Rabbit polyclonal antibody to ALDH1A2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 52 KD; 57kD(Calculated).
Uniprot: O94788
RRID: AB_2836777

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
ALDH1A2 Antibody detects endogenous levels of total ALDH1A2.
RRID:
AB_2836777
Cite Format: Affinity Biosciences Cat# DF4422, RRID:AB_2836777.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AL1A2_HUMAN; Aldehyde dehydrogenase family 1 member A2; ALDH1A2 aldehyde dehydrogenase 1 family, member A2; ALDH1A2; Aldh1a7; AV116159; MGC26444; RALDH 2; RALDH(II); Raldh1; RalDH2; RALDH2 T; Retinal dehydrogenase 2; Retinaldehyde dehydrogenase 2; Retinaldehyde specific dehydrogenase type 2; Retinaldehyde-specific dehydrogenase type 2;

Immunogens

Immunogen:
Uniprot:

O94788(AL1A2_HUMAN) >>Visit HPA database.

Gene(ID):
ALDH1A2(8854)
Sequence:
MTSSKIEMPGEVKADPAALMASLHLLPSPTPNLEIKYTKIFINNEWQNSESGRVFPVYNPATGEQVCEVQEADKADIDKAVQAARLAFSLGSVWRRMDASERGRLLDKLADLVERDRAVLATMESLNGGKPFLQAFYVDLQGVIKTFRYYAGWADKIHGMTIPVDGDYFTFTRHEPIGVCGQIIPWNFPLLMFAWKIAPALCCGNTVVIKPAEQTPLSALYMGALIKEAGFPPGVINILPGYGPTAGAAIASHIGIDKIAFTGSTEVGKLIQEAAGRSNLKRVTLELGGKSPNIIFADADLDYAVEQAHQGVFFNQGQCCTAGSRIFVEESIYEEFVRRSVERAKRRVVGSPFDPTTEQGPQIDKKQYNKILELIQSGVAEGAKLECGGKGLGRKGFFIEPTVFSNVTDDMRIAKEEIFGPVQEILRFKTMDEVIERANNSDFGLVAAVFTNDINKALTVSSAMQAGTVWINCYNALNAQSPFGGFKMSGNGREMGEFGLREYSEVKTVTVKIPQKNS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
90
Zebrafish
80
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O94788 As Substrate

Site PTM Type Enzyme
S22 Phosphorylation
K108 Ubiquitination
Y168 Phosphorylation
T170 Phosphorylation
T172 Phosphorylation
K269 Ubiquitination
S278 Phosphorylation
T284 Phosphorylation
Y333 Phosphorylation
S351 Phosphorylation
K365 Ubiquitination
K366 Ubiquitination
K370 Ubiquitination
K384 Acetylation
K384 Ubiquitination
K390 Acetylation
K390 Ubiquitination
K395 Ubiquitination
K415 Ubiquitination
K429 Ubiquitination
K507 Ubiquitination
K512 Ubiquitination

Research Backgrounds

Function:

Converts retinaldehyde to retinoic acid. Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Can metabolize octanal and decanal, but has only very low activity with benzaldehyde, acetaldehyde and propanal. Displays complete lack of activity with citral (By similarity).

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homotetramer.

Family&Domains:

Belongs to the aldehyde dehydrogenase family.

Research Fields

· Metabolism > Metabolism of cofactors and vitamins > Retinol metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Transferrin-dependent crosstalk between the intestinal tract and commensal microbes contributes for immune tolerance. bioRxiv, 2020

Application: WB    Species: Mouse    Sample: gut tissue

Figure 1. Transferrin is a potential contributory factor for intestinal tolerance dependent on gut microbiota. (A) Transferrin concentration in plasma of germ-free (GF) and specific pathogen-free (SPF) (control) mice determined by ELISA. Data represent means ± SD (n = 10), *p < 0.05, **p < 0.01 by one-way ANOVA with Dunnett’s post-hoc test. Western blotting (left) and quantification (right) analyses of transferrin levels in protein extracts from liver (B) and spleen (C) of SPF and GF mice. β-actin was used as the control. Data represent means ± SD (n = 10), *p < 0.05, **p < 0.01 by one-way ANOVA with Dunnett’s post-hoc test (B, C). (D-E) Transferrin, ALDH1A2, CCL22, TGF-β, and IL-10 levels in different gut tissue segments (duodenum, jejunum, ileum, caecum, and colon (D, J, I, C1, and C2)) of SPF (NC), GF (D), and broad-spectrum antibiotic (Abs)-treated SPF (E) mice were determined by Western blotting (left) and quantification (right) analyses. GAPDH was used as the control. Data represent means ± SD (n = 8),

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