RGS10 Antibody - #DF4414

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Product: RGS10 Antibody
Catalog: DF4414
Description: Rabbit polyclonal antibody to RGS10
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 20 KD; 21kD(Calculated).
Uniprot: O43665
RRID: AB_2836769

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
RGS10 Antibody detects endogenous levels of total RGS10.
RRID:
AB_2836769
Cite Format: Affinity Biosciences Cat# DF4414, RRID:AB_2836769.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Regulator of G protein signaling 10; Regulator of G-protein signaling 10; RGS10; RGS10_HUMAN;

Immunogens

Immunogen:
Uniprot:

O43665(RGS10_HUMAN) >>Visit HPA database.

Gene(ID):
RGS10(6001)
Sequence:
MFNRAVSRLSRKRPPSDIHDSDGSSSSSHQSLKSTAKWAASLENLLEDPEGVKRFREFLKKEFSEENVLFWLACEDFKKMQDKTQMQEKAKEIYMTFLSSKASSQVNVEGQSRLNEKILEEPHPLMFQKLQDQIFNLMKYDSYSRFLKSDLFLKHKRTEEEEEDLPDAQTAAKRASRIYNT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
92
Xenopus
67
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43665 As Substrate

Site PTM Type Enzyme
S16 Phosphorylation
S24 Phosphorylation
S176 Phosphorylation

Research Backgrounds

Function:

Regulates G protein-coupled receptor signaling cascades, including signaling downstream of the muscarinic acetylcholine receptor CHRM2. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. Modulates the activity of potassium channels that are activated in response to CHRM2 signaling. Activity on GNAZ is inhibited by palmitoylation of the G-protein.

Subcellular Location:

Cytoplasm>Cytosol. Nucleus.
Note: Forskolin treatment promotes phosphorylation and translocation to the nucleus.

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with GNAZ, GNAI1 and GNAI3. Associates specifically with the activated, GTP-bound forms of GNAZ and GNAI3.

References

1). miR‑199b‑5p mediates adriamycin‑induced podocyte apoptosis by inhibiting the expression of RGS10. Experimental and Therapeutic Medicine, 2021 (PubMed: 34737809) [IF=2.4]

Application: WB    Species: Mouse    Sample: podocytes

Figure 3 miR-199b-5p regulates the expression of RGS10. (A) Predicted binding sites of miR-199b-5p on the 3'UTR of RGS10. (B) RGS10 expression level after transient transfection and treatment of podocytes with miR-199b-5p mimic, NC mimic, ADR, ADR+miR-199b-5p mimic or ADR+NC mimic. (C) RGS10 expression level after the transient transfection and treatment of podocytes with miR-199b-5p inhibitor, NC inhibitor, ADR, ADR+miR-199b-5p inhibitor or ADR+NC inhibitor. (D) RGS10 protein expression in podocytes treated as in (B). (E) RGS10 protein expression in podocytes treated as in (C). (F) Expression level of RGS10 was determined by RT-qPCR in ADR-treated podocytes transfected with miR-199b-5p mimic or miR-199b-5p mimic+AdRGS10. (G) Expression of Bax and Bcl-2 was determined by western blotting in ADR-treated podocytes transfected as in (F). Control indicates normal podocytes. n=3 in each group. *P

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