RAP2C Antibody - #DF4411

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Product: RAP2C Antibody
Catalog: DF4411
Description: Rabbit polyclonal antibody to RAP2C
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 26 KD; 21kD(Calculated).
Uniprot: Q9Y3L5
RRID: AB_2836766

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
RAP2C Antibody detects endogenous levels of total RAP2C.
RRID:
AB_2836766
Cite Format: Affinity Biosciences Cat# DF4411, RRID:AB_2836766.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2010200P20Rik; AI194294; AL022976; BOS_25229; DKFZp313B211; MGC143331; OTTHUMP00000024037; OTTMUSP00000019067; RAP2C; RAP2C, member of RAS oncogene family; RAP2C_HUMAN; Ras related protein Rap 2c; Ras-related protein Rap-2c; RP23-15D12.2;

Immunogens

Immunogen:
Uniprot:

Q9Y3L5(RAP2C_HUMAN) >>Visit HPA database.

Gene(ID):
RAP2C(57826)
Expression:
Q9Y3L5 RAP2C_HUMAN:

Expressed in liver, skeletal muscle, prostate, uterus, rectum, stomach, and bladder and to a lower extent in brain, kidney, pancreas, and bone marrow. Expressed in mononuclear leukocytes and megakaryocytes.

Sequence:
MREYKVVVLGSGGVGKSALTVQFVTGTFIEKYDPTIEDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDLYIKNGQGFILVYSLVNQQSFQDIKPMRDQIVRVKRYEKVPLILVGNKVDLEPEREVMSSEGRALAQEWGCPFMETSAKSKSMVDELFAEIVRQMNYSSLPEKQDQCCTTCVVQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Zebrafish
90
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y3L5 As Substrate

Site PTM Type Enzyme
Y4 Phosphorylation
K5 Ubiquitination
S11 Phosphorylation
S17 Phosphorylation
T20 Phosphorylation
T25 Phosphorylation
T27 Phosphorylation
K31 Ubiquitination
Y40 Phosphorylation
K42 Ubiquitination
T61 Phosphorylation
S66 Phosphorylation
Y106 Phosphorylation
K108 Ubiquitination
K117 Ubiquitination
K148 Ubiquitination
Y166 Phosphorylation

Research Backgrounds

Function:

Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May play a role in SRE-mediated gene transcription.

PTMs:

Palmitoylated. Palmitoylation is required for association with recycling endosome membranes and activation of TNIK.

Subcellular Location:

Cytoplasm. Recycling endosome membrane>Lipid-anchor>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in liver, skeletal muscle, prostate, uterus, rectum, stomach, and bladder and to a lower extent in brain, kidney, pancreas, and bone marrow. Expressed in mononuclear leukocytes and megakaryocytes.

Family&Domains:

Belongs to the small GTPase superfamily. Ras family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

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