RAB5C Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from various samples, using RAB5C Antibody.

Product:	RAB5C Antibody
Catalog:	DF4406
Description:	Rabbit polyclonal antibody to RAB5C
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.:	30 KD; 23kD(Calculated).
Uniprot:	P51148
RRID:	AB_2836761

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(90%)

Clonality:

Polyclonal

Specificity:

RAB5C Antibody detects endogenous levels of total RAB5C.

RRID:

AB_2836761
Cite Format: Affinity Biosciences Cat# DF4406, RRID:AB_2836761.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

L1880; RAB, member of RAS oncogene family like; RAB5C; RAB5C, member of RAS oncogene family; RAB5C, member RAS oncogene family; RAB5C_HUMAN; RAB5CL; RAB5L; RABL; Ras-related protein Rab-5C;

Immunogens

Immunogen:

Uniprot:

P51148(RAB5C_HUMAN) >>Visit HPA database.

Gene(ID):

RAB5C(5878)

Sequence:

P51148 RAB5C_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGRGGAARPNGPAAGNKICQFKLVLLGESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNTDTFARAKNWVKELQRQASPNIVIALAGNKADLASKRAVEFQEAQAYADDNSLLFMETSAKTAMNVNEIFMAIAKKLPKNEPQNATGAPGRNRGVDLQENNPASRSQCCSN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51148 As Substrate

P51148

Site	PTM Type	Source
R4	Methylation	Uniprot
R9	Methylation	Uniprot
K18	Ubiquitination	Uniprot
K23	Acetylation	Uniprot
S30	Phosphorylation	Uniprot
K34	Ubiquitination	Uniprot
Y83	Phosphorylation	Uniprot
S85	Phosphorylation	Uniprot
K117	Ubiquitination	Uniprot
S124	Phosphorylation	Uniprot
K135	Ubiquitination	Uniprot
S140	Phosphorylation	Uniprot
K141	Ubiquitination	Uniprot
K180	Ubiquitination	Uniprot
S209	Phosphorylation	Uniprot

Research Backgrounds

P51148_RAB5C_HUMAN

Function:

Protein transport. Probably involved in vesicular traffic (By similarity).

PTMs:

Phosphorylation of Ser-85 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.

Subcellular Location:

Cell membrane>Lipid-anchor>Cytoplasmic side. Early endosome membrane>Lipid-anchor. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Subunit Structure:

Interacts with EEA1. Interacts with INCA1. Interacts with GDI1, GDI2, CHML and CHM; phosphorylation at Ser-85 disrupts this interaction.

Family&Domains:

Belongs to the small GTPase superfamily. Rab family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.

Restrictive clause

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For Research Use Only.
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RAB5C Antibody - #DF4406