RASA3 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Rat brain, using RASA3 Antibody at 1/1000 dilution.

Product:	RASA3 Antibody
Catalog:	DF4386
Description:	Rabbit polyclonal antibody to RASA3
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Dog, Chicken
Mol.Wt.:	96 KD; 96kD(Calculated).
Uniprot:	Q14644
RRID:	AB_2836741

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(88%), Bovine(88%), Dog(88%), Chicken(88%)

Clonality:

Polyclonal

Specificity:

RASA3 Antibody detects endogenous levels of total RASA3.

RRID:

AB_2836741
Cite Format: Affinity Biosciences Cat# DF4386, RRID:AB_2836741.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

GAP III; GAP1(IP4BP); GAP1IP4BP; GAPIII; GTPase activating protein 1 family; GTPase activating protein III; Inositol 1 3 4 5 tetrakisphosphate binding protein; Inositol 1345 tetrakisphosphate binding protein; Ins P4 binding protein; Ins P4-binding protein; Ins(1 3 4 5)P4 binding protein; Ins(1345)P4 binding protein; MGC46517; MGC47588; Ras GTPase activating protein 3; Ras GTPase-activating protein 3; RAS p21 protein activator 3; RASA 3; RASA3; RASA3_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q14644(RASA3_HUMAN) >>Visit HPA database.

Gene(ID):

RASA3(22821)

Sequence:

Q14644 RASA3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAVEDEGLRVFQSVKIKIGEAKNLPSYPGPSKMRDCYCTVNLDQEEVFRTKIVEKSLCPFYGEDFYCEIPRSFRHLSFYIFDRDVFRRDSIIGKVAIQKEDLQKYHNRDTWFQLQHVDADSEVQGKVHLELRLSEVITDTGVVCHKLATRIVECQGLPIVNGQCDPYATVTLAGPFRSEAKKTKVKRKTNNPQFDEVFYFEVTRPCSYSKKSHFDFEEEDVDKLEIRVDLWNASNLKFGDEFLGELRIPLKVLRQSSSYEAWYFLQPRDNGSKSLKPDDLGSLRLNVVYTEDHVFSSDYYSPLRDLLLKSADVEPVSASAAHILGEVCREKQEAAVPLVRLFLHYGRVVPFISAIASAEVKRTQDPNTIFRGNSLASKCIDETMKLAGMHYLHVTLKPAIEEICQSHKPCEIDPVKLKDGENLENNMENLRQYVDRVFHAITESGVSCPTVMCDIFFSLREAAAKRFQDDPDVRYTAVSSFIFLRFFAPAILSPNLFQLTPHHTDPQTSRTLTLISKTVQTLGSLSKSKSASFKESYMATFYEFFNEQKYADAVKNFLDLISSSGRRDPKSVEQPIVLKEGFMIKRAQGRKRFGMKNFKKRWFRLTNHEFTYHKSKGDQPLYSIPIENILAVEKLEEESFKMKNMFQVIQPERALYIQANNCVEAKDWIDILTKVSQCNQKRLTVYHPSAYLSGHWLCCRAPSDSAPGCSPCTGGLPANIQLDIDGDRETERIYSLFNLYMSKLEKMQEACGSKSVYDGPEQEEYSTFVIDDPQETYKTLKQVIAGVGALEQEHAQYKRDKFKKTKYGSQEHPIGDKSFQNYIRQQSETSTHSI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Bovine

Dog

Chicken

Horse

Sheep

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14644 As Substrate

Q14644

Site	PTM Type	Source
A2	Acetylation	Uniprot
S13	Phosphorylation	Uniprot
K15	Ubiquitination	Uniprot
K32	Ubiquitination	Uniprot
K55	Ubiquitination	Uniprot
Y61	Phosphorylation	Uniprot
Y66	Phosphorylation	Uniprot
S77	Phosphorylation	Uniprot
Y79	Phosphorylation	Uniprot
S90	Phosphorylation	Uniprot
K99	Ubiquitination	Uniprot
K104	Ubiquitination	Uniprot
T110	Phosphorylation	Uniprot
K146	Ubiquitination	Uniprot
Y199	Phosphorylation	Uniprot
T203	Phosphorylation	Uniprot
S207	Phosphorylation	Uniprot
Y208	Phosphorylation	Uniprot
K223	Acetylation	Uniprot
K237	Ubiquitination	Uniprot
K251	Ubiquitination	Uniprot
S257	Phosphorylation	Uniprot
K273	Ubiquitination	Uniprot
K276	Ubiquitination	Uniprot
S282	Phosphorylation	Uniprot
K309	Ubiquitination	Uniprot
Y345	Phosphorylation	Uniprot
K378	Ubiquitination	Uniprot
T450	Phosphorylation	Uniprot
T511	Phosphorylation	Uniprot
T518	Phosphorylation	Uniprot
T521	Phosphorylation	Uniprot
S524	Phosphorylation	Uniprot
S526	Phosphorylation	Uniprot
K527	Acetylation	Uniprot
K527	Ubiquitination	Uniprot
K529	Acetylation	Uniprot
S532	Phosphorylation	Uniprot
K555	Ubiquitination	Uniprot
S562	Phosphorylation	Uniprot
S563	Phosphorylation	Uniprot
S564	Phosphorylation	Uniprot
K570	Ubiquitination	Uniprot
K579	Ubiquitination	Uniprot
K596	Acetylation	Uniprot
K599	Acetylation	Uniprot
K616	Ubiquitination	Uniprot
K634	Ubiquitination	Uniprot
K641	Ubiquitination	Uniprot
K643	Ubiquitination	Uniprot
K674	Ubiquitination	Uniprot
S703	Phosphorylation	Uniprot
K746	Ubiquitination	Uniprot
Y757	Phosphorylation	Uniprot
T776	Phosphorylation	Uniprot
Y777	Phosphorylation	Uniprot
K778	Ubiquitination	Uniprot
K781	Ubiquitination	Uniprot
Y797	Phosphorylation	Uniprot
T805	Phosphorylation	Uniprot
K806	Ubiquitination	Uniprot
Y807	Phosphorylation	Uniprot
S809	Phosphorylation	Uniprot
K817	Ubiquitination	Uniprot
S818	Phosphorylation	Uniprot
Y822	Phosphorylation	Uniprot
S827	Phosphorylation	Uniprot
T829	Phosphorylation	Uniprot
S830	Phosphorylation	Uniprot
T831	Phosphorylation	Uniprot
S833	Phosphorylation	Uniprot

Research Backgrounds

Q14644_RASA3_HUMAN

Function:

Inhibitory regulator of the Ras-cyclic AMP pathway. Binds inositol tetrakisphosphate (IP4) with high affinity. Might be a specific IP4 receptor.

Subcellular Location:

Cell membrane.

Research Fields

· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)

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RASA3 Antibody - #DF4386