RIN1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from K562 cells, using RIN1 antibody.

Western blot analysis of extracts from Hela cells, using RIN1 Antibody.

Product:	RIN1 Antibody
Catalog:	DF4384
Description:	Rabbit polyclonal antibody to RIN1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Dog
Mol.Wt.:	84 KD; 84kD(Calculated).
Uniprot:	Q13671
RRID:	AB_2836739

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(80%), Horse(90%), Dog(90%)

Clonality:

Polyclonal

Specificity:

RIN1 Antibody detects endogenous levels of total RIN1.

RRID:

AB_2836739
Cite Format: Affinity Biosciences Cat# DF4384, RRID:AB_2836739.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Ras and Rab interactor 1; ras inhibitor 1; ras inhibitor; Ras inhibitor JC99; ras inhibitor RIN1; Ras interaction/interference protein 1; RIN 1;

Immunogens

Immunogen:

Uniprot:

Q13671(RIN1_HUMAN) >>Visit HPA database.

Gene(ID):

RIN1(9610)

Expression:

Q13671 RIN1_HUMAN:

Expressed in all tissues examined with high levels in brain, placenta and pancreas.

Sequence:

Q13671 RIN1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MESPGESGAGSPGAPSPSSFTTGHLAREKPAQDPLYDVPNASGGQAGGPQRPGRVVSLRERLLLTRPVWLQLQANAAAALHMLRTEPPGTFLVRKSNTRQCQALCMRLPEASGPSFVSSHYILESPGGVSLEGSELMFPDLVQLICAYCHTRDILLLPLQLPRAIHHAATHKELEAISHLGIEFWSSSLNIKAQRGPAGGPVLPQLKARSPQELDQGTGAALCFFNPLFPGDLGPTKREKFKRSFKVRVSTETSSPLSPPAVPPPPVPVLPGAVPSQTERLPPCQLLRRESSVGYRVPAGSGPSLPPMPSLQEVDCGSPSSSEEEGVPGSRGSPATSPHLGRRRPLLRSMSAAFCSLLAPERQVGRAAAALMQDRHTAAGQLVQDLLTQVRAGPEPQELQGIRQALSRARAMLSAELGPEKLLSPKRLEHVLEKSLHCSVLKPLRPILAARLRRRLAADGSLGRLAEGLRLARAQGPGAFGSHLSLPSPVELEQVRQKLLQLLRTYSPSAQVKRLLQACKLLYMALRTQEGEGAGADEFLPLLSLVLAHCDLPELLLEAEYMSELLEPSLLTGEGGYYLTSLSASLALLSGLGQAHTLPLSPVQELRRSLSLWEQRRLPATHCFQHLLRVAYQDPSSGCTSKTLAVPPEASIATLNQLCATKFRVTQPNTFGLFLYKEQGYHRLPPGALAHRLPTTGYLVYRRAEWPETQGAVTEEEGSGQSEARSRGEEQGCQGDGDAGVKASPRDIREQSETTAEGGQGQAQEGPAQPGEPEAEGSRAAEE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Dog

Pig

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13671 As Substrate

Q13671

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
S3	Phosphorylation		Uniprot
S11	Phosphorylation		Uniprot
S16	Phosphorylation		Uniprot
K29	Ubiquitination		Uniprot
Y36	Phosphorylation	P42684 (ABL2)	Uniprot
S42	Phosphorylation		Uniprot
T65	Phosphorylation		Uniprot
T90	Phosphorylation		Uniprot
K207	Ubiquitination		Uniprot
S210	Phosphorylation		Uniprot
T218	Phosphorylation		Uniprot
T236	Phosphorylation		Uniprot
K237	Ubiquitination		Uniprot
S244	Phosphorylation		Uniprot
T251	Phosphorylation		Uniprot
T253	Phosphorylation		Uniprot
S255	Phosphorylation		Uniprot
S258	Phosphorylation		Uniprot
S291	Phosphorylation		Uniprot
S292	Phosphorylation	Q9BZL6 (PRKD2) , Q15139 (PRKD1)	Uniprot
Y295	Phosphorylation		Uniprot
S330	Phosphorylation		Uniprot
S333	Phosphorylation		Uniprot
T336	Phosphorylation		Uniprot
S337	Phosphorylation		Uniprot
S349	Phosphorylation		Uniprot
S351	Phosphorylation	P98161 (PKD1) , Q15139 (PRKD1)	Uniprot
T377	Phosphorylation		Uniprot
T388	Phosphorylation		Uniprot
S407	Phosphorylation		Uniprot
S424	Phosphorylation		Uniprot
K513	Ubiquitination		Uniprot
S609	Phosphorylation		Uniprot
S611	Phosphorylation		Uniprot
Y632	Phosphorylation		Uniprot
R664	Methylation		Uniprot
Y676	Phosphorylation		Uniprot
Y681	Phosphorylation		Uniprot
R692	Methylation		Uniprot
T696	Phosphorylation		Uniprot
Y698	Phosphorylation		Uniprot
Y701	Phosphorylation		Uniprot
K742	Ubiquitination		Uniprot
S744	Phosphorylation		Uniprot

Research Backgrounds

Q13671_RIN1_HUMAN

Function:

Ras effector protein, which may serve as an inhibitory modulator of neuronal plasticity in aversive memory formation. Can affect Ras signaling at different levels. First, by competing with RAF1 protein for binding to activated Ras. Second, by enhancing signaling from ABL1 and ABL2, which regulate cytoskeletal remodeling. Third, by activating RAB5A, possibly by functioning as a guanine nucleotide exchange factor (GEF) for RAB5A, by exchanging bound GDP for free GTP, and facilitating Ras-activated receptor endocytosis.

PTMs:

Phosphorylated on tyrosine residues by ABL1 and ABL2. Phosphorylation at Ser-351 by PRKD1 induces interaction with 14-3-3 proteins.

Subcellular Location:

Cytoplasm. Membrane. Cytoplasm>Cytoskeleton.
Note: Some amount is membrane-associated.

Tissue Specificity:

Expressed in all tissues examined with high levels in brain, placenta and pancreas.

Subunit Structure:

Interacts with the GTP-bound form of Ras proteins (NRAS, HRAS and KRAS). This interaction prevents the association between RAF1 and Ras. Interacts with 14-3-3 proteins YWHAB, YWHAE and YWHAZ when phosphorylated on Ser-351. Interacts with the SH3 domain of ABL1 and ABL2. Interacts with RAB5A. The interaction with Ras is probably regulated and antagonized by the interaction with 14-3-3 proteins. The interaction with 14-3-3 proteins is regulated by phosphorylation on Ser-351.

Family&Domains:

Belongs to the RIN (Ras interaction/interference) family.

Research Fields

· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)

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RIN1 Antibody - #DF4384